godfather of surgery

Chapter 1067: Take One Scoop from Three Thousand Weak Waters

Sisi's physical recovery was good. After evaluation, Yang Ping felt that it was possible to use the uncastrated virus for experiments. Although there were certain risks, he decided to give it a try.

If you always stand still and don't take risks, it will be difficult to make some breakthroughs. It is feasible to take risks after making sufficient assessment and preparation.

Even if it fails, Yang Ping will treat it as a serious cold and actively prepare to continue the experiment.

The laboratory of Nandu Medical University began a new round of various tests on the K virus. After repeated tests, the new K virus was considered to have not deviated from the basic characteristics of the source virus, and the safety of using replicable viruses for experiments can be guaranteed.

The tumor laboratory at Sanbo Hospital also concluded through a series of tests that the K virus currently infects mostly tumor cells and a small number of normal cells, but from the observation of several experiments, it only causes apoptosis of tumor cells, not normal cells. Its impact on normal cells is lower than the impact of its source virus, adenovirus, on human cells.

The vector source virus used by the K virus is an adenovirus - but not an ordinary adenovirus, but a recombinant adenovirus. Recombinant adenovirus, a replication-defective adenovirus vector system, is widely used in gene therapy, basic life science research and other fields.

Recombinant adenovirus has the following significant advantages: wide infection range, can infect almost all cell lines, primary cells and some tissues; infection efficiency is as high as 100%, which is far superior to other viral vector tools and liposome transfection; large capacity for exogenous genes, up to 8Kb; no genome integration; high titer, easy operation. Therefore, recombinant adenovirus has always been considered the most promising gene delivery tool.

Of course, Yang Ping borrowed it not to let it deliver genes, but to let it deliver K factor. Before using it, Yang Ping further improved it and then implanted K factor into its protein structure to form a protein complex.

The commonly used adenovirus vectors are based on human adenovirus type 5, whose genome is a 36Kb long linear double-stranded DNA. This adenovirus is internalized into the cell through its own fibers and binding to receptors on the cell surface (such as CAR), and then transferred from the endosome to the cytoplasm and nucleus. With the help of the cell's transcription and translation machinery, the virus replication and assembly are initiated. A complete viral life cycle will trigger cell death and release viral particles.

Ordinary adenovirus is not a good thing either. Once infected, this virus can easily cause diseases in the respiratory tract, digestive tract, urinary system and other parts of the body, such as pharyngitis, pneumonia, conjunctivitis, etc., causing symptoms such as fever, cough and sputum, redness and swelling of the throat, diarrhea, vomiting, etc. In severe cases, it can also cause breathing difficulties and suffocation and death.

However, the recombinant adenovirus is completely different from the ordinary adenovirus. The difference between them is similar to that between dogs and wolves. The recombinant adenovirus has been completely tamed by scientific researchers. Not only has its replication gene been knocked out, but on the basis of mastering its genes and characteristics, some of its pathogenic genes have also been knocked out. Therefore, the recombinant virus is no longer very pathogenic, but it retains some other characteristics that are useful for experiments, such as: a wide range of infection and high infection efficiency. Moreover, after Yang Ping's modification, its infection rate for incompletely differentiated tumor cells is very high, but its infection rate for normal cells is lower than that of ordinary viruses.

Therefore, the adenovirus carefully reorganized by Yang Ping has great potential. It is a major innovation in itself. It can not only be used in K virus therapy, but also in many future tumor treatments. It is equivalent to adding a high-energy and high-precision weapon to the arsenal of tumor treatment.

Despite this, Yang Ping did not dare to take it lightly. He still carefully re-examined the genes of these viruses to confirm that there was no problem with their genomes.

This special recombinant adenovirus has another huge advantage over the original ordinary adenovirus.

The original ordinary adenovirus is highly contagious because it can survive for 56 minutes in an environment of 30°C and for two minutes in an environment of 100°C, which provides conditions for its contagiousness.

