Lu Ming

Chapter 315 Embryo Transfer and Ma Zheng

In theory, it is best for the donor cow and the recipient cow to go into heat on the same day, which can increase the chances of embryo survival.So when the emperor brought the liquid nitrogen tank early this morning, Wang Goode, who had already prepared, selected all the cows in estrus among the buffaloes.

The next step is to defrost.

Wang Goode took Huo Xiong to the laboratory. The two first changed into isolation suits, then they took out the thin tube containing the embryos from the liquid nitrogen, and then immersed the thin tube in warm water at 35°C.

After 20 minutes, the thawed embryos will be diluted several times with 0.3 M sucrose solution. This is done to remove the glycerol in the cells.

The final step is to verify embryo viability.After the two had examined the embryos with a microscope, the preparatory work was completed.

Thanks to the advanced chromosome identification technology of later generations, these selected embryos have a probability of more than 95% to produce cows in the future.Therefore, science and technology are the real reasons behind all the miracles. Wang Goode and the others actually enjoyed the results standing on the shoulders of countless giants.

After preparing the embryos here, the farm workers over there have also pushed the female buffalo into the special barrier.

The parry is a very narrow wooden aisle, and the cow cannot turn around after entering, but can only honestly show the cow's buttocks for surgery.

The previous embryo surgery required an incision in the abdomen of the cow for transplantation.With the continuous innovation of technology over the years, the whole process is now done without a knife. It has been changed to a gun.

After everything was ready, Comrade Wang Goode first came behind the cow, then he let out a soft drink, fell down from the horse, aimed at the cow's buttocks, and stabbed a part of his body in.

After stabbing, Wang Goode looked up at the sky with a tasteful expression on his face.As the pierced part squirms gently, accompanied by the cow's cry of "Mou Mou", the scene is very exciting.The farm workers surrounded by them had never seen such a beautiful scene before, each of them swallowed dryly, and they were all dumbfounded.

It was too late to say it, but it was so fast, after Wang Goode squirmed a few times, accompanied by a low shout of "the gun is coming", Huo Xiong, who was helping at the side, hurriedly handed over the toilet paper. It was a transplant gun.

It was his arm that Wang Goode inserted into the cow's butt just now.

He does this because he first checks which ovary the cow's corpus luteum is on: Cows with hypoplastic corpus luteum cannot be transplanted.

After that, he has to hold the cow's cervix with his hand, and then he has to take over the implantation gun with his other hand, and then poke the tip of the gun into the cow's uterus to inject the embryo.

The entire surgical process must be fast and accurate to avoid damage to the tissue—if you are not careful, you will puncture it.

In one afternoon, the two performed embryo transfer operations on more than 40 cows.

Some of these cows are in estrus, while others have adjusted their estrus through injection of hormones.In short, with the existing conditions of the farm, Wang Goode and the others have done their best.The next step is to wait for the judgment of fate - if more than half of the cows are successfully pregnant, then today is considered a success.

Embryo transfer is a long-term work.It takes ten years to develop a cattle population.

After more than 50 years of introduction and improvement in later generations, the number of Simmental cattle is only 2, and the remaining millions are all hybrid cattle, which shows how difficult it is to increase the number of native populations.

The only good news here is that the frozen embryos are very small, so Cao Chuan can bring a liquid nitrogen tank here from time to time.

As long as there is a sufficient number of pregnant cows here at any time, the magical technique of embryo transfer can continue until the population number is increased to more than a thousand. At that time, it will be possible. Consider setting up sub-bases in the north and south.

Now is the 17th century when the land is vast and the population is sparsely populated. Travelers can completely raise cattle in the enclosure of the Ming Dynasty, not afraid of insufficient land.

After Huo Xiong finished working as a helper, he lived in the farm that day.The next day, he was going to the food company to make a fuss, but who knew that the racecourse called again and asked him to go there.So full of reluctance, Huo Xiong went to the racecourse again on his motorcycle.

When it comes to Ma Zheng, there are many contradictions in it, and we have to start from a long time ago.

First of all, the horse farm was not established by Huo Xiong and others in the livestock industry.It is actually the military who are pushing the construction of the racecourse. To be precise, it is the army.

Here again, strategic issues are involved.

From the perspective of the army personnel, in the next three to five years, at most seven or eight years later, the imperial army in the north of Ming Dynasty will inevitably go through a series of battles, with small-scale public security wars.

The obvious point is: there is no water system extending in all directions in the north of the Ming Dynasty, only the official road that can be called "funny" in the eyes of the crossing people.In this case, cavalry units can be very useful, especially in endless skirmishes.

That's why the army has been planning to form a cavalry unit a long time ago-everyone knows that the army horse population cannot be bred in a year or two.

However, the army's attempt was opposed by everyone.Others are fine, they just oppose for the sake of opposing. Every time you import a horse, there will be less equipment on my side.And the most ruthless stabbing knife in the back of the army is actually the navy.

