This doctor has a system

Chapter 540 603. Special Article

When he arrived at the slaughterhouse, the manager was negotiating with a leader named Wang from the epidemic prevention department.

"They are employees of two factories. Can we just destroy the meat produced by the two factories?"

"That won't work. All existing meat and all unslaughtered animals must be processed in a pollution-free manner." The leader surnamed Wang was very firm in his attitude. He suddenly received the news that anthrax had been discovered and a death order had been issued from above that the matter must be dealt with strictly and quickly.

"But we have more than 500 tons of meat!"

"It doesn't matter. We are not afraid of being tired."

Damn it! The manager almost yelled, am I afraid that you guys are tired?
"I can't make the decision on this. You have to wait for our President Tian to come!"

"It's no use waiting for anyone to come!" The leader surnamed Wang said impatiently. He had received a death order, and he would be severely punished if he failed to complete it. As for whether there would be other problems after the destruction, that would be discussed later.

"Let's see who dares!" As soon as Tian Xingwang arrived, he heard that someone was going to destroy the meat in his factory. This was simply a threat to his life.

One ton of beef costs about 30000 yuan, and there are almost 200 tons in the cold storage, and more than 300 tons of mutton. It would be a disaster if all of it was destroyed!

"The workers in your factory have contracted anthrax." Wang explained to Tian Xingwang for the sake of the people behind him, "The higher-ups have issued a death order, and you, Mr. Tian, ​​must cooperate."

"I'm not cooperating!" Tian Xingwang cursed on the spot, "Do you know how much money I'm going to lose?"

"Almost more than 1000 million!"

"Enough to buy so many of your lives!"

"You." Leader Wang's face flushed instantly. "You are outrageous!"

"Someone come! Close the door for me!"

"Are you looking for death?" Tian Xingwang stepped forward and gave him a big fight, "I'm going to kill you!"

The conflict soon escalated, and under the boss's orders, the workers directly drove out the law enforcement officers.

Leader Wang was shocked and quickly reported the matter to his immediate boss.

"What? How dare he do that!"

A leader who was conducting research in the district soon received a call, and his face became extremely ugly after he hung up.

"idiot!"

Tian Xingwang was quickly arrested. The local authorities immediately closed the slaughterhouse, killed and disposed of animals in surrounding farms, and carried out comprehensive disinfection and monitoring of the surrounding environment.

Fortunately, no other positive livestock were found, and the related livestock products did not enter the market circulation.

At the same time, medical personnel began to carry out comprehensive epidemiological investigation, sampling and testing, and symptom monitoring of people who had contact with sick animals.

The results showed that a total of 6 workers had skin anthrax and only one case of pulmonary anthrax. This result was undoubtedly the most satisfactory, and soon no one paid any attention to the matter.

Fengxin Technology Laboratory

After a busy day at work, Wang Liang stretched and said, "Come on, brothers, let's go out for a drink."

"your treat?"

"Let's split the bill. I'm not that rich." Wang Liang cried poorly in a familiar manner.

Several team members looked at him with disdain and said they were very busy.

"Brother Liang, is it easy for us to get promoted here?" asked a newcomer.

"That's definitely not easy." Wang Liang said casually, "You have to learn to be well-educated and well-mannered."

"Well-educated and well-mannered?" The team member was stunned for a moment, "Team leader, what do you mean? Only by selling your ass can you get promoted?"

"What are you thinking about?" Wang Liang said unhappily, "It's not that simple!"

Group member
"What I mean is that it's not enough to just know the knowledge in the books. You also need to learn how to give gifts." Wang Liang instructed, "Give me some first, and I can recommend you to be the deputy team leader."

"Forget it, I'd better sell my butt." said the team member.

"Wang Liang, do you have any more pipettes?" The team leader of Group 3 came over and said, "Let me take a pack."

"It's already time to get off work, why are you still here to get supplies?" Wang Liang asked curiously, "Are you going to work overtime again?"

"There's nothing we can do. We can't keep up with the progress." The leaders of Group 3 were almost worried to death. They were all at the tail end of the list.

"What stage have you reached?" he asked Wang Liang, "Can it be completed next month?"

