musicians of old

Chapter 186 Teaching Hard Core Column Tomography (4K [-] in [-])

Fanning's mind quickly flashed the impression of meeting with the two principals several times during this period.

Seems pretty normal.

The concentration of the extraordinary active ingredients in the "cocoon" and "pool" phases of this elixir that is taken continuously is already very low in a single dose. It's even thinner.

The "Yan" in the pigment of the watch factory is also very thin, but there is no interference from other extraordinary components, and this healing elixir. If it is not separated by TLC, the spiritual sense will look like a dark green that is blooming and churning. Didn't notice anything unusual at all.

If anything, it will be slow and invisible.

Fanning quickly opened the drawer, wrote a few words on the note paper, stuffed it into the envelope, and went downstairs to let the clerical staff deliver it to Roy first.

Although I didn't know the specific situation, I quickly suggested that they stop the drug and bring that Dean Graham under control.

Furthermore, the "derivative" phase component in the watch factory paint is the same as the paint from the Langefni Workhouse and the paint scraped from the underground building!
The investigation results of the authorities show that the labor symptoms have nothing to do with the production process? .
The results of the investigation by the Special Inspection Office showed that the person in charge of the Jinlongiya Machinery Factory subjectively did not know that there was a hidden organization infiltrating and lurking?Therefore, according to the principle of "the ignorant has no obligation to guard against mystery", the responsibility of the mysterious side is exempted?
"The authorities and the Special Inspection Office are fucking bullshit." Fan Ning sneered from the bottom of his heart.

"The Langefni pigment factory in Calone, do you want it now?" Sheeran asked after Fanning re-entered the door.

"It's getting dark." Fan Ning shook his head, "Tomorrow we have to pack up our things and rush to the imperial capital. We can only ask the Guidance School to dispatch the police and security department tomorrow, and seal that poorhouse. The factory owner Stanley , grab it and talk."

"And Julius." Joan reminded.

Fanning had told them before that Julius seemed to have become a knower.

"Her? For the time being, follow me to the imperial capital." Fan Ning, wearing a white coat, paced back and forth in the elixir room, thinking.

"From a legal point of view, Stanley is the person in charge of the workhouse and the factory, not her. The only way to deal with her is to force her or report it to the Special Inspection Office under the pretext of violating taboos. This seems to be impossible for us. Bring very clear benefits, or track down the clues of the experience officer 'Erov'
"And more importantly, where are we going to find another principal violinist the night before we leave? "Violin Concerto in E minor" Sheeran can't do both."

"Do you want to tell the school about her situation first?" Joan asked.

"I just wrote it together." Fan Ning said, "But I really don't know who to report to. You Bologna School is almost like a sieve. I sent the letter to Roy and let her judge for herself."

"This trip to the imperial capital, let's keep an eye on Julius. This girl takes her chief status and artistic reputation very seriously. At least everyone has the same goal before the performance ends. When the concert's effect is settled, the record will be released. The pre-sale sells the amount of banknotes I want, and we will play by ear. For a first-level knowledgeable person, I can bake ten of them with one 'Fire Sun Guidance'... During the sojourn, her life and bedtime are in my hands. I want to see if she can lead me to some clues in the imperial capital."

He took a deep breath and sat down again: "What we need to do now is to completely separate the extraordinary components in the sample, and when the time comes, the evidence will be packed and directly slapped on their faces... Only pure extraordinary substances can be easily studied. Where is the mysterious characteristic."

After this key point is settled, Ms. Hamilton only needs to complete the evidence closed loop of on-site epidemiology and labor occupational injury assessment, and then rely on her and Monroe's rich legal experience to increase the compensation limit.

Many things are just the tip of the iceberg. If this case is successful, it will be meaningful not only to help the families of the victims, but also to protect the rights and interests of the current or potential future workers of the same type.

Joan has always had a strong interest in the science of elixir. At this moment, she took Fanning's unfolded TLC plate to the back of the altar crystal ball repeatedly to observe—this miraculous phenomenon is enough to keep her going for quite a long time. The novelty of time.