Navy professionals quickly exposed the army's reputation: For cavalry with breech-loading carbines and indigenous battles, the mode of war has long been no longer a bloody assault saber.

Even if the Imperial Army has cavalry in the future, the combat mode is very easy-get on the horse and trot, get close to the target, dismount and shoot, catch up with the defeated enemy leisurely from behind, and hack with a saber.

Yes, this is the procedure for shooting aborigines with a breech-loading gun.The enemy will be hit and collapsed by the rain of bullets at a distance of 400 meters, and there is no need for a horse to charge.

The so-called elite cavalry has been upgraded to "mountain infantry" with high-tech equipment, which has reduced the demand for expensive and good horses.

In this mode, the Army is making a lot of noise, and the military horses raised by spending huge resources are actually useless-whether it is a draft horse, a bad horse, or even a donkey or a mule, as long as it can carry people to trot, it can achieve tactical purposes. The advantages of military horses is greatly reduced here.

Therefore, the establishment of an elite cavalry unit is a huge pit. The real purpose of the army is to pretend to be aggressive, and use the import quotas obtained from other departments to save food and clothing to go to the north to pretend to be aggressive.

After being appraised by the navy in this way, the cross-border people of various departments suddenly realized that there was a wave of criticism of the army's liar gang.

Seeing that the situation is not good, the army group who encountered the knife-piercing sect in the 17th century could only calm down temporarily, and recorded all the bastards in the small ledger, and retaliated in the future.

It was supposed to be over.As a result, it was so hard to die. In the preparatory meeting before Emperor Cao's ascension to the throne, an idiot proposed the concept of "white horse clears the way".

This is a bad thing.When Emperor Cao heard the words, he stated on the spot that this could be possible, and the army commander Han Xiaobo who was present reacted quickly, and immediately added an advanced concept of enthronement to the emperor of "24 white Arabian horses and silver-armored knights to clear the way".

Although Han Xiaobo didn't succeed in the end, Xia Zhongtang took action himself, forcibly resisting the emperor's displeasure and reducing the number of horses to four.

But after going through this, the door that had been closed was forced open a crack—the cavalry plan of the army turned salty fish, and it was able to continue it again~!

After twists and turns, the military horse farm built by the army was built in such a flickering way, and the location is not far from the cattle farm.

Due to the lack of support from the administrative system, the operation of this racecourse has been very difficult since its establishment.Except for four purebred Arabian horses, the rest are some small Dian horses and draft horses, which are very small.

Although animal husbandry does not usually interfere with the operation of the horse farm, it still has the responsibility of "technical guidance", so Huo Xiong still has to go around if he is fine.

The person in charge of the horse farm is Huang Xu, a muscular black and strong man.This gentleman worked as a cowboy on a western farm before crossing, and also served in the National Guard. He is a compound talent of the Army, and he is also the foundation of the Army's establishment of a military horse farm.

Seeing Huo Xiong, Huang Xu, who was brushing the horse, threw the brush to the horse farm staff, and then he strode over.

After patting Huo Xiong on the shoulder vigorously, Huang Xu took out two punch cigars, one for each of them.

"Hey, not bad." Huo Xiong lit his cigar and took a deep breath: "How about those of you who have stayed in the United States, the enjoyment is different."

"I'm not used to domestic cigarettes. I have a lot of phlegm." After taking a sip of the cigar, Huang Xu didn't go around the bush, and went straight to the topic: "I heard that you had an embryo transfer yesterday?"

Huo Xiong nodded: "Well, I have planted more than 40 heads, and I don't know how the effect will be."

"Then it's time to try it on a horse, right?" Huang Xu said, staring at Huo Xiong with piercing eyes.

"Don't tell me about this." Huo Xiong raised Erlang's legs at this time, leaned on the motorcycle seat, and said to Huang Xu with a smile: "All consumables and labor need to go to the meeting. Can't be the master."

Huang Xu thought to himself, if I can handle the cabinet meeting, why should I ask you?However, he would be asking for something from others, so he naturally had to be more polite when speaking, so he could only chuckle: "You are the head of the animal husbandry company, why don't you help shout a few words at the meeting later?"

"Old Huang, to tell you the truth, you don't need to rely on the Headquarters for this matter." Huo Xiong also opened the skylight to speak bluntly: "I even depend on the breed of the cattle. The emperor's face is just for his convenience. Immediately." It is even more impossible to breed. If you want to expand the population, you have to pass the cabinet."

"Cows and horses have the same strategic value!" Huang Xu was indignant: "Sooner or later, those people in power will find out that they made a mistake!"

Huo Xiong laughed when he heard this: "Hehe, Lao Huang, I didn't say that. At present, the strategic value of horses is really not high."

(End of this chapter)