"It won't take that long. It should be almost done by next week." Wang Liang replied, "Have you completed 50% of the total?"

The leader of Group 3 looked unhappy when he heard this and he picked up the pipette and was about to leave.

"No way, no way, is there anyone who hasn't even achieved half of the progress?"

"You guys got me drunk last time and said you wanted to compete with me, but I didn't expect you were just bragging. You're messing up your job!"

"Little trash."

"You bastard!" The leader of Group 3 knew that Wang Liang was trying to anger him on purpose, so he turned around and left, not giving the other party a chance to continue.

Hu Jiaran happened to pass by here and heard the whole process of Wang Liang's boasting. "Wang Liang, with your ability, I think you can definitely go out and work on your own."

"I am a member of the company when I am alive and a ghost of the company when I die!"

"Please don't mention such words that are not conducive to unity in the future, Mr. Hu!" Wang Liang said sternly.

"No, why don't you take over the task of Group 3." Hu Jiaran said after thinking for a while. The overall ability of the members of Group 3 is relatively average, and the team leader is slightly better. He was not thoughtful enough when assigning tasks, and now it can be clearly seen that he was forcing things.

"That's not good." Wang Liang was not too averse to taking over tasks from other groups, but he had to consider the feelings of his colleagues.

"Not just their group, the work directions of Group 2 and Group 5 need to be integrated." After Gao Feng came to the laboratory, he accelerated the overall research. Currently, there are still sufficient manpower. Hu Jiaran discussed with several key members and decided to move forward in parallel and achieve results in multiple areas.

"Who is in charge of the CHO cell expression system now?" Gao Feng asked.

"It was 3 groups before, but now it's been assigned to Wang Liang." Yue Penghui at the side quickly replied, "But it seems a bit difficult and the progress is slow."

CHO cells, as mammalian cells, are able to modify and translate the expressed proteins, and their glycoforms are similar to those of human cells. Therefore, the expressed proteins can show similar activity to natural antibodies.

More importantly, CHO cells can grow either adherently or suspended in serum-free medium, and can tolerate high shear stress and osmotic pressure, making it easy to culture CHO cells in large quantities using bioreactors.

The CHO cells that Gao Feng needs to obtain are relatively special and require the introduction of multiple foreign DNA chains. Although there are many methods, each one is not simple.

"You said I couldn't do it, but you're stuck too?" The team leader of Group 3 suddenly felt relaxed. It turned out that it wasn't that he couldn't do it, but that the questions were too difficult. Seeing that Wang Liang was also at a loss, he regained his confidence.

"What do you mean, you little piece of shit?" Wang Liang glared at him. "Do you think I, who was last year's outstanding employee, am not good enough?"

What followed were some difficult-to-understand words, such as "It wasn't like this before", "Maybe I'm not in good shape recently", "I left my phone at home", "Take a break and come back later", etc. The laboratory was filled with a happy atmosphere.

"Cough cough!" Hu Jiaran coughed a few times, and the scene suddenly became quiet.

"Wang Liang, how is the CHO cell cultivation going?" Gao Feng asked.

"We have produced some of it, but the cultivation efficiency is very low and it is not enough for daily experiments." Wang Liang was a little embarrassed. He had boasted a little too much some time ago and suffered a Waterloo in this area.

Wang Liang has tried a number of methods to introduce foreign DNA into mammalian cells, including calcium phosphate, electroporation, cationic lipid-based lipofection, or dendrimer-based methods.

But the results are not very good, and he is trying to find ways to improve it.

Gao Feng looked at the experimental records and felt that Wang Liang did nothing wrong. Currently, liposome transfection and electroporation are still the most common introduction methods.

Developing and optimizing cell lines requires a lot of manpower and time. Transfection methods can only be used to construct cell lines that transiently express monoclonal antibodies.

In order to obtain cell lines stably transfected with exogenous plasmids, multiple technologies are needed to screen cell lines. At the same time, this process will further screen out cell lines with high expression levels.

"Did you write this?" Gao Feng couldn't help but look up at Wang Liang. The improved method for transfection was very feasible and was almost the same as what he saw in the system space.

