medical road with you

Chapter 82 Experiment
After confirming that everything was normal for adults and children, Lu Ping and Tianhai returned to Cuihu Community.

Hanging the coat on the hanger, Lu Ping tilted his body and fell into the sofa. His arms rested on the armrest of the sofa and his chin was dragged. Lu Ping stared at the TV in a daze.

Tianhai poured Lu Ping a glass of warm water: "Pingping, what's wrong?"

"Tianhai, do you want a child?" Lu Ping asked suddenly.

Tianhai pretended to smile lightly: "Why do you think of asking this again? Didn't we agree before?"

Lu Ping was extremely stubborn, and asked again: "Tianhai, do you want a child?"

Tianhai paused slightly, put the water glass on the coffee table, sat down next to Lu Ping, and patted her head: "It seems that Chi Na's childbirth has had a big impact on you, you're starting to think about it Be good, go to sleep, tomorrow, you will be fine."

Just as Tianhai was about to get up, Lu Ping took his hand: "Tianhai, I want to give you a baby."

Tianhai paused, turned a few times like clockwork, and turned to Lu Ping, with somewhat stunned, puzzled, and uncertain eyes.

Lu Ping looked into those eyes: "I want to give you a baby, so don't worry about my graduate student, answer me honestly, do you want a baby?"

Tianhai suddenly pulled Lu Ping into his arms, and hugged her hard, as if wishing to embed her in his body: "I want to, of course I want to, why wouldn't I want to?"

Three consecutive 'thoughts' made Lu Ping understand how much Tianhai longed for a child that belonged to her and him. If Lu Ping didn't still have a sliver of reason, she would definitely drag Tianhai to make a child now.

After a while, Tianhai regained his composure, and let go of Lu Ping: "But, now is not the time to have a child. I'm not in a hurry. We will have plenty of opportunities in the future."

"kindness!"

Lu Ping suddenly thought of Zhou Feng's nervous appearance again, and couldn't help but smile: "That Zhou Feng, who usually looks quite stable, is really messed up today, he is too nervous."

Tianhai stared at Lu Ping: "Do you think Zhou Feng is nervous today?"

"Isn't that tense enough?"

Tianhai's eyes became serious: "If it were me, if I knew that you were going to give birth to me soon, I would definitely be a hundred times more nervous than him!"

Lu Ping:. . .

**
In the first semester of graduate students, the main purpose is to complete the required courses to earn credits. In the second semester, the course study is much less, and most of the time is spent in the laboratory.

The most important task in the second semester is to choose topics and develop laboratory skills at the same time.

The most commonly used skills in virology laboratories are plasmid DNA extraction, cell culture, PCR, and of course, some general skills, such as tissue section making and staining.

And Lu Ping stayed in Song Tianwen's laboratory for three months, and did one thing: PCR, that is, gene amplification.

"Cultivate more basic skills. This is the most important thing. As for the topic selection, you usually read more literature, and come to me for discussion if you have your own attention."

After Song Tianwen threw such a sentence to Lu Ping, he basically disappeared.

It was a senior who taught Lu Ping how to do the PCR experiment. After explaining a bunch of terms expressionlessly, the senior asked coldly, "Do you understand the operation steps and points to pay attention to?"

"Understood!"

Lu Ping's memory is very good, the senior brother said it again, she did remember it.

"It's good to understand, and you can do the rest."

After the senior brother finished speaking, he pushed a bunch of samples to Lu Ping, and then left the laboratory.

Lu Ping thought he was going to the bathroom to smoke a cigarette or something, but he waited and waited for half an hour before the person came back. Lu Ping finally realized that he wanted her to finish the rest of the samples by herself.

Because it is to amplify the specified DNA fragment, the primers are already prepared, Lu Ping does not need to design the primers and just follow the experimental steps.

Lu Ping also did some experiments during his university days, but he just killed a small white mouse or a small frog, took some reagents and poured them here and there. Lu Ping had never done such a high-level experiment.

Looking at the lab coat on his body, Lu Ping couldn't help feeling a little nervous.

Picking up the micro-volume adjustable pipette, Lu Ping's hands trembled a little.In the past, the test solution was taken in 10ml and 20ml in the experiment, and the ear washing ball or glass straw was used. Now, the test solution dosage is in ul. The difference is a thousand times, but fortunately, there is a special one. pipette.

Lu Ping has seen the micro-volume adjustable pipette in movies and documentaries before, and always thought it was very high-end. Today, he finally used it.

After repeatedly confirming what is the first gear and what is the second gear, Lu Ping finally made up his mind to get the first tube of test solution.The senior brother said that there is a specific requirement for the order in which the test solutions are put in. After thinking about it, Lu Ping decided to use ddH2o (double distilled water) to practice his hands. There are many reagents in this laboratory, so it doesn't matter if you spill some.

After adjusting the volume to 10ul, Lu Ping inserted the tip into ddH2o, pressed the second gear to absorb liquid, carefully moved the pipette to the microcentrifuge tube, and pressed the second gear to release the liquid, but Lu Ping pressed it for a long time, and still there was no trace Liquid comes out.

