This doctor has a system

Chapter 110 109. Li Xinyao who wants to lie flat

When Li Xinyao came home, Li's father and Li's mother were eating there.Seeing her daughter coming back, Mama Li quickly took the set of bowls and chopsticks, "Why didn't you tell me earlier when you came back? Where's Gao Feng?"

"He went to the scientific research building. I'm bored there, so I just come here for a while." Li Xinyao put the small backpack on the sofa.

She picked up a few chopsticks of green vegetables, and drank a few mouthfuls of porridge, "I'm full."

"Eat these pieces of fish." Li's mother refused to let her get up, "You eat so little, you don't have any nutrition to give birth to a child."

"I'm not pregnant yet." Li Xinyao felt helpless, so she gnawed on some pieces of fish.

After eating, Li Xinyao sat on the sofa a little absent-minded, and then simply lay on the sofa and watched TV.Dad Li didn't think anything of it, he made a cup of Pu'er and drank it slowly.

Careful Mama Li sensed her daughter's emotions, and she tentatively asked, "Yaoyao, what's wrong with you? Why do you look a little unhappy?"

"It's okay." Li Xinyao replied.

"Just talk about it." Li's father looked at his daughter, "Our life experience is much richer than yours. If you have any confusion, tell me, and Dad will explain it to you."

"Just don't disturb your daughter." Li's mother didn't give him any face. "Do you understand feelings? Do you know what love is?"
.
"I don't understand, do you understand? Do you understand the king?" Father Li retorted.

"What do you know about Wang? What are you talking about?" Li Ma didn't bother to answer him, "Yaoyao, did you have a fight with Gao Feng? Let me tell you that it's normal for two people to quarrel."

"When your dad and I first fell in love, I was so angry that I wanted to vomit blood every day, and this is not all over."

"What do you think he has compared to Gao Feng? He's ugly and poor, so I won't grit my teeth and persevere."

"Dong Yaning, you're going too far!" Father Li didn't drink any tea, and was about to argue with Mother Li, "I didn't want to talk to you back then!"

Li Xinyao laughed, "There's no quarrel, I just don't want to go to work all of a sudden."

"Huh?" Li Ma was dumbfounded, "How long have you been working? You don't want to do it now?"

"Did you feel wronged in the department?" Father Li felt that the matter was a bit serious.

"No, I just want to lie flat." Li Xinyao said.

"Is there really no other reason?" Mama Li confirmed.

"No."

"That's good." Li Ma turned and went back to the house to get a feather duster.

"Mom, what are you doing?" Li Xinyao was anxious, and quickly stood up from the sofa.

"Let you wake up!" Mama Li greeted her with a feather duster, "It's against you, you're not married yet, you just want to do it, see if I don't kill you, so you don't marry someone who throws me away. "

Li Xinyao quickly hid behind Papa Li, "Mom, listen to me, the main reason is that there is no point in my going to work, and I don't earn that little money for a year."

"Nonsense! Your salary is almost higher than that of your dad and I." Li Mama greeted me with a stick, and Li Dad took a hard hit, "Look carefully, and hit when you are sure! "He was in a hurry.

"Gao Feng earned a lot of money." Li Xinyao said quickly, "I think I might as well be a housewife at home."

Mama Li stopped abruptly, "You'd better tell me clearly, or I'll kill you soon."

Li Xinyao carefully explained the situation to her parents, "The contract has been signed, and the first payment has been sent."

"I also transferred 100 million yuan to me." At the end, she added a sentence, and showed the bank card balance to the two of them.

"You're not married yet, how can you take so much money from someone else?" Father Li choked out a sentence for a long time.

Li's mother gave him a dissatisfied look, "We all live together, what's the difference with getting married, if you can't speak, don't express your opinion!"

She took her daughter to sit down, "Anyway, you should go to work and you have to go to work. If you have a baby in the future, let's talk about what happens."

"How about your doctorate is not for nothing." Father Li interjected.

"Don't mention it, it's useless to get a doctorate." Li Xinyao was depressed, "I applied for a project in the province, but it was directly rejected. I suspect that people didn't read it carefully."

Almost all those at her age who dare to apply for a project have a doctoral degree, and the competition is fierce.She was very open before, but recently, being stimulated by her boyfriend, she wrote an application topic and submitted it with high spirits. As expected, she suffered a great psychological blow.

So the idea of ​​retiring and lying flat was born.

"Hey, what's your rivalry with Gao Feng? If he's good, you'll be happy. How can the two of you be so good? How can others live like that?" Li Ma persuaded her daughter.

"Go to your class well. It's great to have a job. No one else will envy you." Dad Li also educated.

When Gao Feng came to the scientific research building here, Li Youliang, Hu Jiaran, Cheng Song, and Qi Nannan were all there. Since the money had just been distributed, everyone couldn't hide the smiles on their faces.

Especially when Qi Nannan and Cheng Song knew that this was only the first payment, their voices trembled a little.

Qi Nannan was very gentle when facing the little white mice today, and even wiped the cages carefully, so it doesn't smell bad anymore.

