There is no need to be too normal to be a doctor.

Chapter 93 He actually tried to... (score report)

It took Chen Gong less than 30 minutes to complete all the test papers.

The rest of the time, he was thinking about one thing...

loss!

I paid for this exam myself.

What I originally hoped to see was a group of top biochemistry experts who stayed up all night, spent a lot of energy, and racked their brains to design exciting topics.

Then conduct a cultural game at the ideological level.

Finally, in this game, I discovered my true self, checked for omissions and filled in the gaps, and improved myself.

This is the real effect an exam should have!
It is also the most romantic story in Chen Gong's mind.

But... I never thought that after coming here, I would do the questions of my own design? !
I had a left-right fight with myself.

Of course, in fact, none of the questions are the same as Chen Gong’s simulation questions.

But at Chen Gong's level, can't you see that this is a way of thinking?
In this set of questions, most of the questions are based on your own ideas and perspectives, especially in terms of inducing the development of biochemical scientific research.

Although it is undeniable…

The person who asked the question is really quite good, but... no matter how good he is, he can't escape Chen Gong's thinking mode when he asked the question.

Therefore, this time's written test... for Chen Gong, there was really no gain.

He didn't feel the joy of playing against a master!

so that...

Besides getting a useless high score, what else is it good for?

Life!
Especially studying, it is never about an illusory score of 100 points.

This kind of low-level taste made Chen Gong tired of 100% happiness when he was in the fourth grade of elementary school.

What he wants is the kind of hearty thinking game and dispute!

But now...

What I saw was still a 100-point test paper that was full of boring, boring, boring...

It's really a big loss!

How does Chen Gong think and lose...

"Hello, I'll hand in the paper!"

"Sorry, you can't hand in the paper in advance before the end of the exam."

The invigilator interrupted Chen Gong's unrealistic fantasy and destroyed the child's last chance to escape.

Chen Gong took a deep breath and looked around.

Looking at everyone's sad faces, watching everyone calculating every question and thinking about every detail with serious expressions.

Chen Gong is really envious!
perhaps……

They are much happier than themselves, at least they can see a wonderful test paper.

Hey……

Chen Gong sighed and looked out the window. Maybe only Bai Yun could understand what he meant at this time?

Chen Gong was so bored that he could only close his eyes and start simulating the afternoon skill assessment.

After all, he had no interest in checking such a topic.

A second glance shows disrespect for one's own professional knowledge.

However, Chen Gong thinks, is it because this assessment is too low-level?
Would those high-level assessments be any better?
Thinking of this, Chen Gong was a little moved.

……

I finally made it to the end of the exam.

Chen Gong stood up anxiously, left the examination room, and drove to the anatomy examination center.

When I was going out, I happened to meet Yang Li.

It's just that... the other person's eyebrows were tightened, and he looked a little sad.

Seeing this scene, Chen Gong couldn't help but feel envious.

Yang Li saw Chen Gong and said hello: "Chen Gong, how did you do in the exam?"

Chen Gong sighed and shook his head, how about it?Extremely disappointed!

Yang Li was helpless: "I've also heard that this assessment is a new model of examination questions that our domestic experts in the field of biochemistry personally conducted."

"I... didn't care at first!"

"However, I didn't expect the difficulty to increase so much."

"It's not just you and me, it's also a lot of people. Look...most of these people don't get used to it."

"It must be difficult!"

"However, these questions are really interesting. I am very happy doing them. I even feel like I can improve by doing them."

Chen Gong looked at Yang Li and said seriously: "Instructor, I really envy you!"

Yang Li was stunned for a moment, then smiled: "Why do you envy me? Oh... Actually, my mentality has always been very good."

"Besides, the problem is difficult for everyone, not just one person."

"I can only say that I am not so sure in my heart, so I am worried."

"Actually, don't worry. You are still young. You are only a sophomore. How many are better than you?"

Yang Li thought Chen Gong was feeling uncomfortable because he didn't do well in the exam, so she opened her mouth to comfort Chen Gong.

Then, Chen Gong suddenly said: "I really envy you. You can encounter difficulties in the exam, find your own shortcomings, and compare with the questions."

"Hey……"

"I can't do it!"

"For me, apart from giving me a boring and useless 100 points, this test question does not do much for judging my personal ability, testing my shortcomings, or even improving my overall strength. usefulness."

"Hey, I really envy you. If I were like you and got such a low score, I would be very happy."

After saying that, Chen Gong shook his head and fiddled with the car keys in his hand: "I have to go to the next examination room."

