Reshaping life starting from the Junior Class of HKUST

Chapter 155 Liar Wu Zhe
In the next few days, Wu Zhe buried himself in the laboratory.Kaixin Network was going to hold an auction two days later, but some domestic investment institutions have requested to arrange the auction on the day before New Year's Day, so that they can have more time to raise funds.After Guo Ziyi asked Wu Zhe for instructions, he also agreed to this request.

Wu Zhe didn't feel much relieved, he finally figured out the program, and then threw the program to Zheng Yizhong.Let him keep inputting the previous experimental data to check the correct rate.

And he began to perform Sanger sequencing in the laboratory wearing a sterile suit.After he was taken by Tang Meng to do it twice, he was able to operate independently.

First, DNA polymerase is used to extend the primer bound to the template of the undetermined sequence.until a chain-terminating nucleotide is incorporated.

Each sequence determination consists of a set of four separate reactions, each reaction containing all four deoxynucleotide triphosphates (dNTPs), mixed with a limited amount of a different dideoxynucleoside triphosphate (ddNTP).Since ddNTPs lack the 3-OH group required for elongation, elongated oligonucleotides are selectively terminated at G, A, T, or C.

The termination point is determined by the corresponding dideoxygen in the reaction.The relative concentration of each dNTPs and ddNTPs can be adjusted to allow the reaction to produce a set of chain-terminated products with a length of several hundred to several thousand bases.

They have a common starting point but end at different nucleotides, and fragments of different sizes can be separated by high-resolution denaturing gel electrophoresis, and can be detected by X-ray film autoradiography after gel processing.

In this way, there are many steps for sequencing, including: adding buffer solvent, centrifugation, thermal cycling, gel plate preparation to final electrophoresis.Even with Wu Zhe's control, he cannot be 100% sure that he will succeed every time.After these successes, data analysis and comparison are needed.

Moreover, many DNA extracts, including small-scale preparations of plasmids, cannot give a reliable estimate of DNA concentration. Mixed chromosomal DNA, protein, RNA, organic and inorganic compounds may have light absorption at 260 nm, and ribonuclease treatment The ribonucleotides produced, although they are in front of the DNA sample after electrophoresis, cannot be observed.This further slowed down the progress of the experiment.

Wu Zhejin has done repeated research, and the mathematical model can only treat the symptoms but not the root cause.If you don't directly change the experiment itself, it won't have much effect on the essence.

Wu Zhe left the laboratory, and he has no good solution now.If it is improved, where should we start? There is no way to do the previous method for the time being. Is there anything that can be changed in the gel plate and electrophoresis?

Wu Zhe knew that there was a new sequencing method later, because for a while, commercial activities related to genetic testing were flying all over the sky.Later, the country also introduced regulations.Crack down on criminals who make huge profits in this way.He vaguely remembered that it should be related to the improvement of the chip.But how to connect it with the chip, he is also at a loss now.

Frowning, he walked into the office.

Tang dreamed that Wu Zhe came in, and stepped forward to smooth Wu Zhe's eyebrows with a smile.

Tang Meng asked: "The experiment was not successful? Why are you still frowning? You, you just entered too few laboratories. You will get used to it after doing it a few times. Ask Senior Brother Zheng if he came here like this."

When Zheng Yizhong heard Tang Meng calling him, he also said vaguely: "Well, that's right."

In the past two days, he has been crushed to death by these two couples, testing the data all night every day, and he will still be in a trance.But who made him lose weight significantly in the past two days?Wu Zhe's traditional Chinese medicine is still very effective.

It's just that Wu Zhe and Tang Meng sprinkled dog food from time to time, and he couldn't stand it.In my heart, I became more and more determined to lose weight, and then I also wanted to find someone with long legs and a fierce mask.Cough cough, I can't think about it, think about it, Halazi is about to come down.

"No, it succeeded, but the efficiency is too low. If I have to think of a way, I don't have a good solution right now." Wu Zhe replied.

After hearing Wu Zhe's answer, Zheng Yizhong raised his sleepy eyes. He was already fat and his eyes looked small, which would become a line. After watching Wu Zhe for a while, he said:
"Do you want to do this? Ah Zhe, you have only been here for a few days! The set of procedures that you have come up with now is already perfect. Academician Zhang doesn't know about it yet. If he knows, he will be very happy. I have already tested it. It has been two days, and the calculated data completely match the experimental data, which can be said to be a success. This has to be reported, and a large sum of money cannot be escaped by the bonus. You also want to come up with a new sequencing method. If it succeeds, you can do it I have applied for the Nobel Prize in Physiology. The Lancet journals have to issue a special issue for you."

After Wu Zhela and Tang Meng sat down together, she joked: "At that time, I will add your name, brother, and let you go to Sweden to participate in the Nobel Prize together."

"There is everything in the dream, but I haven't slept for two days, and I haven't dreamed." Zheng Yizhong muttered.

Then he continued: "But what? Thinking about it, it's very beautiful!"

After finishing speaking, he smacked his lips twice and said, "I don't want to get the Nobel Prize, you can get the Lancet! Then I have to add my name to make me famous."

Seeing Zheng Yizhong talking, Wu Zhe knocked his head down.Immediately knew that the fat man was really sleepy.

"Brother, don't worry, if there is such a day, you must be credited! You go back to sleep first. This procedure should be fine." Wu Zhe also said hastily.

Zheng Yizhong also felt that he was reaching the limit, and he was not hypocritical.After packing up my things, I went back to catch up on sleep.

Wu Zhe looked at Zheng Yizhong's far away figure and said to Tang Meng, "Your brother from the same school, except for sometimes speaking incoherently, you are really an honest person."

Tang Meng nodded and said: "Well, brother is very kind. The teacher also likes and respects him very much. The teacher once told me about brother Zheng Yizhong: He said that he was born to eat scientific research, and he may not be as tall as others Wisdom, but his tenacity and ruthlessness are not found in others.”

Wu Zhe also nodded when he heard that, Zheng Yizhong is really ruthless.It can be seen from his weight loss that he not only fulfills what Wu Zhe said every day, but the key point is that this kid consciously eats only one meal a day.The fact that he couldn't stand it for two days is definitely related to the lack of energy in his body now.

"Azhe, there are no side effects from your weight loss method!" Tang Mengmeng couldn't help but ask Wu Zhe when he realized that Zheng Yizhong was desperate.

Wu Zhe shrugged his shoulders and said, "I haven't lost weight, and I don't know. It should probably be fine, at most I'm just hungry."

"What about the diet pill? Is it really that effective?"

Seeing Tang Meng's eager expression, Wu Zhe immediately said: "You said that Chinese medicine! Don't drink it! I want to make a fortune sooner if I want to lose weight. This is a medicine for clearing the body."

Seeing that Tang Meng didn't understand yet, Wu Zhe said bluntly: "That's a laxative, but it doesn't empty you out all at once, and it just makes you go to the bathroom a few more times a day. Now, if Senior Brother Zheng takes one meal a day, it's actually useless." It's useless."

Tang Meng gave Wu Zhe an angry look, and twisted Wu Zhe's waist.

Tang Meng originally wanted to share the unique weight-loss medicine with the little sisters in the dormitory, but Tang Meng remembered it clearly after Wu Zhe reported the name and dosage of the medicine that day.

It's a matter of figure, Tang Meng is not exempt!
What is Wu Zhe, a big liar, doing?

(End of this chapter)