musicians of old

Chapter 185 The Occult Edition "Chromatography"

"Uh…"

Sheeran and Joan followed the direction of Fanning's finger and looked towards the end of the table.

Sink, washbasin, brush, alcohol, washing powder, steam oven...the whole set of utensils are neatly placed there.

"Okay. It's not impossible." Joan curled her lips, "But I'm mainly curious about what you want to do. Maybe you don't believe it, but methods such as distillation, crystallization, and centrifugation are really inseparable from these extraordinary components. , in very few cases, all are high-level and above wise people, relying on their own strong inspiration control power, with the help of some means to pull out the chaotic transmutation products, and this is still incomplete separation and purification."

Fanning has already started his operation.

"Stay away first. You are not wearing masks. It is difficult to expel this stuff after it is inhaled into the lungs."

He cut open the silica gel powder packaging bag, poured half of the volume into a large glass with a capacity of more than one liter, and then poured finely ground flint particles and burnt ash into it according to the volume ratio of 5% and 2%. The slag's black skeleton and light spot glands.

Flint grains are a kind of moving substance. In some dreamlands where the light and shadow are close to noon, those who know can collect a heavy and shining crystal on the surface of the dusty soil, and can experience the affinity of inspiration for it by sliding their fingers through it.

And the black bone spot is a kind of "trace spirit" in the movement. This kind of substance is considered to be "the leftovers of the glow quality and the memory of history that are constantly drifting downwards", although there are living things in the movement. characteristics, but it is far inferior to the 'moving creature', and cannot affect the waking world.

The "skin of the end", the carrier of the common spell seal, also comes from a plant-like trace spirit in Yiyong.

The dust in the air is scattered, and the silica gel after mixing treatment has the adsorption force for extraordinary substances similar to ordinary substances.

Fanning picked up a large Erlenmeyer flask with both hands and held it up to his eyes for observation.

"What is this?" Sheeran was very curious.

"Water, and 0.8% industrial cellulose that has undergone a rough carboxymethylation process." Fanning said, "You can understand it as a kind of thickening or binder that is not very easy to use and is quite expensive."

"Are you a transfer from Saint Lenia Institute of Technology?" The two girls touched their heads blankly.

"It should be about the same after two days of standing." Fanning pulled out the stopper, poured the supernatant into another glass, then added the treated silica gel powder, and stirred it into a large mass of light gray with a little transparent glitter Textured viscous slurry.

Then he took out a glass plate with a size of 10x10 cm, dug a large spoonful of slurry with a spoon and put it in the middle, tilting it left and right, so that the slurry flowed evenly on the surface under the action of gravity.

".What does this have to do with the separation of extraordinary components? Callon, are you making pastries? Can this thing be eaten?"

Seeing Fanning put the square glass plates coated with glitter slurry into the oven one after another, Joan couldn't help asking.

"Aren't you full yet?" Fanning closed the oven door, turned on the steam heater, then returned to the table, reached out and pulled a handful of cotton from nearby.

On the steel bracket in front of the wall, a long glass column has been fixed with clips. Its length is nearly one meter up and down, but its diameter is only three centimeters. There is a frosted opening on the top and a glass plug on the bottom.

Fanning threw cotton into it, gently pressed it against the bottom outlet with a long glass rod, and then spread a layer of washed river sand on top.

He unscrewed another brown bottle, poured the gray clear liquid into another part of the silica gel powder, and stirred it into a slurry.

There was a slightly rotten smell in the air.

"What is this?" Sheeran asked curiously.

"Curtane." Joan explained, "The low-boiling-point extraordinary solvent he screened out earlier is made from the trace spirit 'blight moth' in the water and some light petroleum components, and its solubility is relatively poor. It is narrower, and the background interference to spiritual perception is also weaker, and it has some applications in the crystallization of extraordinary substances."

