Healing Hand of Rebirth

Chapter 76 Let's talk about business (3)
But thinking about it carefully, besides not dealing with He Liqin's mother and daughter, he also had a grudge against him.Old Wu handled things very comprehensively. In addition to the things he wanted to investigate, he also attached Zhang Wenkang's current contact information.

Originally, if Zhong Luo hadn't called out those fake medicines by name, Zhang Wenkang would have done pretty well.His uncle is the director of the Food and Drug Administration of City B, with power, and he is rich, and the two of them have cooperated seamlessly for so many years.

After going to the hospital the next day, Zhong Luo couldn't wait to go to the office with those materials and prepared to tell Mr. Bian the good news.It's just that she clearly remembered that the things were placed on the table, so why did they disappear?
"Doctor Zhong, what are you looking for?" Shang Xun saw Zhong Luo anxiously looking for something outside the window, with a smile on his lips, and then entered the office, asking with concern.

Seeing that it was Shang Xun, Zhong Luo suddenly remembered that she was the last one to leave the office last night, so she asked, "By the way, Xiao Shang, when you left the office last night, did you see the pictures on my desk? Paper."

Shang Xun shook his head blankly: "No, I closed the window and left after you left."

Zhong Luo began to search everywhere by herself again, but she searched everywhere, but there was no trace.

"Is it something important?"

"Well, it's about the people."

Shang Xun's heart skipped a beat!It's about human life!
She felt guilty for a while, although she really wanted Zhong Luo to leave the hospital and stop hanging around Yang Jun all the time, but she didn't have the guts to take someone's life.

She asked palely: "Those things are really so important?"

Zhong Luo replied: "It's very important, Bian Lao's granddaughter Bian Susu is still waiting for these things to save her life."

Shang Xun was very flustered and didn't want to stay here any longer. She said to Zhong Luo: "There are patients waiting for me down there, so I'll go to Dr. Zhong first."

After speaking, he ran away without looking back.

Zhong Luo, who was concentrating on finding something, didn't notice the unusual boredom in her eyes.

After Shang Xun left, Zhong Luo searched for a while, but still found nothing, so she couldn't help but sat down on the chair.After Susu gave the patient some drips, when she passed by Zhong Luo's office, she saw Zhong Luo sitting on a chair with a slumped expression, so she went over and asked, "What's the matter, Dr. Zhong?"

"Something is missing."

"Is it something important?"

Zhong Luo nodded heavily: "It's a life-saving thing."

Susu nodded clearly, "That's really important." After finishing speaking, she left the office with her things, and suddenly looked back at Zhong Luo, and said, "I just saw that Doctor Yang seemed to be looking for you. .”

"Well, I see."

She then got up and followed Susu out of the office.Just when I turned my head, I saw that a window in the office was still open, and then I looked at my desk. Could it be that those few pieces of paper were blown away by the wind?
She came to the window and looked. If it was blown away by the wind, it would be really hard to find the few pieces of paper.Disappointed, he turned and left the office.

Seeing Yang Jun sitting in the biochemical laboratory, Zhong Luo also came in and asked, "I heard you were looking for me."

"Yeah, none of the compounds that we screened out yesterday are naturally occurring. I have thought about several ways of chemical synthesis, but none of them work. I also consulted with some of my chemical brothers, and finally got The conclusion is that these things cannot be done chemically."

Zhong Luo's eyes lit up: "Do you still remember the structures of those compounds?"

Yang Jun looked at her in puzzlement and surprise: "Don't you save it in the USB flash drive?"

Zhong Luo was embarrassed, but she did not save it.All the worries of the morning were gone, and Zhong Luo's mood improved a lot. Thinking of what Yang Jun said just now, she explained: "These are all biologically active substances. They must have certain biological activity. Their structural units are amino acids. Although there are several chemical groups that are not commonly seen, they cannot be synthesized by chemical methods."

Yang Jun frowned and asked, "Then what should we do?"

Zhong Luo smiled and replied, "I have no other choice but to try genetic engineering."

Genetic engineering is also known as gene splicing technology and DNA recombination technology. There are four bases on the DNA chain, which are A, T, C, and G. There are also four bases corresponding to the transcription of DNA into RNA, corresponding to A, U, C, and G. Every three bases Base pairing represents an amino acid, for example, AUG represents methionine, and GUG represents valine, etc., which are codons.

A protein is made up of amino acids connected one by one, so as long as its amino acid composition can be measured from that compound, and its codons can be reversely deduced, so as to deduce the sequence of bases on its RNA, you can know The base sequence of that stretch of DNA.Strictly speaking, this is protein engineering.

Theoretically speaking, all this is not complicated. The complicated thing is how to find that piece of DNA. Moreover, for this kind of 'artificial' protein, there may be no such piece of gene in any organism, and it needs to be digested with restriction endonuclease. Cut off, take out the segment you want, and then connect it with a thermostable polymerase.

The difficulty is that these two tool enzymes are hard to find.

Especially endonuclease, it must cut out the gene at the site it wants.

Once the gene is produced, its expression must be carried out in the living body, so cell engineering is still required.That is, the gene is integrated into the DNA of the microorganism, so that it can be expressed in the microorganism to produce the target protein.

After the plan is determined, it is continuous experimentation.

Although all this seems not complicated, it is a heavy workload and requires high skills in the operation of the experiment. Zhong Luo said, "It's really not enough just the two of us."

"We don't need the two of us, don't we still have graduate students of genetic and molecular biology who come here to do experiments? Leave it to them." Yang Jun remembered that his father seemed to have a few doctoral students in his hand, and he could also send Came over as a medical guide, so I mentioned it again.

Zhong Luo asked in surprise: "Really? Is it possible?"

Yang Jun pursed his lips and smiled, with an imperceptible pampering: "I won't lie to you if I lie to anyone."

"Well, this experiment needs to be done as soon as possible, preferably within one and a half months."

"Let me tell you, if we don't have any patients, we can come to supervise and follow up."

Zhong Luo agreed: "This is a must."

(End of this chapter)