I sell sweets in Konoha
Page 57
According to Junko's plan, the experiment on mice should have been conducted first.However, due to the current high tension between Konoha and Kumo ninja, the Hokage decided to skip animal experiments and start clinical human experiments directly.This medicine is so important to the third generation. Konoha Village has never been as eager for the birth of this new medicine as it is today.
And due to the special nature of this drug, the research and manufacturing of this drug is a semi-public plan.
If it can really be developed successfully, then the manufacturing method of this drug must be in the hands of Konoha for a long time.
All medical ninjas involved in the research of this new drug are only allowed to participate in one link, and no one except Junko herself has access to the completed production process.Moreover, the medical ninjas involved in the development are also strictly prohibited from discussing in private.
Junko visited these patients while waiting for penicillin culture, and none of them were in particularly good condition.But it shouldn't be too bad.
After a week, the cultured Penicillium can enter the next step, which is the formal manufacturing step.Pour the culture solution into a funnel with filter cotton, and place a temporarily closed tank with a bottom cloth and a drain port underneath.After the preliminarily filtered liquid is filled half the tank, take it out. At this time, pour half the tank of rapeseed oil into it and start stirring.
This step is to separate impurities and penicillin. Penicillin is a water-soluble substance. Penicillin will dissolve in water during constant stirring.The remaining insoluble and fat-soluble substances will be taken away by the oil.
After completing this step, collect this part of water from the drainage port under the tank to enter the next refining stage.
Refining relies on activated carbon. High-temperature sterilized activated carbon powder is mixed with the collected water and put into a tank for secondary stirring.During the stirring process, the activated carbon will adsorb penicillin to achieve the purpose of refining.
After completing this step, transfer this part of the mixture to the separation column and wash it with distilled water.
After completing the above steps, continue to add acidic water to the separation column. This can be made with vinegar.Since penicillin itself is acidic, the two do not resist or dissolve each other.
Finally, the alkaline water is poured into the separation column. The alkaline water is made from the juice boiled from seaweed.At this time, penicillin can be separated from the activated carbon. At this time, a funnel with filter cotton is added to the lower outlet to control the outflow speed.
In this way, a relatively high concentration of penicillin solution can be produced.
The reason why the flow rate needs to be controlled is because the concentration of penicillin will change during the preparation process.So divide the prepared penicillin into 100CC small cups and label them.
Next, the final drug test will be carried out. The staphylococci collected from the patient will be cultured on the agar medium of the petri dish, and penicillin from different small cups will be coated for culture observation.
This takes a few days, and after completing this final step all that's left is to leave it to fate.
This is the knowledge Junko read from a comic about doctors, which can then be further purified through filter paper chromatography.But Junko still doesn't know how to do this.
Although doctor-related knowledge and skills can also be purchased through the system,...
But this is not a problem that one, two hundred taels can solve!
This knowledge is expensive!Very expensive!This is really thousands of taels!
Now, the only thing Junko can do is wait.
I hope this thing can be effective...
Chapter 74 Kaiye Iruka
"Is it finished?" Sandai looked at Junko with a serious face and said, "Are you confident?"
"Well...theoretically it should be effective." Junko became nervous now, after all, she saw it in the comics.If there was a problem with any step in the process, or if she left any key steps behind, then all her previous efforts would have been wasted.Although when I gave the guarantee, it was all kinds of confidence.
But now that the results are coming, I'm a little worried.
Because although penicillin can be prepared through this indigenous method, the purity and concentration cannot be well controlled, the output is extremely limited, and the storage time is not long.
It may work, or it may not work at all.
"Let's go and take a look." The third generation is also worried. After waiting for so long, will he really be able to see the birth of this secret medicine called 'penicillin'?If this thing really exists, what kind of magical new drug will it be?And how will the birth of such new drugs affect the entire ninja world?
The Petri dishes of Staphylococcus aureus were divided into twelve groups, and each group contained the corresponding prepared penicillin.
However, since the concentration of each group is different, not every group will have an effect.Among these twelve groups, only one or two groups will have obvious medicinal effects, while the rest may not have any obvious effects.
Now it's finally time to turn on the inspection.
Junko suddenly felt as nervous as if she were holding a lottery ticket. After working hard for so long, she just wanted to see if she could win the prize.Maybe one or two of them will work, or maybe they won't.
"The first group, no reaction at all." The medical ninja in charge of the inspection started the first group of petri dish inspections.
No reaction at all.No one here spoke, disappointed?Maybe a little, but I'm still looking forward to some reaction from the next group.After all, everyone hopes to witness the birth of what may be the most important drug.
"The second group, there was no reaction at all."
"The third group didn't respond at all."
"The fourth group, there was no reaction at all."
As the medical team examined her, Junko's heart gradually sank to the bottom.Could it be... could it be said that there really is no way to use soil-based penicillin?Or did she make a mistake?Is it because the concentration is not enough or there are other reasons.
With the sixth set of tests still showing no response, Junko's heart really began to despair.
