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Chapter 187 Monopolized Technology

After Jiao Zhengde learned that Bai Ye wanted a high blood pressure model, he called a man. Ten minutes later, a 30-year-old man with glasses walked in.

"Teacher, what do you want me to do?" the man asked.

Jiao Zhengde introduced: "This is Bai Ye. During this period of time, he is going to learn modeling here. You should take him to study hard."

"This is Dong Cunguang, my student. He has made some achievements in the field of mouse modeling research. If you have any questions, just ask."

Bai Ye's laboratory is arranged next to Dong Cunguang, so it is convenient to take care of anything.

Dong Cunguang is a relatively orthodox person, and he wears glasses that are straight and upright. Although he is serious with Bai Ye, he responds to every request.

In the following days, instead of making models by himself, Bai Ye studied with Dong Cunguang and read books in private.

What Zhang Hanlin needs are two kinds of mice, one is essential hypertension mice, and the other is renal hypertension. His main research is the clinical research efficacy of essential hypertension and secondary renal hypertension .

Dong Cunguang is cold-hearted and warm-hearted, maybe it's not that he's cold, but that he's not good at communicating. Bai Ye only realized how extraordinary he was during the time he was in contact with him.

At the age of 31 this year, he has published 8 SCI papers. His project: Improved Modeling of Renal Hypertension in Rats won the second prize of the Kyoto City Youth Research Progress Award.

But Dong Cunguang's four children, he is the eldest, can no longer afford to study.

It just so happened that Tang Ya offered him an annual salary of one million. Dong Cunguang directly joined the company without the slightest hesitation, but the money was not in vain. The several modeling methods he improved successively brought a lot of benefits to the company.

In the past few days, Dong Cunguang is still making models for rats and observing changes in blood pressure. This is already his daily life, and the two have gradually become familiar with each other.

Bai Ye would go early every day to prepare the laboratory, and even take the initiative to undertake some things within his ability.

Dong Cunguang also gradually began to communicate more: "Why don't you try it yourself?"

Bai Ye was taken aback for a moment, and quickly nodded happily. After a few days of observation, he was a little impatient to start.

Dong Cunguang stared at Bai Ye and said, "I've been making a model of renal hypertension for the past few days, and you probably have an impression. Come and try it today, and I'll point out any problems."

Bai Ye found a relatively large male SD rat, weighing about 180g, and first shaved it with scissors, and then disinfected it with iodophor.

Modeling mice with renal hypertension is actually very simple. In fact, the renal artery is clamped with an arterial clip. The Gold-blatt hypertension model has a double kidney and a single kidney model. The double kidney is to keep both kidneys, but give one or two Renal artery stenosis, also known as two kidneys and one clip or two kidneys and two clips, the single kidney model is to remove one kidney and keep the renal artery stenosis, that is, one kidney and one clip.

One clip with two kidneys is currently the most widely used, classic, and highly recognized method of making a hypertension model in the world. It is simple to make, has a high success rate, and has a low mortality rate.

Bai Ye made a longitudinal incision 1cm to the left of the abdomen of the rat, 3-4cm long, and used 10% chloral hydrate for anesthesia, 180ml for a body weight of 1g, intraperitoneal administration...

But to Bai Ye's embarrassment, the mouse died...

Dong Cunguang looked at the embarrassed Bai Ye, and said quietly: "He is already dead, there is no need to cut him anymore."

Bai Ye didn't feel much at first, but after listening to Dong Cunguang's words, he actually felt guilty of whipping a corpse...

Dong Cunguang said lightly: "The success rate of this kind of modeling is very high, and the mortality rate is very low, less than [-]%. The most likely problem is anesthesia. When you are under anesthesia, if I guess correctly, where you injected Probably the heart."

Bai Ye: "..."

………………

But everything is difficult at the beginning, after a few days of study, Bai Ye has gradually been able to complete the mouse model independently, and the success rate is very high.

After ten days, Bai Ye made nearly 100, while Dong Cunguang made 500. This is just what he did casually...

These days, Bai Ye and Dong Cunguang have gradually become familiar with each other.

Bai Ye thought of something, so he asked, "What do you think about the modeling of essential hypertension?"

Dong Cunguang pondered for a moment, then stopped what he was doing: "It's not easy to do, mainly because the results lack uniformity and individual differences are relatively large. The cause of essential hypertension is unknown, so there are many methods in the process of modeling. Such as nervous, spontaneous hypertension and so on."

When Bai Ye heard it, he suddenly thought that the new type of modeling in the experience of raising mice required the gene of high blood pressure mice, so he asked, "Is there a mouse with a high blood pressure gene?"

"Yes! There must be. At present, SHR is the most used in the world. Dr. Okamato mated male wistar mice with blood pressure of 19.3~23.3kpa with female mice of the same species with blood pressure of 17.3~18.6kpa. The offspring choose People with high blood pressure exceeding 24KPa, they call it spontaneously hypertensive mice (SHR). Through continuous selection, by 1969, the inbred line SHR was obtained, and by 1986, the line had reached 80 generations. And the technology It's very mature."

Bai Ye's eyes suddenly lit up, because in the experience of raising mice, the model of essential hypertension mice requires gene replacement with mice with the high blood pressure gene, so after knowing the SHR mice, Bai Ye was also happy: "Then What percentage of them have high blood pressure?"

Dong Cunguang: "Hypertension is 30 percent, and blood pressure generally increases with age after birth. According to statistics, in the 32th to 24.5nd generations, the average blood pressure of male rats at the tenth week is 2.3±23.7kpa, and that of female rats is about 1.9±[-]kpa. It will continue to rise thereafter.”

Speaking of this, Dong Cunguang shook his head: "The price of an SHR is about 500 in China, but the domestically made SHR is very ineffective and has a high prevalence rate. In comparison, foreign ones are more stable and the price is lower. There are only 200-300, so this kind of mouse is very expensive. Our company has also cultivated it at the beginning, but unfortunately the core technology of SHR cultivation cannot be passed on. The SHR we cultivated is far from the SHR of Longka Company. Well, if we use the recently popular BPFH ratings, the best ratings we train are at the second-class level, and the worst Ronkas are at the second-class level, generally first-class."

When Bai Ye heard this, it was no wonder that Zhang Hanlin kept buying rats from abroad. The original reason was here. Thinking of this, he couldn't help asking: "Then there are no other rats? To replace this SHR."

Dong Cunguang suddenly smiled, very rare: "Yes! But... immature, and still under study."

Bai Ye suddenly realized that this was the first time he laughed since he met Dong Cunguang, right?
"any solution?"

Dong Cunguang's eyes were full of light: "Genetic engineering, I'm currently studying the genetic mechanism sequence of SHR. If I can find the difference between him and other mice, maybe I can learn from it, so that at the embryonic level, the shotgun method can be used to change the normal embryo. Gene sequence, maybe new things can be obtained."

After Dong Cunguang finished speaking, he was full of expectations: "One day, we will break this disguised technological monopoly and let gringos buy our mice!"

(End of this chapter)