"Caron, I didn't expect it to really separate the extraordinary components, but I think it seems that the amount is too small." After looking at it for a while, she asked her question again, "This is what you just used the capillary glass tube to separate the extraordinary components. It’s on the surface, right? I’m afraid it’s less than one ten-thousandth of an amount. Even if you order a few more times, we’ll probably have to work around the clock for several months to separate all the components in the sample one after another. come out…"

"As I said before, thin-layer chromatography is just a detection method." Fanning laughed, "but the real separation... what we need to use is it..."

"I have special separation skills." Fanning pointed to the row of installed column chromatography clamped on the steel bracket.

Joan was looking at these slender glass columns thoughtfully, and suddenly saw Fanning take out a large flask, mixed all the extracts of the three pigments, and then poured out the two elixirs went in.

"Caroun, what are you doing!?" The two girls exclaimed in amazement.

"Separation is already so difficult, how did you get them together?... You also got four samples at once, how can you separate them??"

"That's right... you have a special mixing technique, right?"

"Extraordinary Solvent is quite expensive." Fan Ning explained earnestly, "One is divided into one, and four are also divided. Why not together? Our time is also very precious."

"Is the method you invented so stable and violent?" Joan's eyes widened. "So you can separate all the components no matter how complicated they are?"

"In theory, by gradually adjusting the solvent ratio of the mobile phase, I can separate out all the extraordinary components from "derivation" to "candle"... If the components are too close on the band, for example, I guess the two phases are the same And different types of extraordinary substances will be similar, which requires a longer chromatographic column, a wider cross-sectional area or a gentler elution pressure..."

"Of course, these extraordinary substances cannot undergo transmutation with each other..." Fan Ning pointed to the pile of mini sample tubes with the glass rod in his hand, "I just tried to spot the mixed solution, it's okay, this system is still relatively Simple, and have common components with each other, just don't waste it..."

Especially the "derivative" phase component, the concentration is extremely thin, only the light and shadow brought by the underground building is a little stronger, if they are not put together, Fan Ning is afraid that the size of the final precipitate will not even reach the size of a fingernail...

"Look, I'll explain while I'm operating. When I need to refine some high-ranking elixir in the future, how about you helping me come to the pillars? Can you make money together?" Fan Ning looked at them playfully, and dug up the potion in his hand. Two scoops of specially treated silica gel powder are poured into the mixed stock solution.

He connected the flask of mixed stock solution mixed with silica gel to his modified "rotary evaporator", then turned on the rotor and started the steam heat source.

"The steamed coelomiae lymph fluid has to be continued, and extraordinary solvents are quite expensive... Remember, as long as it can maintain sufficient adsorption, the silica gel should be as little as possible, and the thinner it is when poured into the column, the better the separation effect will be later. If it is too thick, there may be a tailing phenomenon of 'the back catches up with the front'... I directly use the thickest chromatographic column here, and it is also because it will be thinner when it is laid..."

After the solvent evaporated, all these extraordinary components in the bottle were absorbed in the dry silica gel powder, and under the spiritual sense, there was a faint light of various colors.

Fanning took out the sample paper, folded it into a funnel shape and placed it on the column mouth, and then poured these colorful silica powders into the chromatographic column.

"This is cumulane: coelomus lymph volume ratio is 30:1, and drop a drop of beetle instar inactivated ester solvent, we start with the worst solubility ratio, gradually increase the ratio, must not Excessive force... Judging from our previous thin-layer chromatography detection, if the proportion of coelomia lymph fluid is too high at the beginning, the extraordinary components of several phases will be washed out together..."

Sheeran was dizzy from hearing this, but Joan seemed interested. She put on a small white mask and sat next to Fanning, holding her chin, her exposed eyes gleaming.

