Galactic Tech Empire

Chapter 479 Gene Medicine ([-])

When the Martian Territory was in a hurry by the virus incident, the new Keelung city base on the moon.

Huang Haojie is working with Michelle, Zhao Zhangde, and Takahashi Kenji to develop the Sun Ladder Virus. At this time, their research and development work has advanced by leaps and bounds.

"I didn't expect that allicin could actually inhibit the sun ladder, and the effect is so great that it is a natural inhibitor." Zhao Zhangde said standing outside the testing area.

Beside him are Huang Haojie, Michelle and others.

In the test area, I don’t know how many mice were injected with the improved version of the sun ladder. It was in pain, howling, and struggling, but the humans outside the shield turned a blind eye to it. Obviously, humans are more concerned about the success of the experiment.

After a period of intense struggle, the little white mouse has turned into a big muscular mouse under the supply of nutrient solution, but although its strength has increased a lot, it still can't break free from the titanium alloy lock. After the big mouse found that it couldn't break free, Then he calmed down and stared at the humans outside.

"Success!" Michelle grabbed her hand excitedly.

"It's still too early to say success, let's check the experimental body." Huang Haojie touched his chin and said.

Kenji Takahashi hurriedly operated the experimental platform, and conducted a full-body inspection on the successfully strengthened rats, and after a while countless test data popped up on the holographic computer, Huang Haojie looked at these data and shook his head: "Success is half done. "

Michelle and Zhao Zhangde also looked at the data quickly. After a while, they also understood why Huang Haojie said that he was half successful, because the genetic mutation of the mouse was too strong, and there was an obvious phenomenon of atavism. The current gene series There is a 64% difference from before, which is equivalent to changing a body.

"It seems that the dose is too high. We need to weaken the dose and mutation rate of the Sun Ladder." Zhao Zhangde recorded some data and revised directions while talking.

Looking at the experimental data, Huang Haojie knew that the use of the sun ladder would inevitably induce gene mutations. However, if too large a dose of the sun ladder is used at one time, it will not only cause high mutations in the genes of the organism, but also cause a series of problems. sequelae.

For example, the temperament has changed drastically, becoming moody and aggressive, or atavism, or mutation and alienation, and may have an extra hand or a foot.

In the virus era, the probability of this happening was about 30%. Such mutations were acceptable in that era, but in the 21st century, I believe that most people are unwilling to become heterogeneous, especially those with obvious appearance characteristics.

"Michelle, Zhangde, I, and I will continue to modify the sun ladder, and you, Takahashi, will continue to test the remaining sun ladder improvement reagents." Huang Haojie ordered.

"Okay, head of state." Takahashi Kenji quickly bowed and replied.

Inside the virus lab.

Huang Haojie, Michelle, and Zhao Zhangde are all inside, but according to the safety regulations of the biochemical virus laboratory, the three of them will not really come into contact with the biochemical virus, but will operate the virus through a substitute robot or an auxiliary robot.

The experimental accidents in many science fiction movies are often not because the virus is too powerful, but because the experimenters are too deadly, such as direct close contact with biochemical viruses or experimental subjects, or insufficient safety measures, or the experimenters do not follow the routine.

And the alliance's laboratories, especially the biochemical laboratories, are strictly guarded against death, so that all experimental accidents can be prevented.

For example, in the No. [-] camp where they are now, three layers of condensed matter shields are set up. Once the virus leaks, under the isolation of layers, the possibility of the virus being leaked is zero, unless it is intentionally spread by humans, but the alliance can deal with these. People who come into contact with the virus are subject to very strict scrutiny, and the laboratory is monitored by artificial intelligence around the clock, which basically eliminates the possibility of human intention.

“米歇尔，将太阳阶梯RNA第一链的外显子37—8—13的异常表达35号序列修改一下成为异常表达37号甲序列。”黄豪杰看着太阳阶梯的全息基因螺旋体吩咐道。

"OK."

Michelle's fingers fluttered over the holographic projection, quickly modifying the exon sequence of the solar ladder.

And Zhao Zhangde is modifying some non-structural genes of the solar ladder (a non-coding DNA segment on both sides of the structural gene, also known as the flanking sequence, which is involved in the regulation of gene expression). He is adjusting and modifying while checking the simulation results of the supercomputer.

There are more than a dozen researchers in the laboratory who are also modifying the solar ladder virus.

"Group Zhao, the promoter of the non-structural gene 9-6-15 sequence has collapsed, and your modification conflicts."

Zhao Zhangde looked at the situation of the 9-6-15 series of non-structural genes, and re-simulated it again with supercomputing. The result of the supercomputing simulation was [the promoter RNA polymerase specifically recognizes the abnormal DNA sequence that binds and initiates transcription, and the transcription initiation Site and response element sequences generate conflicting responses. ]
"Xiao Zhang, you can modify the sequence of the non-structural gene 9-6-15 to a more stable sequence." Zhao Zhangde ordered.

"Ok."

Zhao Zhangde also carefully studied the upstream promoter elements in the non-structural genes of the sun ladder, that is, some specific DNA sequences upstream of the TATA box. Trans-acting factors can bind to these elements to regulate the transcription efficiency of genes.

RNA sequences that affect the transcription of sun ladder genes through trans-acting factors and binding to specific cis-acting elements.

After reading this part, he found two minor problems and then revised it. Then he asked Huang Haojie: "Führer, have you modified the exon 33-3-76 sequence? Is it still the previous version? "

"It's still the previous version, and this part has not been modified for the time being." Huang Haojie turned his head and replied.

"Okay." Zhao Zhangde hurriedly continued to look at the situation of exons and introns. He looked at the holographic gene spirochete and modified one or two gene series from time to time. With the genetic programming technology of the Luoxing people, they really dare not modify the genetic sequence of the Sun Staircase randomly.

He glanced at the supercalculated Poly(A) tailing signal results. The conserved AAUAAA sequence at the end of the structural gene and the downstream GT or T-rich region are recognized by polyadenylation-specific factors, and 3 is added to the 176′ end of the mRNA. A.

Then a non-directional enhancer was combined with a trans-acting factor to enhance the transcriptional activity of the sun ladder, but Zhao Zhangde modified the sequence of the enhancer, because the general enhancer is effective at any position in the gene, but he Targeted enhancers are now required.

A directional silencer was added behind the enhancer, which is used for gene expression negative regulatory elements, combined with trans-acting factors, and directed to inhibit the transcriptional activity of a specific gene in the solar ladder.

After a busy half a day, Zhao Zhangde began to adjust the gene series of the response elements of the sun ladder. The response elements can bind to the activated information molecule receptors and regulate the specific DNA sequence of gene expression.

This part is related to the strengthening of the combination of the solar ladder and the human gene, and there are many places that need attention and adjustment.

Speaking of which, the Anluo people and humans are both carbon-based organisms, which greatly reduces the difficulty for Zhao Zhangde and others to transform the solar ladder. After all, they are also carbon-based organisms, and many things are similar in nature, but the Anluo people The study of genes is more in-depth and comprehensive than that of humans.

(End of this chapter)