Doctor’s Life Simulator

At the same time, on the far side of the ocean, an editor of the "cell" magazine in the United States received a paper submission from China. After reading it, he thought it was very interesting. If the author proposed the ICR mouse inbred line , if it is as good as he introduced, it can completely replace the guinea pigs in the current experimental animal world and become the gold standard in the industry.

This is an epoch-making event. The editor smelled the unusualness of it, immediately printed out the paper, and walked into the office of the editor-in-chief Alex.

Alex, who is in his 60s this year, has dazzling blond hair and a face that is as tough as a knife and axe. The whole person gives a sense of authority, and he is reviewing some papers very seriously.

Alex is very well-known and influential internationally, and his own resume is also shining. He just retired from the position of technical consultant of Johnson & Johnson and joined "cell" magazine. He is a very prestigious person. , is also a powerful editor-in-chief, and is also the director of the research institute under "cell".

Seeing the editor knocking on the door, Alex looked up and seemed a little dissatisfied: "I didn't say, I'm picking the cover article of the next issue these two days, don't come in to disturb me if there is nothing important."

The editor handed over the printout of the paper in his hand: "Editor-in-chief, this paper is very interesting, I think you will be interested."

The editor knows Alex's temper very well. He is full of passion and keenness for this kind of groundbreaking paper and research.

Alex was a little surprised, took the paper from the editor's hand, glanced at the author, it turned out to be a doctor from China, was a little surprised, then opened the content and read it carefully.

After reading it again, Alex put down the paper, pondered for a while, picked it up and read it again.

"The inbred line of ICR mice has more traits than all the inbred lines bred in the world. If this is really achieved, I am afraid it will cause a sensation in the animal experimentation community."

With Alex's insight, he certainly understands the influence of this paper, which is absolutely epoch-making.

More than two-thirds of animal experiments use mice, so an inbred line of mice with better traits and more stability is enough to drive all pharmaceutical companies and scientific research units crazy.

In addition to its editorial department, the journal "cell" also has a subordinate research institute with part-time experts and researchers in various disciplines, which is a top scientific research institution in multidisciplinary fields.

The source of funds for the research institute is Pfizer, Johnson & Johnson, GlaxoSmithKline and other pharmaceutical giants, which are highly authoritative in the world.

After reading Qin Lang's paper, Alex had a crazy idea, that is, to conduct a verification test through the cell's subordinate research institute, as long as the excellent traits of the inbred line of ICR mice are confirmed, then the animal will be created immediately. A new gold standard for experimental mouse inbred strains in the experimental field, an authoritative new international standard.

In the future, all mice produced by animal companies will be based on ICR mice.

Alex thought for a while and said directly: "You print ten copies of this paper, and at the same time notify all the heads of the institute to be present. I will hold a large-scale academic exchange meeting of the institute."

...

...

At this time, Qin Lang has also sorted out the first draft of his topic.

When he came to Professor Shenxi's office and saw Qin Lang, Shenxi was also curious about what new ideas his student had come up with these days? Have you given up on the idea of ​​going into the pharmaceutical field.

"Qin Lang, how are you preparing for your project?"

Qin Lang directly handed his first draft to Shen Xi and said, "Teacher, I have changed my mind. I am going to put aside the research and development of new anti-tumor drugs. This time, the topic I proposed is: "In the laboratory, for improvement Evaluation of the traits, stability and superiority of ICR mouse inbred strains compared with traditional Kunming white mice""

"ICR mice?"

Shen Xi was a little dazed. She was naturally very familiar with the field of animal experimentation, but she had never heard of an inbred line of ICR mice.

"The so-called modified ICR mouse is a brand new inbred line that my subject intends to study this time."

Qin Lang said very directly.

"Are you going to improve the experimental mouse, and then evaluate and study the traits, stability, and superiority of this mouse?"

Shen Xi was once again startled by Qin Lang, why is this kid so wild?

Also, I don’t know that your graduate tutor, Shen Xi, and I, mainly researches in the field of tumor prevention and treatment, and is of first-class level in the whole country. I want to play with experimental mice without hugging such an excellent tutor’s thighs?

"Teacher, in fact, I already have a preliminary theoretical research foundation, and now I need to induce the first batch of ICR mice in the laboratory. I have established a cooperation agreement with our Zhejiang Mingshi Pharmaceutical Group, and the other party has provided me with five hundred In a flat rat house, as long as the first batch of mice can leave the laboratory, I can cultivate a large number of ICR mice in the experimental base of Mingshi Pharmaceutical Group. My project this time is to want the teachers and students of the whole school to cooperate with me. These inbred lines of ICR mice were scored and evaluated."

"I am willing to provide 100,000 improved ICR mice to the college for free, and I will also pay for the entire project at my own expense. As long as there is one request, it is to provide my laboratory for the induction of the first batch of mice."

Qin Lang directly showed his trump card. Rich people are so simple and unpretentious. Taking advantage of the talent, Qin Lang plans to mobilize the teachers and students of the entire Zijingang First Academy to do research for him.