"It's okay." Sandai seemed to notice Junko's nervousness and anxiety. He touched Junko's head, and said calmly, with stability and trust: "I believe you, there must be a way. Even if everything fails this time, we At worst, we’ll have to start over.”
The third generation is not as nervous and anxious as Junko. This is a problem for the entire ninja world. It would be great if it could be solved.
That's all it can't be cracked.I am looking forward to it but not completely expecting it. Let me take some pressure off Junko now. Maybe she will have the right idea and try more in the future.
In short, if she is willing to become a great and powerful medical ninja, now is the best time to encourage her.
If it can really be built, then the three generations will give their full support even if they spend all their private money to work for others to make money!
"There is no response from the seventh group!" the medical team continued the inspection.
The inspecting medical ninja shook his head. Sure enough, what is the success rate for a seven-year-old girl to use potions made from those rubbish?What a waste of manpower and material resources, the third generation of adults actually accompanied her to fool around.
Anyway, the next few groups are pretty much the same.
He opened the upper opaque diaphragm of the eighth group.
"Group eight...Group eight..."
His eyes immediately widened, and he stared in disbelief at the blank spot above where there was obviously no staphylococci growing there.Everyone was completely stunned!
This...is this a success? !Did Group [-] forget to culture Staphylococcus aureus?
He rubbed his eyes and looked at the petri dish carefully, and then said with a trembling voice: "The eighth group has obvious drug effects! There is no growth of Staphylococcus aureus!"
"What?!" Junko and Sandai immediately rushed forward and looked at the petri dish.
Really...really no staphylococci growing!
"Great!!!!" Junko held the petri dish in her hands, and she couldn't express the excitement in her heart.Is this true?It really worked!
"Sandaime-sama." At this moment, an ANBU suddenly appeared here and approached the third generation and said: "One of our border patrol teams was attacked, and the wounded have been sent back. They have more important information."
"But the wound infection was serious and he is now in a coma."
"Are you sure?" Sandai immediately asked seriously.
"Yes." Anbu said, "Confirm, the person has been sent to the medical class."
The third generation and Junko looked at each other and immediately made a decision.Although new drugs have not yet been tested on animals to further confirm their efficacy.But time waits for no one!
The cloud ninja was no longer ready to make a move, but had officially begun to make small moves.
Use the new drug immediately. If it is effective, it will be quickly transferred to batch production, although the output may not be very high!
Junko immediately took the penicillin corresponding to No. 11 to treat the ninja, while the others continued to check the remaining parts. From the beginning of No. [-] to the end of No. [-], there were obvious drug reactions.If someone forgot about number eight, then no matter how wrong they were, they couldn't have made so many mistakes.
The conclusion is this medicine!This thing called penicillin extracted from Penicillium really works!
Junko took the new medicine and ran to see this ninja. In fact, Junko didn't want to come.After all, it would be better to leave intravenous injections to professional doctors.
Although she also learned two skills during this period, she was not in a professional medical class after all.
But...but why did this important task fall on her head?And it seems like she has to do it?
Although Junko wanted to find someone to help her with the injection.But no one is willing to take over this job.
'Hey...' Junko sighed slightly. 'I'm just...just a seven-year-old girl? ! '
It was useless to say anything now. Junko came to the ninja with the prepared medicine and checked the ninja's condition first.Well, it was an injury to the face, and even after it was cured, it would disfigure it.And it is a wound from left to right across the bridge of the nose between the lower eyelid and the maxilla.
so...
'Iruka? !What am I doing?It's actually him? '
Junko was a little surprised. How could it be him?He actually went to the front line?So is the soul on his face left at this time?Is this a coincidence?Or is it because her existence disturbs the world line of this world?
Forget it, it doesn't matter now, Iruka saved him first and said that he is in a coma and has a high fever and the situation is not good.
Penicillin cannot be used immediately, it must be tested on the skin.
A skin test involves injecting a very small amount of penicillin under the skin and observing the reaction.If there is no redness, the patient is not allergic to penicillin.If it turns red...then she's really at her wits' end.
The absence of any allergic reaction to Iluka means it can be used.
Then just inject directly.
Junko remembered that the book said that penicillin produced by this kind of earthy method can be directly injected intravenously.Although the concentration is higher, it is still not as good as the antibiotics produced by modern biopharmaceuticals in the past.
Maybe one shot will have an effect, or maybe it will take three or four times.
Now let’s see if it can be done.
Chapter 75 A thousand miles away
"That girl really did it."
After leaving the medical class in Konoha, Jiraiya looked at the sky and couldn't help but sigh.I went to investigate the girl's life experience, but there was no result for the time being and I had to return to Konoha. As soon as I arrived at the third generation's place to return to life, I heard the third generation say that the medicine had really been created.
All those who tested the medicine in the first batch are out of danger and are in stable condition.