Could it be that he didn't get it just now? Lu Ping reinserted the suction head into the ddH2o, made sure that the shampoo was under the liquid, sucked it again, and drained the liquid again, but there was still nothing.

What a ghost, she can't do a simple thing like taking the liquid.

Either she is doing something wrong, or there is something wrong with this pipette.Lu Ping looked around and found a row of pipettes in a glass cabinet. He chose one with the same volume. Lu Ping tried again, but the result was still the same.

It seems that there is indeed a problem with my operation, but she is the only one in the laboratory, and she wants to find someone to ask the reason, but there is no reason.

Du Niang, if there is a problem, find Du Niang, this is the first reaction of modern youth.

Just as Lu Ping was about to take out his mobile phone, he saw the gloves on his hands, and then looked at his lab coat, and remembered what his senior brother said: PCR is most afraid of pollution, and false positives are prone to occur.

After careful consideration, Lu Ping took off his lab coat and gloves, and left the laboratory.

After leaving the laboratory and standing in the corridor, Lu Ping took out his mobile phone.

It was very cold in February, and there was a gust of wind blowing in the corridor. Lu Ping sneezed because he didn't wear much.

However, Lu Ping didn't notice all this at all. She immediately opened Du Niang and searched for micro-volume adjustable pipettes.

Staring at the precautions one two three four five six seven, Lu Ping suddenly realized that there are so many things to pay attention to when using a pipette.

Keeping all precautions firmly in mind, Lu Ping returned to the laboratory.

This time, it finally succeeded.

Lu Ping smiled with satisfaction, and those who didn't know thought she had done a great thing.

Take all the reaction reagents, put the centrifuge tube into the PCR machine, Lu Ping set up the PCR reaction program according to the brother's instructions, pre-denaturation, denaturation, extension cycle 30 times, annealing.

The whole process takes about 2 and a half hours.

Staring at the PCR instrument for half an hour, listening to it rotate at high speed and vibrate slightly for a while, Lu Ping realized that he should not stare at the instrument so stupidly. After the gene amplification, it needs to be tested by agarose gel electrophoresis Quantitative.

Brother said the process of making glue is quite simple, but who knows.

Lu Ping thought about it and decided to ask Du Niang for help again.

After taking off his lab coat, Lu Ping went out of the lab again.

What is very simple, brother is talking nonsense, there are 4 big steps, a bunch of small steps, and there are various precautions.

After studying carefully for nearly an hour, Lu Ping finally memorized the detailed steps.

Back in the laboratory, Lu Ping put aside the process in his mind, but it was still too abstract, it was all words.

Lu Ping thought for a while, walked to the glue maker, and carefully checked all the equipment used. This time, the process was much clearer.

Beep, beep, a series of warning tones, Lu Ping responded after two seconds, and the PCR was completed.

Taking out the centrifuge tube, Lu Ping began to operate it carefully while recited the procedure that he had memorized several times in his heart.

Gel preparation, sample mixing, spotting, electrophoresis, every step Lu Ping checked again and again that nothing went wrong.

After plugging in the electrodes and pressing the power button, Lu Ping stared at the DNA spectrometer, and finally, after 10 minutes, a short, somewhat fuzzy yellow-green fluorescence appeared.

"Ah, I did it, I did it!"

After bouncing on the spot three times, Lu Ping stared at the fluorescent light again and again, covering his mouth that couldn't close his smile. Finally, unable to hold back the joy in his heart, Lu Ping stood on tiptoe and flew into the air , turned around and did a ballet spin.

This turn, only turned 180 degrees, and the person stopped.

Outside the window, Tianhai was wearing a windbreaker with his hands in his pockets, looking at her with a cloudy face.

"Honey, why are you here?" Through the glass, Lu Ping shouted to Tianhai outside the window.

Tianhai didn't seem to hear her voice, and still looked at her gloomyly.

This laboratory is completely sealed, and the sound insulation effect is very good, Lu Ping finally remembered.

Lu Ping took off his gloves and lab coat, and ran out.

"Honey, why are you here?" Lu Ping asked with a smile.

After staring at Lu Ping for three seconds, Tianhai's face became more and more ugly.

Pursing his lips and saying nothing, Tianhai tore off the windbreaker on his body, grabbed the collar, walked around Lu Ping's head, and threw it hard, the big windbreaker wrapped Lu Ping tightly.

"Husband, what's wrong?"

Lu Ping finally noticed Tianhai's unhappiness, and pretended to be aggrieved.

Raising his wrist and pulling out his watch, Tianhai knocked on the dial three times: "Look, what time is it now?"

Lu Ping leaned over: "It's only eleven o'clock!"

After speaking, Lu Ping felt that something was wrong. She started the experiment at ten o'clock, how could it be only eleven o'clock.

No, it won't be eleven o'clock in the evening.

Lu Ping looked up guiltily, and the darkness outside confirmed her terrible conjecture.

