Coroner Coroner

"Miyano, I want to ask you to be mine..."

"What's yours?" Miss Miyano pursed her lips nervously.

"Teacher." Lin Xinyi replied.

Miyano Shiho: "???"

But Lin Xinyi didn't notice her suddenly cold face at all, and just said to himself:

"Recently, there was a case where an old bloodstain that had been placed for 12 years needed to be examined."

"Kesouyan's identification technology is backward, and there is no way to extract valid DNA samples from old paper objects."

"I myself don't know much about DNA extraction and testing techniques, but you are a genius biologist."

"I hope you can be my teacher and help me study—"

"How to improve the existing DNA extraction method to extract DNA from old bloodstains?"

Miyano Shiho: "..."

The atmosphere is all up...

Is now the time to talk about research?

Speaking of which... last time too.

Last time he used the excuse of having dinner to accompany him back to the company, making the atmosphere so warm and touching.

At that time, I thought he was going to take the opportunity to say something, but I didn't expect...

That guy actually pulled her and asked about the toxicology and mechanism of action of tetrodotoxin.

Why is your EQ lower than mine...Lin Xinyi.

Miyano Shiho has been out of tune with his peers since he was a child because of his high IQ. He has always done his own way and doesn't care about the world at all.

But now she finally knows how tiring it is to deal with a person who doesn't understand the world.

"Miyano, Miyano?"

Lin Xinyi thought Miyano Shiho was in a daze.

But Miyano Shiho sighed helplessly:

How could anyone risk disobeying Gin's orders just to ask her a scientific question...

This is too... wait...

Miyano Shiho, who was about to complain about Lin Shinichi in his heart, suddenly realized the innocence of this man:

"So that's how it is... He wanted to take advantage of this opportunity to ask someone to help with the research on DNA extraction technology, so he came here to see me."

"Afterwards, Gin will be held accountable, and there will be a high-sounding excuse."

"However, this is really stupid..."

"Gin Jiu wouldn't believe that he came here simply to exchange work."

"Because I want to fall in love, has my IQ dropped?"

Miyano Shiho ruthlessly complained about Lin Xinyi's mentally handicapped behavior.

But at the same time, she couldn't help but think of the last time she was in the Japanese grocery store, she acted like a brainless star chasing girl...

Well, half a catty.

While thinking wildly, Miyano Shiho diverted part of his attention, and casually picked up Lin Xinyi's words:

"Extract the DNA of the 12-year-old blood stain on the paper object..."

"I haven't done research on extracting DNA from bloodstains, but you can try."

DNA extraction technology is necessary for forensics, especially for scholars engaged in biomedicine and genetic engineering.

Therefore, Miyano Shiho really understands better than Lin Shinichi.

As soon as she opened her mouth, she asked the key question:

"Lin, what kind of DNA extraction method does Kesouyan generally use?"

"Salting out method." Lin Xinyi replied.

"The salting-out method... is indeed prone to failure."

"After the blood has been left for a long time, the red and white blood cells will definitely be lysed to a certain extent. The formed fragments contain a lot of fat, protein and other biomacromolecular impurities, and they are distributed in uneven lumps."

"If the traditional salting-out method is used, after the protein precipitation solution is added, the DNA cannot be completely dissociated from the protein and precipitated together with the protein, which will cause a part of the loss."

"Ah."

Lin Xin nodded and added:

"So I went to Kesouyan and asked them to use the Chelex-100 method for DNA extraction instead."

Chelex can chelate polyvalent metal ions, such as magnesium ions.Just like a magnet attracts iron, magnesium ions are also attracted and connected.After the magnesium ions are removed from the reaction system, the nucleases that degrade DNA will be inactivated, thereby protecting the DNA molecules.

"This method has a high DNA recovery rate and is more suitable for extracting DNA from trace samples."

"I thought it would be better to use this method to extract old bloodstains, but it still failed."

"This method also failed?"

Miyano Shiho thought for a while and asked:

"How many STR loci were detected?"

According to the DNA identification standard, the sex loci and 15 STR loci were detected in all autosomes, a total of 16 positions, and it was considered a success.

"The Chelex-100 method is much better than the salting-out method, and 7 loci were successfully detected."

"But it's not enough for DNA testing."

Lin Xinyi replied with some helplessness:

"Other appraisers have nothing to do, and I am the same. I just know how to operate, but I don't know how to improve."

"So, I can only come to ask you for advice."

"Miyano, what can you do?"

"It's very simple." There was a sense of relief in Miyano Shiho's calm tone.

In fact, this knowledge is only the foundation of the foundation for a genius scientist like her, but being able to show it in front of Lin Xinyi can make her feel a rare joy.

Maybe it was because it helped Lin Xinyi.

Maybe it's because she can use her professional knowledge to save people instead of killing people.

Miyano Shiho is very relaxed now, as if he has unknowingly walked out of an invisible cage:

"Seven STR types have been detected, indicating that the Chelex-7 method can indeed successfully extract DNA fragments from old bloodstains, but the effect is not ideal."

"Since this is the case, there is no need to use a more advanced extraction method. A slight improvement to the Chelex-100 method may solve the problem."

Saying that, Miyano Shiho slightly pursed his lips, thinking deeply.

Although she has never studied the DNA extraction of old bloodstains before, her mind is extremely flexible and her knowledge is rich enough. By analogy, she can quickly come up with improved methods:

"The old bloodstains are closely combined with the carrier, and the styling components in the bloodstains are naturally not easy to dissolve."

"So, if you use the conventional Chelex-100 method, the effect of extracting DNA will definitely be greatly reduced."

"We can try to soak the sample with ammonia water first to dissolve the nuclear substances in the bloodstains."

"Then increase the concentration of Chelex-100 appropriately to enhance its chelating ability to metal ions and improve the extraction efficiency of DNA from old bloodstains."

"If you improve it like this, maybe you can get better DNA identification results."

Miyano Shiho methodically gave an improved extraction method for old bloodstains.

"He figured out a way so quickly..."

"You are really amazing, Miyano."

Hayashi Shinichi said to Miyano Shiho very gratefully:

"Thank you, you helped me a lot!"

"It's just a hands-on effort."

Miyano Shiho is not at all proud of the rudimentary knowledge he has used.

For a scientific scientist like her who can overcome the conservation of mass and energy, improving a DNA extraction method is probably a kindergarten-level difficulty.

Lin Xin spent so much effort just to ask this kind of question, it is really...

"and many more…"

"How can we really discuss science without knowing it..."

Miyano Shiho finally reacted.

Looking at the man in front of her who was concentrating on thinking, her expression became a little subtle:

This guy…

You really came to me to study biology? !

Chapter 133

Ms. Miyano was instinctively depressed.

It's like a cat that was left out by a shit-shoveling officer who is addicted to games, lying there quietly with its small hands in its mouth, and there is a faint resentment in its always cold eyes.

But Lin Xinyi still sat in front of her and concentrated on thinking. While thinking, he still didn't forget to stuff his mouth with food.

One mouth only focused on eating, but did not speak.

It's as if he came here for dinner today, not to see her.

But how is this possible?

How could anyone risk disobeying Gin's orders just to come over for a question or a meal?