This doctor has a system
Chapter 98 Supraventricular Tachycardia
Chapter 98 98. Supraventricular Tachycardia
"It seems to make sense, then you can directly vote for nature." Li Youliang smiled.
"I'm afraid it won't work naturally." Gao Feng still had self-knowledge, "I'll vote for Nature Medicine, a sub-journal of Nature."
"I was just joking, you take it seriously." Li Youliang said speechlessly, "You should vote for INT J MOL ME, the possibility of publication is higher, after all, you are the second submission."
"What you said makes sense. I've decided to invest in Nature Medicine." Gao Feng made a decision.
Li Youliang.
"Just wait until you get a bloody head." He shook his head.
After returning home, Gao Feng also told Li Xinyao about submitting the manuscript, but Li Xinyao did not laugh at him, "Submit, I want to witness the rise of Academician Gao in the future."
"Well said." Gao Feng turned on the computer, directly found the email address of Nature Medicine, and clicked send.
Fu Jie's cousin came to the hospital for reexamination, and his father specially sent a pennant to Dong Qianqian and Gao Feng, and Gao Feng gained a skill point from him.
As noon approached, Fu Xinxin's family insisted on inviting everyone to dinner, and they waited in the office. Dong Qianqian and Gao Feng had no choice but to go down together.
"Let's go eat Haidilao." Dong Qianqian suggested, "It's close, and it's still lively." No one objected.
"There are basically no symptoms now." Fu Xinxin said, "I have regained my life."
"You have received Fu Xinxin's sincere thanks, and you have obtained a skill point."
Gao Feng was overjoyed, Fu Xinxin's family really got along.
-
Xiong Huaxin stayed in BJ for a while and then returned to State Z.Li Youliang drove to the airport to pick him up, "Mr. Xiong, why do you look so haggard?"
"Can you not be haggard?" Xiong Huaxin lay comfortably on the passenger seat, and Li Youliang adjusted the distance between the seats for him.
"I was busy summarizing the experimental data when I got back, and worked overtime for a few days." Xiong Huaxin took two sips of the purified water handed over by Li Youliang, "I'm almost exhausted."
"It's not easy." Li Youliang sighed.
Xiong Huaxin asked about the situation here, and Li Youliang told him that there were no major changes.Business as usual.
"For our project, Gao Feng wrote an article and is going to send it out." He said, "There will be an inspection team from the province in a few days, saying it will help us solve the problem."
"What problem to solve? Do we have any problems now?" Xiong Huaxin asked.
"If there is no problem, you have to create a problem, or people will come here for nothing." Li Youliang smiled, "It has to be a problem that can be solved in a short period of time. Leaders don't like it if it takes too long."
"It can't be too big a problem, or the person who raised the problem will be easily solved."
"It's best to have some issues that are not big or small, and that can reflect the leadership's concern for us."
Xiong Huaxin looked at Li Youliang for a long time, "You are so fucking talented! Who did you learn from?"
Li Youliang was stunned for a moment, "Aren't these all common human ways of the world? Everyone should know it?!"
"Youliang, come with me to BJ when the project here is finished, I will not treat you badly." Xiong Huaxin said, "I will settle your account for you, and within 10 years, you will be guaranteed to buy a house in the urban area over there."
"Mr. Xiong, you are flattering me too much." Li Youliang was a little moved in his heart.
What I did tonight was the purification of monoclonal antibodies. This step is relatively simple and easy for everyone.
First, centrifuge the ascites at 4 rpm for 12000 min at 15°C to remove impurities.
Then take 1 part of ascitic fluid and add 2 parts of 0.06mol/L PH5.0 acetate buffer solution, add 33ul octanoic acid per ml of diluted ascitic fluid, add octanoic acid drop by drop under stirring at room temperature, and mix at room temperature for 30 min
Stand at 4°C for 2 hours, take out 12000 g and centrifuge for 30 minutes, discard the precipitate;
The supernatant was filtered through a nylon sieve, and dialyzed in 50 times the volume of 0.01MPH7.4 PBS at 4°C.
An equal volume of saturated ammonium sulfate solution was added to the dialyzed supernatant.
Finally, take a small amount of dialyzed sample and dilute it properly, then use a UV spectrophotometer to detect the protein content, SDS-PAGE, and WB to detect the purity of the antibody.
Since dialysis takes about 6 hours, everyone went back after being busy at this step, and did the rest the next day.
In the morning, Gao Feng and Dong Qianqian quickly finished checking the room, "I feel so much more relaxed after you came!" Dong Qianqian was very happy. Although he was in the same team as Shi Peiyuan before, but the other party was unreliable, she did not dare to entrust the patient to The opponent handles it.
After Gao Feng came, the two could take turns to rest every week, and felt much more relaxed.
"Please allow me to fall to the ground." Gao Feng's cell phone rang.
"Hello."