Whether it was Iruka with an infected wound or those ordinary civilians suffering from tuberculosis, the effect of penicillin was extremely obvious and the condition improved the next day after just one injection.This magical medicine that can be called a miracle, if Jiraiya hadn't seen it with his own eyes, he would never have believed that the source of this magical medicine was actually the moldy food that was thrown away as garbage!
No, to be more precise, it is the mold spots on moldy food.
Who could have imagined that this tiny spot of mold contained such magical power?Anyway, the medical team is on their knees. Those diseases that once made them despair and helpless and were called terminal diseases are no longer a problem at all now. One shot is not effective?Then give it another shot!
"Hey...this girl is really a genius."
Jiraiya sighed slightly and was also happy. Konoha Village is really full of geniuses and Tsunade really has a successor.But Jiraiya is also worried that at such an age, he is already showing off his talents.Magical power, knowledge given by blood and soul, and political vision and thoughts far beyond his age.This may not be a good thing for a child who is only seven years old.
Her future is really bright but at the same time it is also perilous.
Jiraiya planned to visit again, to see this girl who looked very much like Tsunade when she was a child, and who was suspected of being Tsunade's illegitimate daughter with Senju bloodline.The girl's address, Jiraiya knew where it was even though he had never been there before.The place where that girl lives temporarily, but I heard that the girl is also very good at making snacks.His craftsmanship is one of the best in Konoha!
Jiraiya had only eaten a few peppermint candies, and the old man in Sandaime thought he was being stingy when he only gave him one to try out despite having a bag full of them.
Sigh... I wonder if any girl would entertain herself with snacks.
I'm really looking forward to it a little bit.
Jiraiya soon followed Junko to the hotel where he was staying. Before he even entered, there was an unbearable smell.In an instant, Jiraiya retreated three feet, and he was so embarrassed!This...what does this smell like? !Was it that girl who vacated it?Is she working on gas bombs?What are you still doing? !
Jiraiya covered his nose and wanted to leave, but after thinking about it he decided to go and have a look.
Maybe this girl is cooking up some weird snack.
And there are things in this world that smell strange but taste delicious.For example, this is the case with a strange fruit like durian.
Although he wanted to turn around and leave, Jiraiya still mustered up the courage to jump to the wall of the shop.
As soon as he jumped up, he noticed that this girl seemed to be baking a strange furry little square on the iron plate, while the shop owner was watching from a distance behind the door and did not dare to come over.
'Uh...' Jiraiya really couldn't stand the strange smell, so he jumped down and knelt down in front of Junko with his hands folded across his chest, looking at the thing on the iron plate carefully and said, "This... is. ..Tofu?!"
"Scared~" Junko was so frightened by Jiraiya that she almost knocked over the ingredients in her hand.
"Asshole! Jiraiya-sama, did you climb over the wall?" Junko complained unhappily: "Scaring people will scare them to death. Don't do this, okay?!"
"You little girl, you don't have to say that to me." Jiraiya looked at the tofu and the soft white tofu next to you and said, "What are you doing? Are you making medicine? The tofu next to this one is also moldy? oh."
"This is called hairy tofu, do you want to try it?" Junko narrowed her eyes and said with a wicked smile.
"Is this... edible? Is it moldy?!" Jiraiya really didn't know what to say and said, "Aren't you afraid of spoiling your stomach? You girl."
"Humph~ Of course I can eat it." Junko picked up a piece of fried tofu, smeared some hot sauce on it, sprinkled it with a little chopped green onion, put it into his mouth in front of Jiraiya, and said happily while eating it. : "Otherwise, what do you think? What am I doing? Although it is indeed moldy, this mold does not mean that the ingredients are spoiled, but that they are just good enough to eat!"
Hairy tofu, this is the first Chinese snack made by Junko that is not European at all and has nothing to do with Japanese.
The reason why Junko made edamame tofu was because she accidentally discovered edamame tofu among a lot of moldy food that was delivered!Although it looks like something that was born completely accidentally, it is indeed hairy tofu.It is estimated that it was not specially made but was made by accident, and was simply sent to Junko as rotten and moldy food.
However, although it is true that it is hairy tofu, because it was an accident, the fermentation was not controlled well and it cannot be eaten.
But this is the environment of Konoha Village where you can actually make hairy tofu!
After having this thing, Junko remembered that she could actually try making tofu. District 11's attitude towards tofu is still okay.Especially in District 11, there is a modified Sichuan dish that is very popular, and that is Mapo Tofu.He was so popular that a certain priest tied him to him.
But there are actually many ways to eat and prepare tofu, and the so-called Mapo Tofu is just one of them.
According to the production principle of tofu itself, tofu can be divided into different types of branches from its original form. What Junko makes now is hairy tofu.
According to legend, it is named after stinky tofu.
Originally, Junko had no intention of inviting others to eat this thing, and the taste of this thing was a bit unique.Seeing that the store manager was hiding far away, he wanted to stop Junko but he didn't dare to come forward because of Junko's uncertain identity. You can tell from his attitude of being angry but not speaking out.
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