"Goodbye, instructor!"

"I hope I can encounter some questions that I don't know in the next exam!"

After saying that, Chen Gong stepped on the accelerator, and the tram started and moved away in an instant.

Yang Li was left standing in the wind, messy for a long time, and she didn't come back to her senses for a long time. What does it mean...

What does it mean... "Hey, I really envy you. If I were like you and got such a low score, I would be very happy."

Listen to this is human words?
and this……

"Except for the boring 100 points, in terms of my ability..."

"I'm so envious that you can only get so few points..."

Gradually, Yang Li finally understood what was going on.

She stood there like a repeater, recalling Chen Gong's words several times.

But I still don't understand what it means.

Isn’t 100 points bad?

This……

This is the first time Yang Li has seen such a person, a masochist!

Not happy about getting high marks in the test?
Also, are you envious of your own food?

Is there such a boast!
After a long time, Yang Li finally came back to her senses and suddenly the corner of her mouth twitched.

Good guy!
You bastard!

Are you here for the exam?

Aren't you here to cause trouble?

Yang Li was so angry that her teeth itched.

……

Obviously, Chen Gong couldn't hear.

After he arrived at the anatomy examination room, he read the paper carefully twice, and then realized that fortunately he had not done it...

Otherwise, you’ll lose again!

The morning exam ended successfully after Chen Gong successfully answered each question.

At noon, I drove to the biochemical experiment skills test center and had a quick lunch.

Then wait patiently.

There were obviously many people around who came to take the assessment.

Everyone is talking about the morning exam questions.

"This year's biochemistry is too difficult!"

"Who says it's not? I've never seen this type of question before. It seems simple, but it's actually full of traps!"

"Hey...it's so difficult. I feel like I might not be able to pass the written exam this year!"

"Before your results come out, don't talk about yourself like that. You have to think like this. What will happen if you pass the written test? It's the same as saying that you can pass the skill..."

"...You know how to comfort people!"

……

Listening to everyone's words, Chen Gong was truly envious.

Although Chen Gong didn't know why the type of test questions was so similar to the type of biochemistry simulation test he designed.

But...this is not a bad thing after all.

Because he knows very well that this type of question can improve people's biochemical thinking and improve their biochemical association ability.

If you really use this set of questions to assess, you can indeed screen out many good talents.

afternoon!

At around two o'clock, everyone began to enter the examination room, looking for the start of the exam.

First, before starting, draw lots to receive your own skill assessment items.

This level is to randomly select a skill for assessment.

Everyone's time is limited, and they are required to complete the experimental project within 30 minutes. ,

Chen Gong quickly got his envelope, but he couldn't open it.

After entering the examination room, hand the envelope directly to the examiner, and then start the operation after the examiner informs you of the project requirements.

At this time, Chen Gong also discovered that most of the people who came to participate in the experimenter assessment were relatively younger.

Because the junior laboratory technician qualification certificate has an age limit, you must be under 35 years old to participate.

The so-called junior experimenters actually include first-level and second-level experimenters.

Once you reach level three, you are an intermediate experimenter.

Levels [-], [-] and [-] are experimental specialists, which is very rare.

In this way, Chen Gong's age does not seem to be out of the ordinary.

quickly!

As the bell rang, the first batch of candidates began to enter the examination room.

Chen Gong stood up and walked towards his room.

After entering, the two examiners sat on chairs in the laboratory, and Chen respectfully submitted the envelope.

Liu Rong is from the Laboratory Department of the First Affiliated Hospital of Shanhe Medical University, and is also a staff member of the Biochemistry Research Institute of Dongshan Experimental Base.

Her partner today is Zhuang Baoshan, director of the Biochemistry Laboratory of Shanhe University of Traditional Chinese Medicine.

The two of them each had a form in their hands at this time.

The densely packed blank space above is the scoring area.

The test skills assessment actually focuses on details.

There are hidden pluses and minuses in many places.

In addition, there is also a certain proportion of comprehensive judgments on the candidates' ability to adapt to changes in circumstances, evaluation of experimental accidents, etc.

Because it is impossible for every experiment to be successful.

Accidents happen from time to time. At this time, mentality and adaptability will become very important.

Liu Rong could not see the condition of the candidates. After seeing Chen Gong, she nodded.

"Your assessment project today is: the separation of subcellular structures."

"Candidates, please be prepared."

"In 5 minutes, start your operation."

"Relevant utensils and test materials are already next to our separator."