Broken, made a big mistake, Lu Ping immediately lowered his head, assuming an attitude of admitting his mistake.

Tianhai repeatedly told her many times that if she couldn't go home on time, she must tell him.Her normal time to go home is six o'clock, and it is already eleven o'clock now, that is to say, she was late for a full five hours!

Tianhai had no time to figure out how late she was with her, so his attention was shifted to the phrase 'it's only eleven o'clock'.

"You didn't even eat lunch, did you?"

At this time, Tianhai is already a group of black clouds before the storm.

"I..."

Lu Ping didn't dare to say no, for fear that his "no" would trigger thunder and lightning in the black cloud in front of him.

"you..."

Tianhai couldn't say anything, he was angry, anxious and distressed.

"Go, come home with me."

Hugging Lu Ping's calf and lifting it upwards, Tianhai directly held Lu Ping on his shoulders.

"Hey, hey, wait a minute, I haven't turned off the lights yet."

Tianhai patted Lu Ping's butt: "The college is not short of the electricity bill."

Back home, Tianhai put Lu Ping on the chair by the dining table: "You wait here."

After speaking, Tianhai turned and went to the kitchen.

kitchen?How does he want to punish me this time, rolling pin?

As a result, 2 minutes later, Tian Hai came out with food: "Eat quickly!"

His tone was still blunt, but it didn't seem so scary anymore.

Lu Ping looked at the stewed radish with braised pork and asked in surprise, "You made it?"

Tianhai hummed, with a bit of sullen expression on his face, and looked at Lu Ping ferociously.

Lu Ping was most afraid of Tianhai's light of judgment, so he swallowed his flattering words, and quickly bowed his head to eat.

He was really hungry too, Lu Ping cleaned up all the food in 3 minutes.

"Are you full?" Tianhai's voice sounded like a teacher was lecturing a student.

"Yeah." Lu Ping raised his head carefully, and when he raised his head halfway, he quickly lowered his head to meet Tianhai's fierce eyes.

Well, I'm full, so he can safely punish me, what new tricks will he use?
Lu Ping was still thinking about it, so Tianhai asked coldly, "Why didn't you reply to my message?"

"what news?"

Lu Ping immediately took out his phone and pressed the power button, but the screen was still black.

Oops, the phone is off.It should be that she used too much electricity to check the information, and it automatically shut down.

The phone was turned off, and he couldn't find her by calling. This was another big taboo of his.

It's too bad, I don't know if I can make it through tonight.

Tianhai looked at the black screen and helplessly raised his forehead. Forget it, what's his rivalry with her? It's not the first day he knows her. Doesn't he understand her temperament?

"Okay, go to sleep." Tianhai's voice was a little tired.

"What? Sleep!" Lu Ping suddenly raised his head and looked at Tianhai in astonishment.

Tianhai nodded her forehead: "What? Don't go to bed, do you want to do the dishes?"

If Tianhai's tone hadn't returned to his usual pampering, Lu Ping would have said loudly: "Honey, I really know I was wrong, come as soon as you want to punish me, don't hang on to me, I'm so scared ah!"

Lu Ping still obediently went to bed, but as soon as he lay down, Lu Ping became nervous again.

The bed, that is the most favorable place for him to punish her.

After a while, Tianhai also came up and held her in his arms as usual.

Lu Ping's breathing became tense, and she stood up like a zombie, waiting silently!
However, a few minutes passed, and Tianhai didn't make any move.

Could it be that what happened today is over?

Lu Ping began to have this fantasy.

Five minutes later, Lu Ping confirmed that this was not a fantasy, but a fact, Tianhai really didn't pursue today's matter.

For some reason, Lu Ping felt very, very guilty. This kind of guilt was worse than Tianhai scolding her severely.

"Honey, I'm sorry!" Lu Ping whispered in the dark.

Tianhai didn't know where her laboratory was. He was so anxious at the time that he found her one by one from room to room.

He was so anxious, but when he found her, he didn't knock on the door immediately, but waited until she finished the experiment.

He must be very worried if he doesn't reply to his messages or answer his calls.

Once, Lu Ping came home half an hour late because he didn't hear his call because the phone was on silent, and he trained her for an hour as soon as she came home.

This time, she was so late for so long, he must be very angry and worried.

"Okay, it's alright, go to sleep."

Tianhai sighed in his heart, and hugged Lu Ping even tighter.

What else could he do? Apart from silently supporting her and taking good care of her, there was really nothing he could do.

In fact, how much he hopes that she is an ordinary girl, a flower, a box of chocolates, and a few nice words can make her feel very happy, but he knows that she is not an ordinary person, and the price of her happiness is more than Much more expensive.

If he could, he really wanted to lock her in his sight, so that he wouldn't have to think about her and worry about her all the time.

However, he knew that she was a sunflower, yearning for sunshine, yearning for freedom and yearning for her own dreams.

So, he really can't do anything, except silently support her and take good care of her.

(End of this chapter)