The adenovirus recombined by Yang Ping's team can survive for no more than one minute in a dry environment, with an average survival time of just over twenty seconds. Even in a humid environment, the survival time at 56°C is no more than one minute. Therefore, this virus is extremely fragile. It cannot survive in an out-of-body environment. It can only exist inside the human body, and the human immune system has sufficient lethality to it, which makes it far less threatening to the human body than ordinary adenoviruses.

So no matter from which perspective, this recombinant adenovirus is very safe, so Yang Ping’s adventure is not a random one, he will only do this when he is sure enough.
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The K virus cultivated by the Virology Laboratory of Nandu Medical University has been packaged and sent to Sanbo Hospital using cold chain transportation. Yang Ping injected the virus into Sisi's body.

It is obvious that the live virus has a much greater impact on the human body than the castrated virus. On the third day after the virus injection, Sisi developed a high fever again, but this time the temperature was not so exaggerated, only 3 degrees. For Sisi, who has experienced many high fevers of 39 degrees, he can completely withstand a high fever of 42 degrees.

This time the fever lasted longer than before, but fortunately, it fluctuated around 39.0 degrees, and the highest did not exceed 40 degrees, and the lowest was about 38.5 degrees. Its fever pattern was wavy, which was consistent with the situation of the K virus fighting the immune system in the body, because the current K virus was completely different from the previous K virus. The previous K virus knocked out the replication gene, and it was a castration virus. Under the attack of the immune system, its number would only continue to decrease, so once the high fever passed the peak, it could only go downhill.

The current K virus has the ability to replicate. After the human immune system kills some of the viruses, it will restore the number of viruses through replication. In this way, the virus and the human immune system are in a tug-of-war, and this tug-of-war manifests itself in a wave-like manner in terms of fever.

The tumor biopsy specimens sent for examination through puncture were indeed consistent with Yang Ping's speculation. They were obviously different from the previous experiments. The rate of virus infection of tumor cells in this experiment was very high. From the observation of tumor cell specimens obtained through puncture, it was found that more than 10% of the tumor cells were infected, and this rate was still rising with the replication of the virus. The specimens taken through punctures at several different parts were extremely balanced.

Due to the dangerousness of intracranial tumors, it is impossible to perform puncture to obtain specimens. However, judging from the infection rates of tumor tissue specimens in other parts of the body, the possibility of intracranial tumors being infected by the virus is almost the same, because the modified virus can penetrate the blood-brain barrier.

It was this result that made Yang Ping very excited. It showed that after being attacked by the human immune system, a part of the K virus successfully reached the tumor cells. After reaching the destination, the K virus began to use the nutrients of the tumor to replicate.

I didn't expect that such a cautious adventure would yield such a big harvest. It seems that stability is not always a good thing. Innovation must be adventurous. With continued observation, these infected tumor cells are now beginning to apoptosis on a large scale. From the calculated ratio, more than 3% of the tumors have died. The ratio of infection rate to apoptosis remains the same as before, maintaining a ratio of three to one. Because the increase in infection rate directly leads to an increase in apoptosis rate.

What does a 3% apoptosis rate mean? This is definitely faster than the growth rate of the tumor. If the treatment continues like this, the tumor may be cured. I didn't expect that replacing the strict virus with a live virus treatment would bring such a good effect.

When the experiment reached this stage, Yang Ping also began to think about naming this K virus. In fact, after many modifications, it could no longer be a virus, and it had to be given a new name - protein complex factor targeting apoptosis of incompletely differentiated cells. Yang Ping wrote this name on the whiteboard in his office.

Looking at this high-sounding name, Yang Ping leaned back in his office chair to take a rest. He hadn't been home for a few days, so now he should go back and hug his son, and of course, hug his wife as well.
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Each time Sisi's heart examination results have improved compared to the previous ones, which means that the tumor is continuing to shrink. The improvement in heart function means that the impact of the intracranial tumor on the brainstem is decreasing. This visible change is more reliable than experimental data. After all, any data in clinical medicine must be converted into clinical changes to be of practical significance.

After this virus injection, apart from a few days of fever, no other adverse symptoms appeared, which once again increased the confidence of the experimental team.

