Folk black technology boss

In the underground laboratory, the noise from the experimental equipment kept echoing in the empty laboratory.

Li Zhou repeated the experiment again and again, and then kept observing the production of E. coli in the culture medium.

Telomere repair enzyme, as a protease, can be synthesized by microorganisms under certain conditions.

But finding a suitable microorganism is not a random choice.

At least, five basic points must be taken into consideration before the enzymes needed for production can be utilized.

It can produce high-yield enzymes, is easy to cultivate and manage, has good enzyme stability, is conducive to the separation and purification of enzymes, and is safe and reliable.

Especially the last point is particularly important. As a drug, the cells and their metabolites used must be safe and non-toxic, will not affect production personnel and the environment, and will not have other adverse effects on the application of enzymes.

After careful consideration, Li Zhou chose E. coli as the "production machine" for telomere repair enzymes.

Li Zhou numbly used a machine to crush the E. coli cells cultured on the culture medium, and then put the crushed liquid into a centrifuge for separation and purification.

Looking at the yellow liquid spinning in the centrifuge, Li Zhou raised his eyebrows. If nothing else, the result was the same.

"Weixi, report to me promptly whether the telomere repair enzyme has reached the form I want."

"Okay, boss."

Distraught, Li Zhou kicked the experimental table hard.

Li Zhou rubbed his sore big toe, thinking in his mind, if Dahei was a female, why would there be so many things? Directly inject a shot of telomere repair enzyme to ensure that after a day or two, you will be active and youthful again.

It's a pity that Dahei doesn't live up to expectations!

etc?

Suddenly, an idea flashed through Li Zhou's mind, and it seemed...as if...he had caught something.

Dong dong dong~

Li Zhou hit his head with his fist, trying to force himself to think of what he had just thought.

Starting from the mashed E. coli cell fluid, Li Zhou recalled step by step what he was thinking about.

What is the form of telomere repair enzyme? No, Li Zhou shook his head and continued to recall.

Is Dahei female? Um? etc! ! !

Assuming that Dahei is female, then the existing telomere repair enzyme can completely work on the X chromosome telomeres in the cell!

Why does this result occur?

At this moment, Li Zhou remembered the inspiration that had just flashed through his mind.

Estrogen! If a large amount of estrogen is injected into the culture medium during the cultivation of E. coli, will there be any unexpected results?

As an activist, Li Zhou does what he says.

Instead of sitting on the ground and guessing, it is better to directly experiment and see whether it has the same impact as you guessed. You will know after the experiment by looking at the results.

"Weixi, prepare one estrogen... No! Prepare five!" Li Zhou paused and immediately changed his words.

"Okay, boss."

Soon, the robot brought five estrogen pills to Li Zhou on a plate.

Li Zhou picked up a tube of estrogen that was still smelling "white" and added it to the nutrient solution of E. coli.

After a while, Li Zhou poured all five estrogen tubes into the nutrient solution of E. coli.

Coliforms also have cycles and lifespans. E.coli reproduces one generation every 20 minutes, which means that its life cycle is only 20 minutes, but it takes 40 minutes for cells to replicate, which means that Li Zhou only needs to wait quietly for two days. The experimental results can be obtained in about an hour.

For a moment, with nothing to do, Li Zhou sat on the experimental table with a myriad of thoughts.

After a long and anxious wait, just 56 minutes after Li Zhou poured estrogen into the E. coli nutrient solution, the E. coli in the culture medium began to change.

"Boss! There is a problem with the E. coli in the culture medium! Weixi observed that a large number of E. coli are being inactivated, and the color of a very small number of E. coli colonies has changed."

Weixi's warning shocked Li Zhou. Li Zhou quickly jumped down from the experimental table and trotted to the medium containing a large amount of magnetic hormone E. coli.

I saw that on the top layer of the culture medium, there was a thin film visible to the naked eye, and on the upper right side of the culture medium, there was a group of yellow colonies!

Yes, it's yellow. Compared with the original white-yellow normal E. coli colony, the color has obviously changed.

Seeing the E. coli cells dying and the yellow E. coli colonies proliferating, Li Zhou did not dare to interrupt the process at all.

More than two hours later, it was already past six o'clock in the afternoon. Li Zhou had been in the laboratory for more than eight hours since Dahei died.

Li Zhou carefully took out the culture medium, and then poured all the liquid into the container and put it into the machine to mash it.

After pouring half of it, he suddenly stopped today. After thinking about it, Li Zhou thought it would be better to keep some new E. coli.

Who knows if this mutated E. coli can be re-cultivated?

You know, genetic mutations are not directional. Who can guarantee that the result will be the same next time.

In another hour, Li Zhou obtained the isolated and purified telomere repair enzyme.

Through ion chromatography, Li Zhou obtained the latest molecular structure of telomere repair enzyme.

Li Zhou looked at the molecular structure of telomere repair enzyme displayed on the computer and felt sad.

The amino acid that was missing from the telomere repair enzyme is now there, but there is an extra little tail at the back.

Dipeptide! Compared with the previous telomere repair enzyme, it has one more amino acid.

It seems that even God is going to take Dahei's life.

Li Zhou sighed and shook his head, saying nothing.

"Boss, we have come to this point, why don't you continue the experiment? You must know that one amino acid was missing before. Although it has no effect on the telomeres on the Y chromosome, it has no effect on the X chromosome."

Weixi's reminder stunned Li Zhou.

It seems...it's possible.

Logically speaking, all current telomere repair enzymes are defective products, because the information in Li Zhou's mind clearly states that the telomere repair enzyme developed works on both X and Y chromosome telomeres. Up.

"Weixi! Let's continue the experiment!"

Li Zhou used a syringe to draw a small amount of the obtained telomere repair enzyme, and then injected it into multiple male mayflies.

Mayflies, a common animal in the mountains, were chosen because their lives only last 24 hours. Therefore, mayflies are also called "one-day insects" because they have the shortest lifespan.

In fact, adult male mayflies generally only survive for a few hours, or at most a few days.

The performance of Mayfly exactly meets the experimental requirements, and the experimental results can be obtained in a very short time.

At around eight o'clock in the evening, Li Zhou, who had not eaten a grain of rice, had no patience to wait anymore. Li Zhou grabbed a mayfly injected with two types of telomere repair enzymes and dissected it, and then took a small amount of tissue and observed it under an electron microscope. Whether the telomeres on the chromosomes are repaired during the replication process.

The reason why Li Zhou gave Mayfly two injections was because one is a telomere repair enzyme that acts on the X chromosome, and the other is a telomere repair enzyme that acts on the Y chromosome.