"Is it Gao Feng? I'm your second aunt." A slightly rough voice came over.
"Second Aunt?" Gao Feng was stunned for a moment, and there seemed to be no impression in his mind, "What's the matter with you?"
"It's like this. Isn't your sister pregnant? The doctor at the hospital here said it was an ectopic pregnancy." The second aunt said, "They also said that it will be removed by surgery."
"I thought it was not easy to get pregnant. Your sister and your brother-in-law have been tossing in bed for more than a year."
"See if you can move the child from outside the palace to inside the palace." The second aunt said to Gao Feng, "The big hospital over there must be of a higher standard."
Gao Feng was stunned, you think this is moving, just live in another place!
"This won't work, the current technical level has not reached this level yet."
"What, I was still thinking about what you can do." The second aunt was very disappointed, "Then you should be busy." She hung up the phone neatly.
Gao Feng couldn't laugh or cry when he heard the beeping sound of the mobile phone.
He checked the relevant treatment online again, and the current technical level is indeed not at this stage.
It seems that I can only let my sister and brother-in-law who are out of reach continue to toss on the bed——
When getting off work, a nurse came and called Gao Feng, "Go and see bed 28, the patient will say that he is flustered."
Bed 28 is an old man with chronic obstructive pulmonary disease, named Gou Jianshe. This time, he suffered an acute exacerbation of COPD due to exposure to rain and cold, and he has remained stable for the past two days.
Gao Feng didn't dare to be careless. The elderly's physical function is very poor, and various unexpected accidents may occur, especially heart disease.
"Master Gou, what's the matter with you?"
Gou Jianshe was a little nervous, "I just ate an apple, and now I feel my heart beating all the time, and I feel very flustered."
"Tianci, go and push the electrocardiogram machine over."
Gao Feng took the stethoscope and listened, good guy, the heart rate must be 180 beats per minute (normal value 60-100), but the heart rate is quite regular, it should not be atrial fibrillation.
Is it supraventricular tachycardia?Gao Feng thought to himself.
Zhang Tianci quickly took an electrocardiogram for the patient, Gao Feng looked at it, and it was indeed supraventricular tachycardia.
"Has this happened before?" Gao Feng asked.
"It happened a few times, and each time was not long." Gou Jianshe thought about it for a while, "Washing your face every time will be fine."
"Heavenly gift, you use a basin to fetch cold water for the patient and let him wash his face." Gao Feng confessed.
Zhang Tianci froze for a moment, then ran to fetch water.
(End of this chapter)
"It seems to make sense, then you can directly vote for nature." Li Youliang smiled.
"I'm afraid it won't work naturally." Gao Feng still had self-knowledge, "I'll vote for Nature Medicine, a sub-journal of Nature."
"I was just joking, you take it seriously." Li Youliang said speechlessly, "You should vote for INT J MOL ME, the possibility of publication is higher, after all, you are the second submission."
"What you said makes sense. I've decided to invest in Nature Medicine." Gao Feng made a decision.
Li Youliang.
"Just wait until you get a bloody head." He shook his head.
After returning home, Gao Feng also told Li Xinyao about submitting the manuscript, but Li Xinyao did not laugh at him, "Submit, I want to witness the rise of Academician Gao in the future."
"Well said." Gao Feng turned on the computer, directly found the email address of Nature Medicine, and clicked send.
Fu Jie's cousin came to the hospital for reexamination, and his father specially sent a pennant to Dong Qianqian and Gao Feng, and Gao Feng gained a skill point from him.
As noon approached, Fu Xinxin's family insisted on inviting everyone to dinner, and they waited in the office. Dong Qianqian and Gao Feng had no choice but to go down together.
"Let's go eat Haidilao." Dong Qianqian suggested, "It's close, and it's still lively." No one objected.
"There are basically no symptoms now." Fu Xinxin said, "I have regained my life."
"You have received Fu Xinxin's sincere thanks, and you have obtained a skill point."
Gao Feng was overjoyed, Fu Xinxin's family really got along.
-
Xiong Huaxin stayed in BJ for a while and then returned to State Z.Li Youliang drove to the airport to pick him up, "Mr. Xiong, why do you look so haggard?"
"Can you not be haggard?" Xiong Huaxin lay comfortably on the passenger seat, and Li Youliang adjusted the distance between the seats for him.
"I was busy summarizing the experimental data when I got back, and worked overtime for a few days." Xiong Huaxin took two sips of the purified water handed over by Li Youliang, "I'm almost exhausted."
"It's not easy." Li Youliang sighed.
Xiong Huaxin asked about the situation here, and Li Youliang told him that there were no major changes.Business as usual.
"For our project, Gao Feng wrote an article and is going to send it out." He said, "There will be an inspection team from the province in a few days, saying it will help us solve the problem."
"What problem to solve? Do we have any problems now?" Xiong Huaxin asked.
