Brainoid

On Tuesday, Qi Min finally went to the laboratory on time. Although she is not actually that dedicated, it would not be good if Zhang Zhiyong's people arrived and found that she was not there.

Since Qi Min arrived at the laboratory, he followed Ke Yue to prepare the rats. The fluorescent label is to be labeled on the nerve cells connected to the electrodes near the corpus callosum of the rat, so the rat still needs to be operated on. Rats who undergo surgeries so frequently are actually very susceptible to infection, so the rats need to be carefully disinfected near the wounds.

However, this time Qi Min was trying peptide quantum dots for fluorescent labeling. It was still an experimental study, so he did not use rats that were already connected to the corpus callosum interface, but used new rats. She and Ke Yue first made an incision on the rat's head that simulated the connection to the corpus callosum, and then used this incision to fluorescently label nerve cells.

Just as Qi Min was wiping the skin of the rat's head with alcohol cotton, suddenly the cell phone in his pocket rang.

Most of them are sending peptide quantum dots. Qi Min said hello to Ke Li, then walked out of the clean room and took off his gloves. He took out his mobile phone and saw that it was Zhang Zhiyong: "Hey, how are you?"

"I'm ready. It's downstairs in your department." Zhang Zhiyong said, "Where is your laboratory?"

Qi Min didn't expect it to be delivered by him in person, so he said with some embarrassment: "I'm in the clean room on the third floor now. How about... you come up directly? There are sterilized lab coats here..."

"Are you being a rat now? It looks like I came just in time." Zhang Zhiyong smiled.

Qi Min hung up the phone, changed his headgear, gloves, mask, and shoe covers, sprayed 75% ethanol for disinfection, and then passed through the air shower room before re-entering the clean room.

Ke Yue asked while opening the rat's skull: "Have the peptide quantum dots arrived?"

"We'll be there soon." Qi Min said with some shame, "How about... I'd better use your rat to operate it, I haven't finished it yet."

Ke Li knew that she was slow, and this was already a routine operation for them to cooperate in experiments. It's just pity that the rat assigned to Qi Min's operation had to lie in the safety cabinet for such a long time after being anesthetized.

Qi Min watched Ke Li quickly perform a fake operation on the rat, and then he heard the sound of the door opening of the preparation room outside the clean room a short while later. After a rustling sound of putting on shoes and gloves, the air shower room opened, another blow of air came, and then "fully armed" Zhang Zhiyong entered the clean room carrying an ice box.

As soon as he came in, he looked around and said jokingly: "Oh, the biology department's laboratory turns out to be so clean."

"This is a clean room for public use for animal experiments. The laboratory is not so tidy." Qi Min explained with a smile.

"It's really troublesome to get in here." Zhang Zhiyong placed the ice box on the central experimental table and took out a 15mL centrifuge tube wrapped in aluminum foil. "The peptide quantum dot solution should be stored at 4°C in the dark. You guys Where is the refrigerator?”

"Our laboratory is downstairs... why don't we put it in the ice box first." Qi Min said with some embarrassment.

"Okay." Zhang Zhiyong took out another 1.5mL small centrifuge tube, "Can you, the rat, do it?"

Qi Min glanced at Ke Yue, who was a little puzzled by this person who started shouting as soon as he came in. He turned to Zhang Zhiyong and said, "The wound has been prepared. Does it need to be cleaned?"

Zhang Zhiyong looked at Ke Yue, who was sitting in front of the safety cabinet, and said to Qi Min: "I don't know how your biology works. But should I teach him, or should I teach you directly?"

Qi Min forgot to introduce Ke Yue to Zhang Zhiyong at first, but he knew with his toes that the people doing experiments with Qi Min were either from her lab or from the labs of her collaborators. In short, they were all her subordinates. .

Zhang Zhiyong, like most PIs with some qualifications in the school, has a superior-to-subordinate attitude toward his doctoral students and technicians. But Qi Min was usually much kinder to Ke Li and the others, and he didn't want Ke Li to be ordered by Zhang Zhiyong, so he said, "You'd better teach me directly."

Ke Yue stood up from the chair inexplicably, and Qi Min sat in his place. He disinfected his gloves with 75% alcohol and reached into the safety cabinet.

