Those years when I hung up and upgraded in the hospital

Chapter 233 Participate in the operation

"Wang Huan, Dr. Liu Xianming and I have agreed to come here today, take the laboratory exam in advance, and then pay the fee." Fang Yun said with a smile.

Wang Huan's mind suddenly shut down.

The safety examination and guarantee fees in the laboratory are the procedures and steps that foreign experimenters need to take when entering the laboratory. Fang Yun is a training doctor, why does he do all this?

However, since Fang Yun is already here, it must have been agreed by the big bosses, but it is not something he can ask.

Then he brought Fang Yun in.

At this time, several people who were sitting and editing experimental data turned their heads and looked over. Seeing that Fang Yun was unfamiliar, they asked.

"Xiao Wang, who is this?" one of them asked cautiously.

"Senior Brother Liao, this is Fang Yun, the advanced training brother in our group, here to see Senior Brother Xianming." Wang Huan explained helpfully.

"Liao Lele, Brother Le." Wang Huan introduced Liao Lele's identity to Fang Yun.

Liao Lele is an academic doctoral candidate of Professor An Luming. Apart from Liu Xianming, he is currently the largest scientific research manager in Professor An Luming's group. Even to a certain extent, Liu Xianming has to accept Liao Lele's supervision in the laboratory.

After all, although Liu Xianming is a third-year Ph.D. senior, he certainly doesn't spend as much time in the laboratory as Liao Lele.

Liao Lele knew what happened to Fang Yun, so she stood up politely and stretched out her hand: "Brother Yun, in this case, you go to the demonstration classroom over there first."

"I'll give you two sets of questions and you can do them. This is a procedure."

To enter the laboratory, let alone anything else, you must be familiar with the laboratory's safety management regulations, especially the emergency handling procedures exam.

Fang Yun could also understand that it was customary to follow the local customs when entering the countryside, let alone entering a laboratory.

The exam questions are not difficult and the process is not complicated. There are two sets of questions, mainly related to safety issues in the laboratory.

Fang Yun finished it quickly. The final test results are all formatted. This is not to make things difficult, but to ensure that you at least know what is going on.

Fang Yun's previous signature procedures were completed by someone in the clinic. The person in charge of the laboratory was the professor and associate professor of An Luming's group. There were no other persons in charge, so it was okay to sign.

However, Fang Yun was still taken to the safety management office of the laboratory building by Liao Lele, and paid a deposit of up to two thousand yuan, just in case Fang Yun encountered something and caused any losses, the money would be used to deduct.

Although he has to lose money, Fang Yun still has to pay.

The laboratory here is much more formal than the place Dr. Dong Yue from Xiangzhou People's Hospital took him to when he was in Ji City.

After everything was ready, when Fang Yun returned to the laboratory, Liu Xianming came out.

"Brother Yun, I have been doing experiments before, but then my junior brothers told me that you were here. You should have said hello in advance." Liu Xianming was very polite.

He and Fang Yun each had their own needs. He envied Fang Yun's current basic skills, and Fang Yun envied his accumulation of scientific research.

"I'm here in advance. I originally planned to run the program!" Fang Yun was a little embarrassed.

I was a little impatient.

Liu Xianming understood: "It doesn't matter. Brother Yun, you came just in time. I plan to resuscitate a batch of cells today, which are for osteosarcoma. Brother Yun, look, if it's convenient for you, let's resuscitate them together."

"Just a seed for you."

The seeds Liu Xianming refers to are not website seeds as mentioned on the Internet, but real tumor seeds. They are purchased cell lines. The cell raw materials obtained after passage in previous generations and cryopreserved are very precious experimental consumables.

"Thank you Xianming." Fang Yun sighed secretly in his heart. This is indeed a laboratory of a big hospital, it is so generous.

You know, when Dong Yue was doing experiments before, such cell seeds were all brought by him during his PhD. If you want to buy them separately, a new osteosarcoma will cost an estimated five to six thousand, or even tens of thousands.

Of course, generally speaking, there will be multiple sets of cell lines in a laboratory, from various sources, and you can use them directly after entering the laboratory.

When publishing articles, we look at the supporting units and laboratories, not your personal name.

If you don't have a supporting institution or laboratory, then you probably have to show a purchase list, especially a person like Fang Yun who has no scientific research foundation and no teacher endorsement.

"Brother Yun, you're welcome. It's all a trivial matter."

"Master has already said that I will give you, Brother Yun, tens of thousands of dollars to conduct experiments alone. If you can make Master say this, we will all need more support from you, Brother Yun." Liu Xianming joked.

But the meaning behind the words is not rough.

Fang Yun is not An Luming's student. Logically speaking, An Luming can not give Fang Yun any start-up funds and funds for scientific research. You can find a solution by yourself.

However, An Luming did not do this. Although the money given to Fang Yun was not very large, less than 1% of the project fund in the group, this behavior was a hint.

If Professor An Luming doesn't take Fang Yun seriously, why don't he give you shitty money to do experiments?

Fang Yun had already expressed his gratitude in front of An Luming, so there was no need to repeat it now.

The two of them walked into the cell room.

Liu Xianming did not make any preliminary preparations. The experiment was Fang Yun's. The reagents, such as culture medium, buffer, trypsin and other operating materials, were all produced in the laboratory. If Fang Yun did not perform the operations himself, then subsequent experiments would be impossible. There is no need to talk anymore.

Even Liu Xianming had some intentions of taking the exam for Fang Yun.

Of course, cell recovery is not difficult in itself. Fang Yun went to Liu Xianming's refrigerator, took the culture medium and other reagents, and placed them in a 37°C water bath.

The recovery of tumor cells needs to match the temperature in the body. Cold culture media cannot be added directly. Although tumor cells may survive, a number of them will die, so all conditions must be strict.

As for pipettes, centrifuge tubes, etc., they are placed inside the cell operating table for blue light disinfection.

"Dr. Liu, if you still have your own experiments, you can ask others to come over to help me guide and review the process. There is no need to follow me throughout the process." Fang Yun said.

Liu Xianming was doing the experiment himself. He was inserted in the middle. He had no obligation to teach. If he wanted to see whether his operation complied with the process, there was no need to be the examiner himself.

"Brother Yun, it's okay today. I'm waiting for you to go to the practice room with me, Brother Yun."

"Oh, by the way, Brother Yun, Lin Xin, who is following you, is a graduate student at our school and has been in contact with the teacher recently."

"The teacher agreed, so I hope that Brother Yun, when you teach in the future, you can go more in-depth and train him as a graduate student in trauma surgery, such as some basic skills, film reading, report reading, medical record writing and surgery Basic operations can be developed to a deeper level." Liu Xianming hinted to Fang Yun.

"Lin Xin wants to study for a graduate degree in orthopedics? That's a good thing." Fang Yun was also very happy when he heard this.

"This Lin Xin's talent is pretty good. He hasn't even graduated from his bachelor's degree yet. If he takes his time in the future, his achievements may be much higher than mine." Liu Xianming was afraid that Fang Yun would not understand the value of Lin Xin at his current age, so he explained quickly.

(End of this chapter)