"If there is no problem, you have to create a problem, or people will come here for nothing." Li Youliang smiled, "It has to be a problem that can be solved in a short period of time. Leaders don't like it if it takes too long."
"It can't be too big a problem, or the person who raised the problem will be easily solved."
"It's best to have some issues that are not big or small, and that can reflect the leadership's concern for us."
Xiong Huaxin looked at Li Youliang for a long time, "You are so fucking talented! Who did you learn from?"
Li Youliang was stunned for a moment, "Aren't these all common human ways of the world? Everyone should know it?!"
"Youliang, come with me to BJ when the project here is finished, I will not treat you badly." Xiong Huaxin said, "I will settle your account for you, and within 10 years, you will be guaranteed to buy a house in the urban area over there."
"Mr. Xiong, you are flattering me too much." Li Youliang was a little moved in his heart.
What I did tonight was the purification of monoclonal antibodies. This step is relatively simple and easy for everyone.
First, centrifuge the ascites at 4 rpm for 12000 min at 15°C to remove impurities.
Then take 1 part of ascitic fluid and add 2 parts of 0.06mol/L PH5.0 acetate buffer solution, add 33ul octanoic acid per ml of diluted ascitic fluid, add octanoic acid drop by drop under stirring at room temperature, and mix at room temperature for 30 min
Stand at 4°C for 2 hours, take out 12000 g and centrifuge for 30 minutes, discard the precipitate;
The supernatant was filtered through a nylon sieve, and dialyzed in 50 times the volume of 0.01MPH7.4 PBS at 4°C.
An equal volume of saturated ammonium sulfate solution was added to the dialyzed supernatant.
Finally, take a small amount of dialyzed sample and dilute it properly, then use a UV spectrophotometer to detect the protein content, SDS-PAGE, and WB to detect the purity of the antibody.
Since dialysis takes about 6 hours, everyone went back after being busy at this step, and did the rest the next day.
In the morning, Gao Feng and Dong Qianqian quickly finished checking the room, "I feel so much more relaxed after you came!" Dong Qianqian was very happy. Although he was in the same team as Shi Peiyuan before, but the other party was unreliable, she did not dare to entrust the patient to The opponent handles it.
After Gao Feng came, the two could take turns to rest every week, and felt much more relaxed.
"Please allow me to fall to the ground." Gao Feng's cell phone rang.
"Hello."
"Is it Gao Feng? I'm your second aunt." A slightly rough voice came over.
"Second Aunt?" Gao Feng was stunned for a moment, and there seemed to be no impression in his mind, "What's the matter with you?"
"It's like this. Isn't your sister pregnant? The doctor at the hospital here said it was an ectopic pregnancy." The second aunt said, "They also said that it will be removed by surgery."
"I thought it was not easy to get pregnant. Your sister and your brother-in-law have been tossing in bed for more than a year."
"See if you can move the child from outside the palace to inside the palace." The second aunt said to Gao Feng, "The big hospital over there must be of a higher standard."
Gao Feng was stunned, you think this is moving, just live in another place!
"This won't work, the current technical level has not reached this level yet."
"What, I was still thinking about what you can do." The second aunt was very disappointed, "Then you should be busy." She hung up the phone neatly.
Gao Feng couldn't laugh or cry when he heard the beeping sound of the mobile phone.
He checked the relevant treatment online again, and the current technical level is indeed not at this stage.
It seems that I can only let my sister and brother-in-law who are out of reach continue to toss on the bed——
When getting off work, a nurse came and called Gao Feng, "Go and see bed 28, the patient will say that he is flustered."
Bed 28 is an old man with chronic obstructive pulmonary disease, named Gou Jianshe. This time, he suffered an acute exacerbation of COPD due to exposure to rain and cold, and he has remained stable for the past two days.
Gao Feng didn't dare to be careless. The elderly's physical function is very poor, and various unexpected accidents may occur, especially heart disease.
"Master Gou, what's the matter with you?"
Gou Jianshe was a little nervous, "I just ate an apple, and now I feel my heart beating all the time, and I feel very flustered."
"Tianci, go and push the electrocardiogram machine over."
Gao Feng took the stethoscope and listened, good guy, the heart rate must be 180 beats per minute (normal value 60-100), but the heart rate is quite regular, it should not be atrial fibrillation.
Is it supraventricular tachycardia?Gao Feng thought to himself.
Zhang Tianci quickly took an electrocardiogram for the patient, Gao Feng looked at it, and it was indeed supraventricular tachycardia.
"Has this happened before?" Gao Feng asked.
"It happened a few times, and each time was not long." Gou Jianshe thought about it for a while, "Washing your face every time will be fine."
"Heavenly gift, you use a basin to fetch cold water for the patient and let him wash his face." Gao Feng confessed.
Zhang Tianci froze for a moment, then ran to fetch water.
(End of this chapter)
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