Recalling the beginning of the experiment, when we stumbled a lot, but now everything is under control, we can't help but feel deeply moved. Professor Yang is really amazing, his brain is like an alien.

K factor, recombinant adenovirus, the combination of the two, direct clinical trials, using replicable viruses for experiments, classifying this virus and removing it from the list of viruses, believing that it should be a replicable protein complex with K factor that can initiate the apoptosis program of tumor cells. Each of these is an astonishing and amazing innovation.

His experiment seemed very crude at the beginning, completely a fantasy, and seemed too far-fetched. At that time, researchers at the Virus Laboratory of Nandu Medical University even discussed privately that this idea was impossible to achieve, as it was too far-fetched.

However, as the experiment progressed, it became clearer and clearer, and the possibility of realization became greater, and slowly we actually succeeded. The whole process felt a little incredible.

They were also surprised by Yang Ping's calmness and rationality in the face of failure. Most people would be somewhat devastated after failure, but he was not at all. He was still full of passion after the failure, as if he had already determined that he would succeed.

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Yang Ping himself didn't know why he had this talent. He could always maintain a strong passion and curiosity, and accurately grasp the research direction by combining intuition and logic. Perhaps it was the result of long-term devilish training in the system space.

After Sisi's MRI image came out again, Yang Ping measured the tumor volume, especially the tumor inside the brain. Not only did it not continue to grow, but it was also shrinking compared to before. Although the reduction was very small, it was much greater than the previous measurement.

The data is consistent with the clinical symptoms, and now we can proceed with the follow-up experiments. As for ethical issues, there is actually a logic of success and failure. When you fail, you will be criticized. When you succeed, others will only see your results and forget your process.

While making rounds, Yang Ping paid special attention to the cactus in the corner. It was growing greener and stronger, and Sisi was in much better spirits than before.
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Fujiwara Masao had a certain friendship with Director Han before. He wrote a letter to Director Han, hoping that he could help arrange for Fujiwara Miyuki to come to China for long-term training for about three years.

Miyuki had studied here for a short period of time before and felt very good about it, so now she wants to study here for a longer period of time, hoping to learn Professor Yang's superb medical skills and contribute to the health of the Japanese people in the future.

After reading the letter, Director Han thought to himself that Fujiwara Miyuki met the requirements in terms of Chinese proficiency and other aspects, and mutual learning is normal communication internationally.

But now Director Han is not familiar with Yang Ping's situation. Yang Ping has long been an independent research institute academic leader, and his current job is just to provide logistical services. Director Han told Yang Ping about this and let Yang Ping make his own decision.

Yang Ping thought about it and decided that those who came to study at this time, whether from China or abroad, must be treated with caution. He passed the matter to Manager Qiu.

At present, the entire security work around Yang Ping is in the charge of Manager Qiu. Manager Qiu is extremely vigilant about Fujiwara Miyuki's visit. He thinks it is likely related to Yang Ping's latest research results. Because Huang Jiacai has told him in advance the importance of Professor Yang's research results, it may shake the fundamental interests of many pharmaceutical giants. These people either think of ways to recruit Yang Ping or rack their brains to steal the research results. If neither of the above can be done, they may take risks and use extreme means to stop Yang Ping's research. Bloodthirsty capital can do anything when it goes crazy. Manager Qiu immediately reported the matter to his superiors, who replied: You can let her come, and you must make good use of her to grasp the other party's deployment as soon as possible.

Before coming, Fujiwara Miyuki contacted Xiao Su and Tang Fei. When she was studying in China, the three of them had a very good relationship and were good sisters. They once traveled to Hangzhou together. This time when she came back, she said hello to her good sisters in advance.

Yang Ping felt no waves in his heart about Mixue's return. When it came to issues between men and women, his thoughts were simple - he only wanted to take one scoop from the vast sea of ​​​​water. He now had a wife and children, so it was impossible for him to make mistakes in this regard.

So whether she came or not, Yang Ping didn't have much to say. Whether to agree to her coming was left to Manager Qiu to decide.