"But I still don't understand." She couldn't help but asked again, "Caron, why don't you try to dissolve the sample with cumulane, what are you doing mixing it with silica gel? These two things are incompatible. Rong, it’s just a sticky slurry, and you made this thin and long pillar, what is it for?”

"I'm filling the 'column chromatography'." Fanning raised the large cup of slurry, drained it with a glass rod and poured it into the column, then sucked up a few pieces of cumane with a dropper, rotated it on the top of the column and extruded it to remove the residue on the wall. Silicone powder is washed down and the top is finally plugged.

A miniature steam blower attached to a rubber tube began humming, compacting the silicone slurry in the column.

The cumulane flowed out from the bottom, and he caught it with a cup and poured it into the top again. After repeated operations, a large section of silica gel column intertwined with dark and shiny contradictions appeared in the glass. A few centimeters of cumulane liquid seal.

"Column chromatography? Did you invent this? This column...can separate extraordinary components?" Joan looked closer in disbelief.

"You'll know later." Fanning smiled mysteriously.

Fan Ning filled several such pillars in order, thick and thin, and then clamped them in the air with clips, and lined them up on the steel support.

A few people wandered around for a while. In the evening, Fanning opened the oven door and took out the tray. The silicone slurry on the square glass had become dry and smooth.

He took out a glass cutter, drew straight lines at an interval of two centimeters on the back of the glass, and then "bang bang" broke it into glass strips.

Before Joan could ask, he took the initiative to explain: "This is thin-layer chromatography... I will first take an extremely small sample, use it to detect the distribution of extraordinary components in it, and then formulate a separation strategy and use column chromatography for separation. "

Whether it is column chromatography or thin layer chromatography, their separation principles are the same:

Under the scouring of the mobile phase, different substances move through the stationary phase at different speeds—the speed of movement here is strictly speaking the speed of the cycle process of "adsorption-desorption-re-adsorption-re-desorption".

In Fanning's previous life, the common stationary phase was silica gel, and the mobile phase was organic solvents such as petroleum ether/ethyl acetate/dichloromethane.In this world where mysticism is popular, the stationary phase he chooses is silica gel treated with extraordinary substances, the mobile phase is the cumulane he screened out, and the coelomia lymph fluid and silverfish instars that will be used next passivated ester.

With the column and thin-layer chromatography all ready, he finally started working on the samples.

Fan Ning's mood at the moment is still a little excited and apprehensive. Splitting out the extraordinary components in it is only a small part of the meaning. More importantly, if the chromatographic principle can be reproduced in this world, those so-called "waste formulas" will be lost in the future. After separating the deadly by-products of chaotic transformation, it will become a sky-high priced high-ranking elixir!
He put the three kinds of pigments into the separatory funnel one by one, first added water and stirred to form a cloudy suspension, then poured the same volume of coelomia lymph, shook, stood still, and layered.

This is an extraordinary solvent with strong and wide solubility. At this time, most of the mixed extraordinary components in the pigment have entered this layer. Under the spiritual perception, the dilute solution presents chaotic light and shadow with mottled colors.

Open the plug, separate the liquid, add it again, shake it and let it stand for layering...

After watching for so long, Joan finally had something she could understand, so she turned to Xilan and said, "...his extraction operation is more standard than mine, and he is the most proficient among musicians I have ever seen...I think he He must have sneaked away to study in the Chemistry Department of the Polytechnic Institute..."

The sample turned into a stock solution, and Fan Ning took out a few tiny sample tubes the size of the tip of a little finger, labeled them, and packaged them one by one for storage.

Then he picked up the graduated cylinder.

"The configuration of the mobile phase... the ratio of cumulane and coelomia lymph fluid is first tried to be 5:1, and then the passivation ester of pentadida instar is added."

After Fanning finished measuring, he poured the mixed solvent into a cylindrical glass bottle. He used a very small amount, and the depth of the liquid in the bottle was only half a centimeter.