To be honest, for the teachers and students of the college, the general project funding is only 20,000 to 30,000 yuan, and the experimental mice often take up 5,000 or 6,000 yuan. If someone can provide mice for free, they are of course very willing.

In itself, this kind of evaluation experiment is very simple to operate, all it needs is to record the performance of the ICR mice in the experiment.

As for the final huge data, Qin Lang will organize and analyze it by himself.

Hearing Qin Lang's plan, Shen Xi was also a little excited. Qin Lang was too daring to think about it, and his operability was very strong.

But the only question is whether this so-called modified ICR mouse is worth the effort to mobilize teachers and students across the school for evaluation.

If the inbred line of ICR mice is not as good as Qin Lang imagined, all the follow-up work is just a waste of time.

And this subject will become a huge laughing stock.

"Qin Lang, like this, I will seek the opinions of the other instructors on your project plan. I need to digest it. It happens that your proposal report is still some time away, so I will be here today."

Shen Xi waved his hand and let Qin Lang go out first.

Looking at the first draft of the project on the table, Professor Shen muttered to himself: "ICR mouse inbred line? This brat really dares to think. It's a headache, and I don't understand the key!"

The next day, Qin Lang was still sorting out the subject materials in the dormitory, and received a call from Shen Xi.

"Qin Lang, I basically talked to Dean Wen Yunqi about your topic and general ideas. Dean Wen expressed his support and should arrange for you to enter the laboratory of the Institute in the near future."

In addition to being the president of Zijingang First Hospital, Wen Yunqi is also the director of Zijingang Cardiovascular Disease Research Institute and the director of the Heart Center. His resume is even more gorgeous and scary:

Wen Yunqi serves as the chief scientist of the National Major Scientific Research Project (973), the vice chairman of the Cardiovascular Branch of the Huaguo Medical Association, and the chairman of the Cardiovascular Branch of the Zhejiang Medical Association; the national college president's educational system textbook "Internal Medicine" "Co-editor-in-chief, editor-in-chief of "China Emergency Medicine", deputy editor-in-chief of "China Journal of Cardiovascular Diseases"; visiting professor at Reagan Medical Center, University of California, Los Angeles, USA.

Dean Wen has a very high academic influence in the interventional therapy of heart valve disease and the basic and clinical research of stem cell therapy for cardiac insufficiency. As the first finisher, he won 1 first prize of National Science and Technology Progress Award and 2 first prizes of Provincial Science and Technology Award.

Shenxi continued to say to Qin Lang: "Qin Lang, Dean Wen still has a very good impression of you. You performed the operation on his aunt Ms. Wen Xia before. We just took this opportunity to meet Dean Wen together. "

Of course, Qin Lang was very happy to meet Dean Wen, the elder. If he could gain Dean Wen's favor and support, then the road would be smooth sailing.

Soon, Shenxi brought Qin Lang to the office of Dean Wen, and saw a thin middle-aged man with grayish temples and brown eyes, busy working on the desk, it was Wen Yunqi .

"Dean Wen, hello."

After Qin Lang said hello, she was arranged by Shen Xi to sit beside her.

Dean Wen is both fond of and curious about Qin Lang. After all, he is a junior valued by Wu Jizong, and he is kind to himself. In the eyes of the department directors of Zijingang First Hospital, he is also an outstanding junior worthy of admiration.

In particular, he dared to propose such a "daring" subject, and he was really a student full of spiritual energy.

Although Zijingang University is already ranked among the top five universities in China, sometimes many students feel that Wen Yunqi is too good, lacking pioneering spirit and adventurous spirit, and can become the mainstay of the industry, but lacks the kind of pioneering top talent.

Sometimes, Wen Yunqi is also reflecting on whether this is the tragedy of the Huaguo education system.

In many ordinary colleges and universities, even some 211 colleges, many students in the undergraduate or master's stage, do a large number of graduation thesis, there is no shortage of copy and paste, Chinese translation, and even more outrageous, first translate the article through Youdao dictionary first into English, and then re-translated back to Chinese, after a little adjustment, it is handed in.

As for the research field of the subject, basically, nine out of ten subjects are directly fed by the tutor, and it is rare to propose a research subject by himself.

Therefore, seeing Qin Lang's research project, he boldly proposed the concept of the ICR mouse inbred line, which made Wen Yunqi's eyes light up. As for Qin Lang's idea of ​​involving all teachers and students in the school, he is very much appreciated, regardless of the final result of the project. However, this process is very beneficial for cultivating and encouraging students' scientific thinking and bold innovation.

As for Qin Lang's proposal to use the laboratory of tumor prevention and treatment Zhongxi to conduct the first induction of mice, for him, the principal, it is not a matter of one sentence:

"Professor Shen, after I have considered the subject of Dr. Qin, there is no problem at all. You can arrange the opening report according to the normal time node, and I will attend after that. At the same time, Dr. Qin entered the laboratory in advance to perform ICR. Induction of mice, I approved."

"Really, that's a big thank you to the Dean."

Qin Lang did not expect that Dean Wen agreed so happily.

"Good job."

Wen Yunqi encouraged Qin Lang again, and then hurried to attend a meeting.