"Yes, the success rate will be a little higher, but the difference compared to before is not that big." Wang Liang was a little depressed when talking about this. This took him a lot of thought, but the result was a bit disappointing.

"Adjust the parameters. The temperature is wrong. The most suitable reaction temperature for the enzyme you added is 56°C. However, a large amount of heat is released during the substrate reaction. The temperature of the solution is probably at least 80°C." Gao Feng pointed out the problem.

"Keep up the good work! I feel like you're likely to be the outstanding employee this year."

"Okay, Mr. Gao!" Wang Liang was immediately excited.

After Gao Feng left, he started to show off again.

"Comrades, as long as you work as hard as I do and make achievements, you will definitely get a salary increase, if not a promotion."

"Maybe one day a new group will be formed and you will be the leader."

This is just another empty promise. Several old employees were choked and couldn't breathe. The taste was too strong and it was really hard to eat! I couldn't finish it. I couldn't finish it at all!
But the eyes of several young new employees began to light up, and they looked like they were still hungry and not full.

"You three should work overtime today. The boss might be here tonight." Wang Liang began to deceive the newcomers. He has grown into a qualified little leader.

But Wang Liang was right. Gao Feng did not leave tonight. He was reviewing manuscripts.

This article is about hereditary spastic paraplegia type 50, a rare, chronic disease in which patients gradually lose their motor skills due to spasticity, become disabled, suffer severe cognitive impairment, and often do not survive into adulthood.

At present, there are only 80 patients known to be affected worldwide, and there are 224 countries and regions in the world.

The author of the article is Professor Lasse West of Cornell University Medical School, a top professor of neurology.

This article is also quite special because it is actually a record of clinical trials of a patient suffering from hereditary spastic paraplegia type 50, and there is only one patient.

Parton was born two years ago and is the only son in the family. However, before the Parton family could celebrate his arrival, bad news came one after another.

First, it was Parton's grandmother who sensed something was wrong. She was a nurse at a private hospital.

She found that Patton's developmental milestones were significantly behind the normal level. At the age of 5 months, he still couldn't lift his head or raise his hands. The family even found that the little guy's fingers always seemed to be weak when grasping.

Driven by a strong sense of unease, Parton's family began to take him to seek medical treatment. A neurological specialist physical examination found that Parton's muscle tension was reduced, and a cranial MRI showed abnormal signals in the thin corpus callosum and white matter.

And shortly afterwards, Parton suffered a major epileptic seizure, which undoubtedly cast a deeper shadow on his family's hearts.

When Patton was one year and five months old, genetic testing showed that he had a compound heterozygous mutation in the AP4M1 gene, which resulted in the loss of function of the adaptor protein complex 4 encoded by the gene.

The disease caused by this gene mutation is called hereditary spastic paraplegia type 50 (SPG50). Symptoms of this disease usually appear in infancy and include global developmental delay, progressive microcephaly and abnormal brain magnetic resonance imaging.

"Hey guys, you guys are very young, why don't you have another child?" the doctor suggested.

"Then what should he do?"

"Maybe he came into this world too hastily, or maybe he wasn't prepared enough."

Patton's parents were unwilling to give up. They tried every possible way to find a chance of treatment: communicating with the families of patients with similar rare diseases (SGP47, SPG15, CMT4J, etc.), reading medical literature, and attending academic conferences...

Patton's father attends almost every gene and cell therapy conference. He prints information about his son's disease into pamphlets and distributes them everywhere, looking for experts who might understand SPG50.

But most of the time there is no gain, and some people don't even open it to take a closer look.

But hard work pays off. Professor Lasse West of Cornell University Medical School accidentally learned about this situation.

He told Patton's parents that they could try to repair Patton's damaged genes through gene editing. But the difficulty is that gene therapy for SPG50 has not yet been invented, and due to its one-to-one customization characteristics, research and development requires at least $400 million.

Without any hesitation, the Patton family immediately sold all their property. But it was obvious that the family savings alone were far from enough.

Professor Lasse West gave them enough support in the future, and he spared no effort to help this poor child. Due to his huge influence, many institutions began to contact Parton's family.

Soon, the funds needed for research and development were raised, and related research progressed rapidly.

(End of this chapter)