Inside this bottle is where we will run TLC later.

He took out a piece of thin-layer chromatography that had been divided into rectangles, drew a straight line with a pencil one centimeter from the bottom of the silicone surface, marked three points on the straight line, and wrote 1, 2, 3 in small characters.

Use a capillary glass tube to gently absorb the original pigment solution of the watch factory, the poorhouse pigment solution (yellow branch), and the underground building pigment solution, and click on the positions 1, 2, and 3 on the straight line in sequence.

Finally, he picked up the piece of thin-layer chromatographic glass with tweezers, put it into a cylindrical bottle, made the straight line of the pencil above the liquid surface, and closed the bottle cap.

Just like water soaking the paper surface, the mixed Extraordinary solvent began to climb up on the silicone surface. Visible to the naked eye, a darker area was spreading from bottom to top.

A minute later, when the dark side was almost at the top of the glass, Fanning clipped it out.

The low-boiling extraordinary solvent quickly evaporated in the air, and the dark side disappeared. The silica gel on this glass plate did not look any different from before. Even Fan Ning's spiritual sense could hardly see the heterogeneity on it. light and shadow.

Because the concentration is too thin and too thin.

"What happened?" Joan and Sheeran looked blank, but when Fanning moved the glass plate to the jewel altar that was already in operation, they saw a surprised scene through the crystal ball: three "runways" , the top is the striped light and shadow of split iteration, and there are golden spots in the middle of No. 2 and No. 3!

Sure enough, as Fan Ning expected, although the concentration of Extraordinary components is too low, his spiritual perception cannot be analyzed, but he can use the form of crystal ball + altar to strengthen his spiritual perception.

Just like the way I used to observe TLC in my previous life, with ultraviolet lamps or iodine vapors...

In this mystical world, the way to observe extraordinary matter is spiritual perception + mysteries. Of course, if you want to further characterize it, you may still have to separate it and rely on the accumulation of hidden knowledge of those who know it to judge-it may be difficult to move matter. Understand as a molecule or a pure substance.

For the purpose of "revitalizing the formula of the spent elixir", it is enough to be able to observe and separate it.

Joan is also quite gifted and knowledgeable in the science of elixirs, and she immediately interpreted this phenomenon.

Sample No. 1: Pigment from the watch factory, containing only a single extraordinary component of a certain "derivative" phase.

Samples 2 and 3: Workhouse and underground building paints, in addition to the "derivative" phase component, there is also a certain "candle" phase component that was separated by Fanning!

"Caroun, what is your invention called... thin-layer chromatography? I didn't expect this method to be really useful!" Looking at the thoughtful Fan Ning, Joan's tone was very excited.

"The successful splitting and mixing of extraordinary substances is extremely rare. I didn't expect you to succeed in the first time you practiced elixirs. It was the kind that cost very low and took a short time. You are really lucky!"

"Luck?" Fanning smiled lightly, "You will know right away, my method can be reproduced in most situations."

"Change the ratio of cumulane and coelomia lymph fluid to 15:1, and add two drops of instar inactivated ester." He measured the new mobile phase ratio and poured it into a cylindrical glass bottle.

He drew a straight line on the second thin-layer chromatography, wrote down the numbers, and then used a capillary glass tube to absorb the stock solution and opened it again.

At this time, Fan Ning wrote an extra No. 4: the elixir that the principals take.

Submerge the lower end of the straight line in the cylindrical bottle in the same way, and clamp it out when the special solvent is about to climb to the top.

The next second, Joan stared at the crystal ball in the altar with a look of shock on his face.

Sample No. 1: Pigment from the watch factory, the front end is still "Yan", but it has not gone so far.

Sample No. 2 and No. 3: Pigments for workhouses and underground buildings, "Yan" also did not run that far, and the phase composition of "Zhu" basically did not "run", and still stayed at the starting position.