After hearing this, Chen Gong was not surprised.

The separation of subcellular structures can only be said to be neither difficult nor easy.

after all……

This test requires the isolation of nuclei, mitochondria, microsomes, and cytoplasm.

For this test, what needs to be mastered is ultracentrifugation technology.

The so-called ultracentrifugation technology is more difficult than ordinary centrifugation techniques. It requires the application of powerful centrifugal force to separate, concentrate, and purify substances based on their sedimentation coefficient, mass, buoyancy factor, etc.

In recent years, ultracentrifugation technology has become more mature and has become an important technical method for separating, purifying, and identifying biological polymers.

Therefore, ultracentrifugation technology is no longer just an introductory technology in biochemistry, but has even become one of the main technologies in bioengineering.

This is fun!
Chen Gong took a deep breath and walked to the centrifuge nearby.

After seeing the instrument, Chen Gong's eyes suddenly widened and he was a little happy.

Good guy!
Swiss centrikonT-1055!

This is good stuff.

Chen Gong only dismantled this thing two or three times.

Although there are many such instruments in their school laboratory, they are very expensive and are closely watched by the experimenters on weekdays.

The laboratory that Yang Li often goes to is the German K-355 model, which is an outdated style.

All indicators of ultra-high speed are not as accurate as this!
In this regard, T-1055 does very well.

Chen Gong had used it several times, but... only dismantled it two or three times, so after seeing him, his hands instinctively felt itchy.

However, today is the assessment site, and Chen Gong knows that he cannot mess around...

So, after some adjustments, he decided to start his own operation.

According to conventional experimental techniques, Chen Gong needs to calculate the centrifugal force based on Newton's second law.

The centrifugal force (Fc) depends on the mass of the particle (m) and the centrifugal acceleration (a), that is, Fc= ma
Centrifugal acceleration depends on the angular velocity of rotation (ω)
and the radius of rotation (r), that is, a=ω2 r
Fc= m·ω2 r= m(2πn/60) 2·r
n is the number of revolutions per minute:

The magnitude of Fc is [g·cm/sec 2] (i.e. [dyne])
This is followed by calculating the relative centrifugal force (RCF).

RCF=Fc/Fg=m·ω2 r/m·g =ω2 r/g
g is the acceleration of gravity of the earth (take g=980cm/sec 2)
Finally, the settlement coefficient must be combined to obtain the final conclusion.

V=2(Dd)gr2/9η
V is the particle settling velocity, D is the density of the particle, d is the density of the solvent, eta is the viscosity of the solvent, g is the gravity constant, and r is the radius of the particle.

……

It can be used to separate organelles from tissue homogenates. The sedimentation sequence of the main cellular components is generally whole cells and cell fragments first, followed by nuclei, chloroplasts, mitochondria, lysosomes, microsomes and ribosomes.

……

However, Chen Gong has completely mastered and memorized all the data.

Therefore, it is enough to save a lot of time and start typing directly.

"Enter the centrifugation temperature!"

"Enter centrifugation time!"

"Enter centrifugal speed!"

……

Chen Gong chose density gradient centrifugation.

That is to say, different gradients are selected based on the different densities of cell nuclei, mitochondria, and particles.

This is relatively cumbersome.Time passes like this minute by minute.

The first time, 10 minutes later.

The tissue fluid homogenate was first separated from the cell nuclei, which are cells that have not been destroyed.

The subsequent supernatant needs to select a concentration and be separated again.

but……

Just as Chen Gong continued to start, he suddenly heard bursts of special sounds coming from the separator.

The sound wasn't harsh, but... it was obviously out of place.

To put it bluntly, this is the sound caused by abnormal working conditions.

The examiners Liu Rong and Zhuang Baoshan were both senior and they knew it right away...

This may be an instrument problem!

How to do?
Zhuang Baoshan was about to speak, but Liu Rong stopped him directly. He looked at the other person anxiously and signaled to relax.

Because emergencies are still one of the elements of the assessment.

And, most importantly, Chen Gong's previous performance was very good.

Half of the operation has been completed, and there are no problems with the subsequent methods and methods, including the details.

If there was no such emergency, it would obviously have to pass.

but……

Isn’t surprise a pattern in the laboratory?

As long as Chen Gong behaves calmly and calmly enough, whoever can deal with the situation in a timely manner, even if it is a power outage, or... choose other methods, as long as the current scene is satisfied, will be able to give a good score.

As for changing equipment?

