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Chapter 188 Genetic Engineering Upgrade
186:

In fact, when he came to Naya's laboratory, Bai Ye's focus was not to study the modeling of renal hypertension, but to study the model of essential hypertension through the process of modeling renal hypertension.

In Rat Raising Experience, Thacker's model of essential hypertension is a product of genetic engineering, which achieves essential hypertension by modifying a certain gene sequence.

But you can't become a big fat man with one bite, you must have a solid and stable foundation, just like your high-level martial arts cheats, but you don't have the mentality to get started.

Moreover, modeling is just a method, the real thing is that Bai Ye hopes to improve his level of genetic engineering through the process of modeling using genetic engineering.

The level of genetic engineering is high, and it is absolutely acceptable in the current environment!

The key is that this kind of modeling can also allow him to complete Zhang Hanlin's serial tasks, which is also a top priority.

Ke Ao's task has always been one of Bai Ye's thoughts. If you want to prove the instability of KM mice under drugs, and change the unstable sequence of mice to find new products, you must establish a high-level gene Based on engineering level.

The joining of Bai Ye made Dong Cunguang even more ambitious and high-spirited. Every day, the two of them held a few thick books on genetics for discussion, or did research with mouse embryonic cells.

It has to be said that Naya attaches great importance to scientific research, millions of equipment can be bought as soon as it is bought, and Dong Cunguang's laboratory is equipped with various modern scientific research equipment.

Nucleic acid molecular hybridization technology, accounting gel electrophoresis technology, DNA sequence analysis technology, gene site-directed mutagenesis technology, polymerase chain reaction technology...

These supporting books and materials are piled up in Dong Cunguang's laboratory.

Bai Ye's learning is selective learning, because when he first acquired the genetic engineering skills, activation required conditions.

[Genetic engineering lv3, requirements: basic skills: genetics: lv3, molecular biology: lv3, cell biology: lv3. 】

Dong Cunguang was originally engaged in biological engineering, and he was very accomplished in memory engineering. Under his guidance, Bai Ye made rapid progress.

In a few days, Bai Ye's genetics, molecular biology, and cell biology all reached level 3.

And Bai Ye also heard a ding:
[Scientific research skills: genetic engineering activation, current level lv3: 3900/10000. 】

Bai Ye understood that the extra 3900 experience points were the result of his studies and experiments these days.

In the next few days, Dong Cunguang received an assignment notice to attend an academic forum, leaving Bai Ye alone in the laboratory.

However, under Dong Cunguang's leadership, Bai Ye has also learned a lot about the use and operation skills of these instruments.

After this period of study, Bai Ye wanted to try the research on the new type of essential hypertensive mice.

It is recorded in "The Experience of Raising Rats" that it is necessary to compare the molecular sequence of ordinary rats with the hypertensive rats of the same species, and then obtain matching nucleic acids, and then use the shotgun method at the mutation points. It is carried out under an ultramicroscope, and the most critical thing is if there is a suitable hypertensive mouse model.

Dong Cunguang has been researching the SHR gene sequence for a long time, and based on his research, Bai Ye feels that the time is ripe.

Bai Ye extracted the DNA sequence from the embryonic cells of ordinary SD rats, analyzed them in a DNA molecular sequence analyzer, and then separated them by electrophoresis to obtain nucleic acids.

But shotgun replacement is not that simple, and many kinds of separation are very prone to problems when used under a microscope.

However, when Dong Cunguang came back, the progress of the research was a lot earlier, but not all recombinant DNA can survive, especially inbred mice like SHR, who already have essential hypertension and a variety of recessive Gene, after he combines it with ordinary mice, it is not easy to form a stable nucleic acid sequence.

Looking at the sequence diagrams of the two sets of DNA, Bai Ye said, "Actually, we may have entered a misunderstanding."

Dong Cunguang was taken aback: "What do you mean?"

Bai Ye frowned and looked at the two sets of sequences: "Maybe we can change our thinking and improve SHR instead of putting the SHR gene into other mice."

Dong Cunguang's eyes suddenly brightened: "You mean to use the stable nucleic acid structure of ordinary mice to compensate for the genetic defect of SHR?"

………………

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Three days later, the two had cultivated a batch of new embryonic cells, which were waiting to be implanted into female female mice, and then hatched to see the results.

Waiting is the longest process, but Bai Ye has gained a lot.

【Ding!Distinguished member, your scientific research skill genetic engineering level has been raised to lv4. 】

After the level reached lv4, Bai Ye felt that many complicated problems in his mind suddenly became clear. This was not only a level improvement, but a systematization and perfection of knowledge.

Bai Ye felt that the originally hazy and mysterious DNA sequence seemed to have a clue.

At this time, the new model mice had to wait for the results, and Bai Ye didn't want to continue the research.

He has a more important thing, which is the stability study of the KM mouse gene.

With the foundation of this period of time, Bai Ye felt confident.

However, as a mature and commonly used experimental mouse, it is obviously unwise to deny its stability.

However, after consulting a large amount of data, Bai Ye found that there were indeed some examples of experimental failures, and the reasons were unknown, but many of the animal experiments used in these experiments were KM mice.

In fact, why mice are used as experimental animals for human drugs is because researchers have analyzed 96% of mouse genome sequences, and 99% of the genes can find homologous sequences in human genome sequences. It has been confirmed that mice and humans are highly homologous at the gene level.

But that doesn't mean mice and humans are genetically similar.

for example:

A: 1, 2, 3, 4, 5, 6, 7, 8.

B: 1.2.3.4.5.6.7.9.10.11.12.13.14.15.16, 17, 18, 19, 20, 21, 22, 23, 24, 25...

90% of the numbers in series A can be found in series B. Do you dare to say that the similarity between the two series is very high?

In fact, the relationship between these two sequences is similar to the relationship between the mouse genome and the human genome.

99% of mouse genes can find homologous sequences in humans, but human genomes cannot be found in mice.

What Bai Ye needs to do is to prove the instability of the A sequence, and prove that the special genetic structure of the mouse will make the mouse more adaptable to the environment under certain conditions and slightly change its own gene sequence. Not suitable for pharmaceutical experimentation on humans.

How to find out the reason for the instability of the mouse gene is what Bai Ye needs to consider now.

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(End of this chapter)