What is even more surprising is sample No. 4: the elixir taken by the principal, which has three kinds of light and shadow, the top is "yan", the bottom is followed by pink "pool" and green "cocoon", but they are all before On the "candle".

There are actually four extraordinary substances independently presented on this so-called "thin-layer chromatography"!

Joan yelled out excitedly. Fortunately, the third floor was empty and the sound insulation effect of the building was also very good.

Fan Ning was also vaguely excited at this moment, because he found that his guess was correct, and the principle of color spectrum was also applicable in this mystic world.

The only difference is that the speed at which ordinary substances flow through the chromatography depends on their polarity, or degree of molecular symmetry.In the positive column environment, the more symmetrical the polarity is, the faster the flow will be; otherwise, the more asymmetrical the polarity will be, the slower the flow will be.By adjusting the mixing ratio between several strongly polar and weakly polar solvents, their development can be controlled and placed in the most suitable position.

And Extraordinary substances...Fan Ning initially discovered that their flow speed seems to be related to the overall arrangement of the seven phases!Under the current extraordinary solvent configuration, the fastest expansion is "Yan", and the slowest is "Candle"... His idea is also based on the solubility of extraordinary solvents, using a combination of high and low performance solvents, and controlling the proportion Control unfolding...

After summing up the preliminary laws of mysticism in his mind, Fan Ning's brows gradually frowned.

First of all, there is the issue of "candles"...Since what he chopped off with a crowbar in the underground building happened to be a piece of yellow paint, so this time he first chose the Langefni brand paint that was also yellow. Looking at it now, they have a total of " Candle" ingredients.

Furthermore...

His eyes stared at the light and shadow of the "derivative" phases on the TLC silica gel.

Both the paint and the elixir have "derivation"?Is this a coincidence or...?
Joan is obviously also aware of this problem: "...Callon, the 'runway' of your different original liquids, and the light and shadow of the 'derived' phases all climb at the same height... Does this mean that they are the same extraordinary substance?"

"Extremely possible, but a more rigorous method of verification is needed."

In order to make "Yan" climb a little lower, Fan Ning changed the mobile phase combination method with lower solubility - cumulane: coelomia lymph fluid = 30:1, and dropped a drop of beetle instar passivation ester.

He took out the third thin-layer chromatography glass plate, and this time he ordered an additional mixing point for several samples on the basis of the previous one.

Climbing again, several people found that at this time, 'Zhu' and 'Cocoon' almost didn't run at all, they were all mixed at the starting point, 'Chi' went up a bit, and 'Yan' was in the lower middle position, and all The height of the "Yan" in the runway is still exactly the same!

"The elixir that the principals ate...contains an extremely small amount of the extraordinary component of the radioactive 'derived' phase in the paint of the Jinlangia Watch Factory!?!?" Fan Ning was dumbfounded.

The maker of the elixir, Dean Graham, a professor of chemistry at the Polytechnic Institute? …

Pigment supplier for watch factory, Rylatim Chemical Trading Company? …

The situation in the imperial capital...

Infiltration of the Consonance School…

The elusive Special Patrol Office...

Investigator driven mad by the "fountain in the painting"...

Many fragments of information immediately appeared in Fanning's mind.

Sheeran expressed deep concern: "...So, how long have you been taking this elixir, Principal Sternike and Vice-Principal Huxley?"

Fanning tried to calculate how much time had passed since the premiere of the First Symphony.

Today is already the night before departure to the imperial capital to perform!

"Maybe...have you eaten for almost two months?"

　Thank you for the reward of the book friend tail number 8 on August 20~Thank you little ghost for hitting me, Hanshao Immortal Emperor, book friend tail number 6786, decadent farewell, why do you want to name the crowd, the unicorn looking up at the starry sky, cmfu old Gong, I don’t like Jiangshan, I like mathematics, I want to dig potatoes, Xuanyoukong, Xingyun Deacon’s monthly ticket~
　　
　
(End of this chapter)