"Two things, one is that we need to register a company, and we may have to deal with other companies frequently in the future. It is more convenient for many things to be done on a business-to-business basis. Youliang is responsible for following up on this matter, mainly to understand the general process first, So as not to turn my eyes black."

"The second is about the catalytic substrate. Qi Huiren called me this afternoon and wanted to send a batch in half a month. They are in a hurry."

"I ordered a batch of instruments online, and they will arrive in a few days. I will send them to Youliang's house first. After I prepare them, I will synthesize a batch for them."

This matter has been discussed with Li Youliang in advance, and it is not suitable to synthesize the catalytic substrate in the scientific research building of the hospital at this time, otherwise it will be very troublesome to explain.

Moreover, the raw materials for synthesizing this catalytic substrate are very simple and can be bought anywhere. The main equipment is several temperature controllers plus centrifuges and chromatographs.

Gao Feng didn't spend a lot of money out of his own pocket. The second-hand equipment he bought was as long as it could be used.

After discussing with several people, they started the previous experiment again. In the experimental procedure provided by the system, the screening in the process of monoclonal antibody preparation was divided into two steps.

First filter:
After cell fusion, the selective cultivation of hybridoma cells is the key to the first screening.The commonly used HAT selective culture medium is to add hypoxanthine (H), aminopterin (A) and thymidine nucleotide (T) to common animal cell culture medium.It is based on the fact that there are two pathways for DNA synthesis in cells:

One pathway is the biosynthetic pathway (the "D pathway"), which synthesizes nucleotides from amino acids and other small molecular compounds, which provide the raw materials for the synthesis of DNA molecules.In this synthesis process, folic acid participates in this process as an important coenzyme, and aminopterin in HAT medium is an antagonist of folic acid, which can block the "D pathway" of DNA synthesis.

Another pathway is the emergency or salvage pathway ("S pathway"), which utilizes hypoxanthine-guanine phosphorylation transferase (HGPRT) and thymidine kinase (TK) to catalyze the transfer of hypoxanthine and thymidine Glycosides generate corresponding nucleotides, and both enzymes are indispensable.Therefore, in the HAT medium, the "D pathway" of unfused effector B cells and the fusion of two effector B cells is blocked by aminopterin, although the "S pathway" is normal, but due to the lack of proliferation in in vitro culture medium Ability, usually about 10d will die.

Second filter:
In the actual immunization process, since the method of continuous injection of antigen is adopted, and an antigenic determinant stimulates the body to form a corresponding effector B lymphocyte, therefore, the mouse spleen is taken out and grown by these single cell clones, and finally selected A hybrid cell line secreting a predetermined specific antibody is produced for expansion culture.

At this point in the experiment, the experiment has basically come to an end. The first screening operation is very simple, and the difficulty lies in the second screening: the hybridoma cells need to be diluted multiple times and seeded on a multi-well cell culture plate so that each well contains One or several hybridoma cells (theoretically, only when the number of cells in 30% of the wells is 0 can there be single cells in some wells).

The main players in this step are Gao Feng and Hu Jiaran, Cheng Song and Li Youliang can only help out, busy until almost 11 o'clock, Gao Feng stopped the operation and asked everyone to go back to rest.

"I guess it will take another two weeks." Gao Feng estimated in his heart, it may be shorter, Cheng Song's level is also improving very quickly, Li Youliang is now in charge of logistics, and he is a little bit weak.
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The next day

It took Gao Feng and Dong Qianqian a long time to finish their ward rounds before they received a call from the bronchoscopy room to let the patient with tracheoesophageal fistula go down yesterday.

When he arrived at the bronchoscopy room, Liang Congyue pulled Gao Feng aside, "Do you know how to do a hard mirror? I haven't seen you do it before, so I don't dare to show off."

"Really, it's actually quite simple." Gao Feng smiled at him, and Liang Congyue asked because he cared about him.

As soon as the anesthetist took the medicine, he turned the patient over, and he quickly inserted his throat and connected it to the ventilator.Gao Feng took an ordinary bronchoscope to look at it first, and took pictures as a routine, which will be used when writing the bronchoscopy report later.

Under the microscope, a fistula with a diameter of about 1 cm can be clearly seen. It is located about 2 cm from the left main bronchus to the crest of the carina, and there are some food residues beside it.

"You can put a Y-shaped stent." The trachea stent material supplier on the side said.

Since the airway stent is a high-value consumable, the Provincial People's Hospital still uses a lot of it. The manufacturer specially assigned an assistant to help when someone puts the stent.

It was the first time for Gao Feng to deal with this kind of person. He was very polite. The young man yelled at each teacher, and his hands and feet were also very quick. It should be because he has been in the bronchoscopy room for a long time, and his concept of asepsis is very good.

However, compared with other surgical operations, bronchoscopy and gastrointestinal endoscopy do not require a particularly high environmental cleanliness. After all, these are cavities where the human body communicates with the outside world, and absolute sterility is not required.

　Brothers ask for a monthly pass.It seems that if you spend 1000, you can draw a lottery to get a recommendation or something.Now the difference is more than 300.

　　
　
(End of this chapter)