I really don't want to be a doctor

Chapter 98 Drugging the Rats
morning.

Jing Xiaoran ate a drawer of steamed buns at the Little Sun Noodle Restaurant, and then brought the raw materials for the experiment to the laboratory.

Because today is Saturday, there are fewer people in the laboratory than usual.When Jing Xiaoran came to the laboratory on the second floor, there were only two girls busy with their experiments.

Now Jing Xiaoran has a detailed experimental procedure in his mind.

The production method of "new oral anticoagulant" uses p-nitroaniline as the starting raw material, and 4-(4-aminophenyl)-3-morpholinone is prepared by ring-opening, cyclization and reduction, and then added , cyclization, deprotection, condensation 4-step reaction to generate the final product.

All the operation steps are not difficult, as long as the reagents are added step by step.

In Jing Xiaoran's impression, this method has been improved many times in previous lives, and the conversion rate of the final product can reach 30-40%.

After putting the raw materials and some reagents on the experimental bench, Jing Xiaoran started the first step of the experiment, the ring formation reaction.

The production method uses 2-(2-chloroethoxy)acetyl chloride and p-nitroaniline as starting materials.

First, add p-nitroaniline to tetrahydrofuran as a solvent, use potassium carbonate to create an alkaline environment, and add 2-(2-chloroethoxy)acetyl chloride for substitution reaction to obtain an intermediate.

Add acetonitrile to the intermediate to raise the temperature and reflux. After the intermediate is completely dissolved, potassium carbonate is added to generate a ring-forming reaction.

This is the first step of the experiment.

Although it is not difficult, it is extremely important, and it is related to the success or failure of the entire experiment.

Jing Xiaoran concentrated on mixing several reagents one by one.

Generally speaking, in order to write convincing papers, the material structure of the intermediates in each step should be determined.

The determination process is somewhat cumbersome. First, the molecular weight is determined by mass spectrometry, then the elemental composition is determined by elemental analysis, and finally the molecular structure is determined by NMR.

But this experiment is different from others. Jing Xiaoran already knows what the final product is. In fact, he can directly measure the final product without going through the measurement of the intermediate.

However, just to be on the safe side, Jing Xiaoran still decided to analyze the structure of the obtained intermediate after each step of the experiment.

Although the process will be somewhat cumbersome, the papers written in this way will be more convincing, and it will be very helpful for future patent applications for medicines.

An hour has passed.

The first step of the experiment ended smoothly, and Jing Xiaoran began to analyze the substances of the intermediates, and all the instruments needed were in the laboratory on the fourth floor.

When I came to the fourth floor, there was no one in the laboratory.

The instruments for mass spectrometry and elemental analysis are idle, while the analyzers for NMR are working.

"Looks like I'll have to wait until this is over."

The waiting time was quite long, and Jing Xiaoran began to sort out the next experimental steps from the side.

"Ding ding..."

At this time, Zhou Baolin suddenly called.

"Xiao Ran, didn't you come today? I didn't find you on the second floor."

Jing Xiaoran said: "What's the matter? I'm on the fourth floor."

"It's like this. The senior sister in the group asked us to feed the mice, but she doesn't seem to be very good at it." Zhou Baolin whispered, "I just wanted to ask you if you can? If so, come down and teach Let's do it."

"Okay, I'll come down right away." Jing Xiaoran glanced at the nuclear magnetic analyzer, it would take more than ten minutes to finish, and it shouldn't take much time to go down and teach them.

Go to the lab on the first floor.

Jing Xiaoran found that there were more than ten students in the laboratory, most of whom were newly recruited first-year students.

"Xiao Ran, you are finally here." Zhou Baolin ran forward and said.

"What's going on?" Jing Xiaoran asked.

"Senior sister arranged for us to give drugs to the experimental mice." Zhou Baolin smiled wryly, "But senior sister taught it once, and then left after saying something, let us try our own hands. How could we do it!"

"And the little white mouse she drugged seemed to have been force-fed to death."

Jing Xiaoran frowned, he looked at the small white mice in the cage, there were about 30 of them.

Although it is very simple to feed the mice, the students present are novices, and some girls are even afraid to touch the mice.

Just like Luo Xin and Ji Ying standing in front of the mouse cage, they looked eager to try, but they dared not make a move.

Many people in the laboratory saw Jing Xiaoran's arrival and recognized him as the boy who gave them a meeting that day.

"Everyone, let me give it a try." Jing Xiaoran walked to the mouse cage, and the people around him consciously made room, "Whoever brought extra gloves, lend me a pair."

A boy from Class Two hurriedly took out a pair of size seven and a half gloves from his backpack.

"Thank you." Jing Xiaoran put on his gloves, opened the lid of the mouse cage, and skillfully took out a small white mouse, "I'll do it from beginning to end first, you guys watch first."

Everyone saw that the restless little white mouse suddenly became "docile" in Jing Xiaoran's hands, with surprised expressions on their faces.

Jing Xiaoran's movements in his hands were unhurried, he took out the drug-filled syringe, inserted it into the mouse's body through the mouth, and then pushed the drug in.

From picking up the little white mouse to putting the little white mouse in a free mouse cage, the whole process is only a few tens of seconds.

Everyone had a very strange feeling in their hearts. Is this process so simple?

But when I thought about the drug feeding process of the previous senior sister, it took nearly 5 minutes to catch the mice, and finally killed the mice.

This "simple" is probably an illusion.

"Next, I'll do it while I'm talking. Listen carefully." Jing Xiaoran said, "I'll start by grabbing the little white mouse."

"Um... class monitor, can I make a video?" said the boy who lent Jing Xiaoran the gloves, "I won't forget later."

"Yes." Jing Xiaoran nodded, "But all the videos in the laboratory cannot be uploaded to the Internet. I have told you about this before."

"Okay." The boy took out his phone and pointed it at Jing Xiaoran.

"Don't slap me in the face."

"Oh oh..."

Jing Xiaoran began to explain the detailed process of grabbing and pouring medicine.

"The way laboratory animals grasp is different. Just mice can be divided into mice, rats, guinea pigs and so on."

"White rats should be the simplest kind. They generally don't bite people, unless they act violently, and they may bite you."

"First lift the mouse tail with your right hand, be careful to grasp the middle or base of the mouse's tail, and never grab the tip of the tail, because the skin at the tip of the tail is easy to fall off."

Speaking of which, Jing Xiaoran pulled out a small white mouse from the mouse cage.

"You can try it yourself." Jing Xiaoran put the little white mouse back into the cage.

After hearing what Jing Xiaoran said, everyone seemed to want to try.

Zhou Baolin took the lead, walked to the mouse cage first, imitated Jing Xiaoran, put on gloves, grabbed a small white mouse by the tail, and pulled it out of the mouse cage.

"Haha, I caught it." Before Zhou Baolin finished speaking, the little white mouse in his hand broke free and fell back into the mouse cage.

"I told you, don't grab the tip of the tail." Ji Ying said from the side, "Look at the skin of the mouse's tail was torn off by you."

Zhou Baolin was a little embarrassed. He glanced at the mouse cage, and it seemed that the skin of the mouse's tail had really been torn.

(End of this chapter)