I can only wait for the next candidate.

Because even if Chen Gong changed the equipment at this time, he would not be able to complete the experiment.

Because half the time has passed!

At this moment, Chen Gong was obviously stunned.

He turned around and glanced at the two examiners, but... saw no reaction from them.

Now, Chen Gong was a little curious!
Is this... also one of the assessment contents?

Chen Gong calmed down at this moment. He turned off the power without any hesitation.

After waiting for the speed to stop completely, he said to the examiner:

"One of the common obstacles of the T-1055 ultra-high-speed centrifuge is excessive noise."

"Special circumstances are currently occurring and the equipment needs to be inspected."

After speaking, Chen Gong directly turned on a switch on the back of the centrifuge, and a small device popped out.

Relying on a small wrench similar to the size of a finger, Chen Gong can completely dismantle the instrument.

but……

Chen Gong didn't dare waste time.

He still has an exam to continue.

Therefore, he must complete this experiment as soon as possible.

For a moment, he picked up the small wrench and quickly checked it.

“The centrifuge base is solid!”

"No wear on the centrifuge bearings!"

“There is no slack in the centrifuge conveyor belt…”

……

At this time, Liu Rong and Zhuang Baoshan were dumbfounded.

They have been examiners for so many years, and they have never encountered scenarios where candidates encountered machine failures.

It's just... I've seen people who were nervous, people who were panicked, and even people who were scared to cry.

but……

Is this the first time I’ve seen someone who not only showed no signs of nervousness, but even attempted to repair the machine?
For a moment, even Zhuang Baoshan couldn't control himself.

He glanced at Liu Rong, and then really wanted to speak...

But Liu Rong stopped him.

Although Liu Rong is a woman, she remains calm in times of crisis. Otherwise, could she become the female director of Dongshan Institute of Biochemistry?

Why don't you have two brushes? !

Liu Rong knew very well at this time that rash intervention would not change anything.

Chen Gong's behavior did not cause any damage to the instrument.

Machines are not that delicate.

Compared with the machine, Liu Rong wants to know what this child wants to do and what he can do!
The effect and value brought by a talent are far beyond what a machine can match.

The machine is broken and can be repaired!

Has the talent been buried?No one cares!

Liu Rong looked serious and didn't even take a step forward. He didn't say a word. He wanted to see... what this child was going to do!
She was willing to give Chen Gong enough freedom to make his own choices.

big deal...

So what if she takes on this responsibility?

At this time, Chen Gong was still undergoing maintenance, and his time was running out.

There are less than 8 minutes left until the end.

Chen Gong followed the disassembly of the parts.

No aging or wear of the bearings or loosening of internal parts was found.

As such...

Chen Gong's eyes suddenly lit up, yes...

It may be caused by the imbalance of the centrifugal rotor!
Chen Gong quickly opened the internal rotor structure of the centrifuge. Sure enough, Chen Gong clearly saw that there was an adherent sample on the rotor of the centrifuge!
That's why.

Generally speaking, the rotor of the centrifuge should be cleaned regularly, and the rotor should also be cleaned and coated with lubricating oil and sealing grease.

However, due to assessment, it was used yesterday, and during this period, long-term use was not processed in time.

This leads to the mixing of condensate water into the centrifuge cavity and rotor. At this time, once the sample leaks out, adhesion will form, causing the centrifuge rotor to be unbalanced.

Although it seems like only a subtle change.

but……

When an ultra-high-speed centrifuge is working, the rotor is the key. A slight load will cause imbalance and produce noise.

Chen Gong quickly took out the neutral washing machine, wiped it briefly, rinsed it with clean water, and then dried it before putting it back on.

And this time!
There is not much time left...

After Chen Gong finished pretending, he only had about 5 minutes left.

Chen Gong also quickly started the operation.

This time, he did not choose general density centrifugation.

Instead, differential centrifugation was chosen.

This is a method that uses different centrifugation speeds and centrifugation times to separate particles with different sedimentation speeds in batches at different centrifugation speeds and different centrifugation times.

Compared with the previous operation, the difficulty factor is higher, and the relative difficulty of control is also higher.

However, two things can be done in batches.

such as……

Low-speed centrifugation completes precipitation. After collection, ultra-high-speed centrifugation completes secondary precipitation.

Moreover, Chen Gong used the technique of differential centrifugation. After centrifugation, the supernatant can be separated from the sediment by pouring, and an angle rotor with a larger capacity can be used.

as predicted!