And since she was conducting the experiment herself, Zhang Zhiyong was too embarrassed to stand aside and give instructions, so he also found a chair to sit on.

"You should spray some alcohol." Qi Min joked to him, "Actually, if you don't pass the experimental safety test of the biology department, you can't enter the clean room."

"The rules are dead, but people are alive. Can you, the head of the biology department, still not let me in here?" Zhang Zhiyong also jokingly added, and then said, "You take this mouse's... brain, Rinse it. It's kind of disgusting to look at it like that."

In fact, the part exposed in the safety cabinet is not the cerebral cortex tissue of the rat, but only the skull. The corpus callosum interface is minimally invasive and requires a needle to be inserted. However, Qi Min still rinsed the wound with saline.

"It doesn't feel right...the brain shouldn't be so smooth," Zhang Zhiyong said again.

"This is the skull, and the fluorescent substance must be injected through the pinhole." Qi Min explained.

"Then why are you flushing it?" Zhang Zhiyong said immediately, "Oh my God, you flushed the surface with water. Now there is water in the pinhole. Won't the peptide quantum dots be diluted?"

Qi Min was helpless: "You asked me to rush."

"Who is the biological engineer..." Zhang Zhiyong was also helpless, "How come you are still unreliable even though you are such an adult - do you need to think more and understand more?"

He said "think more" twice in a row. Qi Min vaguely felt that there was a pitfall in it, but still agreed: "Yes, yes. But now it has been diluted, and there is nothing we can do about it."

"Why is there no way?" Zhang Zhiyong said again, "The peptide quantum dots must be diluted with a suitable solution before use. Just lower the dilution factor. So you have to think more, and doing experiments is a combination of brain power and physical strength. , I can only tell you that you have to think more beforehand."

Doesn't this sound too inconsistent? Qi Min finally came to his senses. "Think more" and "I can only tell you that you have to think more before" were what Zhang Beihai's father said to him in "The Three-Body Problem".

"Wait, are you taking advantage of me?!" Qi Min turned to look at Zhang Zhiyong.

Who knew that Zhang Zhiyong said: "You just realized it? His reaction was too slow. Look at him -" He turned back and glanced at Ke Li, "He started laughing a long time ago."

It was really boring. Qi Min felt that Zhang Zhiyong's brain was not normal, maybe he had read too many science fiction novels. However, she herself felt that it was not inappropriate to say "think more" at the beginning. This meme is actually quite appropriate to use here.

Of course, the "appropriate" here refers to the appropriate use when she takes advantage of others.

But even if you are taken advantage of by using this kind of niche meme, you still have to do the experiment. Zhang Zhiyong carried out gradient dilution of the peptide quantum dots to prevent the quantum dots, which are nanoparticle colloids, from agglomerating. Then Qi Min carefully injected the diluted peptide quantum dots into the pinhole of the rat simulation interface.

After five minutes, Zhang Zhiyong asked Qi Min to rinse the pinhole with physiological saline to flush out the unbound peptide quantum dots. This is considered to be the completion of fluorescent labeling.

Taking the anesthetized rat out of the safety cabinet, Zhang Zhiyong instructed: "If you want to maintain it for a period of time, you have to avoid light. Also, remember to take immunosuppressants and the like. I remember that peptides have antigenicity. of."

These are all clichés for people who work as animals, Qi Min just agreed.

Then Zhang Zhiyong glanced at the watch in the clean room and said to her: "It's already past eleven o'clock now, shall we go have dinner?"

Qi Min and Ke Li couldn't help but look at each other. They used to do experiments together and go to dinner together, but Zhang Zhiyong had no intention of inviting Ke Li at all.

I don’t know what Ke Li was thinking, but Qi Min knew that Zhang Zhiyong’s attitude toward doctoral students was not as kind as hers, so he simply agreed: “Okay, let’s go.”

Playing the plot of Shining Love, I discovered that Qin Yi is a liar. I was so angry that I wanted to throw away his designer shadow and suit. Yes, it’s so zqsg, anyone who dares to lie to my girlfriend (?) will die! ! !

(This version of Nuan Nuan is so over the top. I never expected that my girlfriend is a woman who eats potato chips, watches TV, lies in bed, plays with her mobile phone, and does a plank for less than five seconds all day long (wrong)... I feel that I am already A waste of a house falling in love with a paper man...)