Shen Xi also specially instructed the laboratory on the precautions for this. As for the rest of the laboratory's regulations, of course, Dr. Wang Rou will help.

...

...

With the authorization of Dean Wen and the arrangement of Dean Wen, Qin Lang entered the laboratory the next day.

The laboratories of the First Affiliated Hospital of Zijingang University are all nationally advanced and equipped with various equipment.

At Qin Lang's request, Wu Qingguo also cooperated very well in sending the first batch of conventional Kunming mice that Qin Lang needed to the laboratory.

Although Qin Lang already had the best induction formula through the new drug research and development laboratory, as a real experiment, in order to be convincing and credible, Qin Lang still designed a control group and an experiment according to the normal process. groups, and a statistical analysis model was established.

As for the equipment, Qin Lang also prepared the NTS-1300 constant temperature oscillating water tank from Tokyo RIKEN Co., Ltd. and the CX-41 optical microscope from Olympus in advance with the help of Wang Rou.

As for the reagents, Qin Lang also strictly followed the test procedures and made detailed records. Obviously, this was a huge workload.

Of course, these reagents and materials were all paid for by Qin Lang. Looking at the small balance, Qin Lang also sighed with emotion. Sure enough, doing scientific research is a matter of burning money. Fortunately, he already has a mature plan. As for detours.

Shen Xi just allowed him to use various laboratory equipment.

After all, Qin Lang's project has not yet started. According to Qin Lang's own thinking, there is no project funding. Professor Shen also persuaded Qin Lang to apply for funding first, but he refused.

After all, for the sake of his own big plan, Qin Lang was willing to give up the money, even though it hurts a lot!

Qin Lang suddenly wanted to do some part-time plastic surgeries again. Speaking of which, he was busy recently, and only occasionally had time to go to Shuguang Beauty Plastic Surgery Hospital, but after word of mouth, there was no shortage of customers at all.

Instead, some particularly high-quality customers were selected.

Qin Lang's current plastic surgery techniques are so innocent, double eyelid surgery, and rhinoplasty surgery are all with low risk and high returns, especially if he has basic skills at the textbook level. As long as Qin Lang speaks out, those Internet celebrities are not all farts. Come over.

Qin Lang began to carefully prepare the solvent 0.9% sodium chloride injection, and the positive reagent CP (purity ≥1) was purchased from SigmaAldrich.

The preparation concentration is 4mg/mL; fetal bovine serum (fetalcalfserum, FBS) was purchased from the company

Potassium chloride (potassium chloride, KCl) was purchased from SigmaAldrich

Use deionized water to make up to 0.075mol/L. Giemsa dye was purchased from SigmaAldrich,

And most importantly, before staining, 1 part of Giemsa stock solution was diluted with 9 parts of 1/15mol/L phosphate buffer (1/15mol/LNa2HPO4 solution and 1/15mol/LKH2PO4 solution 1:1, pH 6.8) diluted to 10 %Giemsa application solution.

...

Looking at these reagents purchased from companies all over the world, Qin Lang felt a pain in the flesh. Fortunately, he had mastered the final optimal ratio. Otherwise, if he followed the normal experimental process, just to find the optimal ratio, Wasting a lot of reagents and time is not necessarily successful.

After preparing the reagents, Qin Lang began to take out the mice that Wu Qingguo had classified in advance.

Qin Lang controls 36 mice in each batch. These mice are 7-8 weeks old, weigh 30-32g, and all are male.

Although Qin Lang thinks these mice are not in the best condition, they are from the animal experiment base of Mingshi Pharmaceutical Group after all. These mice have passed the ethical review of the Laboratory Animal Welfare Ethics Committee of the National Center for Drug Safety Evaluation and Testing .

At the same time, he also has an animal production license, so Qin Lang's experiment is very rigorous and formal in all aspects, and can be included in the formal papers later.

After simulating so many lives, Qin Lang is still very familiar with the basic skills of experiments.

Qin Lang used the TOXSTAT2006 data statistical analysis software to control the individual differences in random grouping~www.readwn.com~.

After that, clinical symptom observation and body weight measurement were carried out strictly in accordance with the formal process.

Because it is in the real laboratory, Qin Lang can only administer the medication honestly, once a day in the morning and afternoon, and observe the cages, including whether it is dead, dying, activity status, appearance and coat, and whether there is any trauma. , feces, etc.; animals were weighed before administration, during administration and before dissection.

In the following week, Qin Lang entered a very fulfilling experiment.

Although the results are already known, Qin Lang still conducted a relationship analysis and record of the types of chromosomal aberrations at different dosage concentrations and sampling time points for the sake of a rigorous database.

During this period, Wu Qingguo also came to the laboratory every other day to send mice to Qin Lang, and also to observe the experimental process of Qin Lang curiously. All kinds of beautiful photos while breathing and in a daze are sent to Chairman Ming.

Finally, on the seventh day, Qin Lang completed the first batch of chromosomally mutagenized ICR mice's stable male parent.

Under the arrangement of Qin Lang, Wu Qingguo sent the first batch of male parents to the experimental base and began to breed the first inbred line.