This time, Chen Gong directly chose to increase the rotor angle by 45 degrees!
As such.

Although the wall effect is serious and particle extrusion will occur, it can achieve better separation and precipitation in a short time.

……

Liu Rong sat aside.

After listening to the crazy rotation of the centrifuge, there was no movement at all, and my eyes suddenly became more exciting!
And his subsequent operations made Liu Rong clenched her hands.

She looked at Chen Gong, her eyes full of excitement and appreciation!

What a brave and resourceful boy.

I actually thought of using differential centrifugation, combined with the idea of ​​increasing the rotor angle.

This is really no ordinary person!

To be honest, Chen Gong's use of this ultra-high-speed rotor machine simply exceeded the level of ordinary experimenters.

Be bolder!

The road is wilder!

The operation is more detailed!

Think more actively!

Chen Gong is more agile than those experimenters.

Even an intermediate experimenter would not be able to imagine completing such an operation within a few minutes.

They will rigorously choose to give up.

but……

This kid didn't do that.

Instead, it gives full play to the various functions of the machine.

Liu Rong was really surprised!
It's hard to believe that such an operation happened during the assessment of a junior laboratory technician, and it was also an assessment of...a first-level entry-level laboratory technician.

This is somewhat unbelievable to say the least.

……

quickly!

Chen Gong finally completed the final operation before the time came.

Chen Gong wiped the sweat from his forehead, turned around, and bowed to the two teachers.

"Thank you teachers!"

"Feel sorry!"

"I'm giving you trouble."

"But... I suggest to strengthen the maintenance of this T1055. Although its conveyor belt is not loose, but... I estimate that it has only been used seven or eight times, and there will definitely be problems."

After Chen Gong finished speaking, he stood up and left.

Zhuang Baoshan said to Liu Rong with a shocked expression:

"Unbelievable!"

"This kid...can actually repair a centrifuge?"

"This is an ultra-high-speed centrifuge...how brave!"

“Not only was there no tension at all, he even had the machine repaired!”

"It's really terrifying!"

Liu Rong nodded, smiled slightly, and looked at Zhuang Baoshan:
"Director Zhuang, don't be anxious if something happens in the future!"

"Look, young man, if you don't force him, he doesn't know how good he is!"

"You also saw the subsequent operations."

"Differential separation!"

"Zone effect!"

"Rotor adjustment."

"Twice the difference in effectiveness!"

"..."

"Do you think a person who has taken the junior entry examination can have such strength?"

"Unbelievable!"

"I don't know what teacher can train such students!"

"It really surprised me."

Liu Rong's eyes were a little more envious.

"If this child is in my hands, I can make him the youngest experimental specialist in the country in less than five years!"

"This is simply a good material born for experimentation!"

Liu Rong's praise was effusive and unstinting.

Zhuang Baoshan looked a little dumbfounded.

"I haven't seen this kid before. He doesn't look big. He should be a graduate student, right?"

Liu Rong shook her head: "No!"

"Definitely not a graduate student!"

“He doesn’t have the stereotype of a graduate student.”

"Definitely a college student!"

"Shanhe University..."

"I don't know if I've done an internship yet..."

Liu Rong shook her head. They couldn't see the candidates' information, so they had no choice but to give up.

"Okay, let's rate it!"

"Yeah, score..."

"how to spell?"

Liu Rong smiled slightly: "Other than full marks, I can't think of a second answer!"

After a while, Zhuang Baoshan looked at Liu Rong: "By the way, how did you write the evaluation?"

Liu Rong smiled: "You are gifted, have a tiger in your heart, and have a promising future!"

"What about you?"

Zhuang Baoshan wanted to hold the paper at this moment. It was so embarrassing. Look at what others had written. They were so literate. Look at what he had written...

"Instead of being nervous, the candidates tried to fix the machine..."

……

……

ps: First order: around 3000.

Fairly passable.

As for the results, starting from this book, it is still acceptable. After all, the threshold is relatively high, and the start is not in the hospital, and it does not use things that veterans are good at.

However, people always need to change. It is necessary to try to get out of the simple comfort zone and change the methods.

Taking my time, I feel confident.

However, for a veteran who writes books, hahahaha...it's not as good as the first book.

Hey……

If I say too much, I will cry.

There are many reasons, so I won’t make a summary, because thinking too much about this kind of thing will affect my enthusiasm. I’d better write this book well.

Still, please support!

Big update, definitely a big update, 16000 will be delivered, everyone will be satisfied.

(End of this chapter)