Fanning sucked up several sections of mixed solvent with a dropper, and squeezed out from the column mouth around the inner wall, gradually melting the previously poured silica gel powder into a floc like the silica gel at the lower end.

"Remember, squeeze in circles like I do, don't drop directly, that will knock out holes on the surface of the sample and affect the separation purity."

When the liquid above the sample had a certain height as a buffer, Fanning connected the glass solvent ball with openings at the upper and lower ends to the top of the column, fixed it with clips, and began to pour a large amount of solvent into it.

Then he closed the topmost glass stopper and turned on the miniature steam blower connected by a rubber tube.

The solvent starts to flush the silica gel column from top to bottom, and starts to drip from the bottom, and the flask takes over.

During this period, Fanning kept sucking up the liquid at the outlet with a capillary glass tube, and dotted it on the silica gel surface of thin-layer chromatography, and put it into the crystal ball of the altar for inspection.

"You're looking at when the first component comes out of the solvent, right?" Joan added some Radiant Essence Liquid to the altar, and then asked, "If I understand correctly, the mixture has started to move downwards, It just takes time."

There was a hint of approval in Fanning's eyes.

This Ms. Joan Niximi has a very high level of understanding. In her previous life, she must be my favorite junior sister in the research group.

More than a minute later, through the crystal ball, Fan Ning saw the iterative black and white light and shadow on the silicone surface.

The extended version of the test tube rack at hand was already filled with 60 test tubes in all directions. He removed the Erlenmeyer flask and replaced it with test tubes to pick them up one by one.

"Why didn't you continue to use the Erlenmeyer flask?" Joan asked.

"Because I don't know when the 'pool' will flow out." Fanning explained, "If I use a Erlenmeyer flask, by the time I find the light and shadow of the 'pool' in the altar, it has already dripped, and it will pollute again. 'Yan'... Therefore, we generally use test tubes to collect in sections by default from the outflow of the first component, which is not only convenient for operation, but also ensures that cross-contamination only occurs in a part of it at most..."

"Now, please help me test the test tubes No. 1-5 by thin-layer chromatography in order to confirm the components in them." He proposed a task to Joan.

Joan followed the method he taught, using a pencil to draw a line on the thin-layer chromatography plate, mark the number, spot the capillary, dip the lower end in the glass bottle developer and climb up, then clamp it out, and observe it in the altar.

"Reporting to Professor Fan Ning, a row of 'evolution' phase lights and shadows of the same height."

"You combine them with the Erlenmeyer flask liquid, and evaporate the solvent on the rotary evaporator. Remember to recycle the solvent and label it with a ratio." Fanning said.

"Okay!" Joan said excitedly.

A few minutes later, she got a large drop of silver-white viscous liquid that was like mercury in the bottle. According to Fanning's request, she sealed it and put it in a lead box.

In the thin-layer chromatography of the liquid in the sixth test tube, Fan Ning found two light shadows on the "runway" with pink and black and white stripes, so he put a discarded mark on it, indicating that only the solvent will be recovered later, and the solvent will not be recovered. Keep the ingredients.

Test tubes 7-18, the phase is a single pink "pool", after evaporating the solvent, a bunch of strange substances in the shape of flowers are obtained.

At this time, Fan Ning began to switch to a solvent with a volume ratio of cumulane:coelomia lymph fluid of 15:1, and inactivated ester of dicida instars was added dropwise.

Test tube No. 24-34, the phase is a single "cocoon". After evaporating the solvent, a beautiful oval green crystal is obtained.

Change the solvent ratio to 5:1, and add 5 drops.

No. 37-50 test tubes, the phase is a single "candle", after evaporating the solvent, a pool of continuously dancing golden liquid is obtained.

Near eleven o'clock in the evening, all four extraordinary components were separated by Fan Ning.

The two girls looked at him with monster-like eyes.

"Caroun, I can't understand how your brain grows more and more now..." Sheeran stared at his face intently. "Perhaps you can also go to the Department of Chemistry of the Polytechnic Institute as an honorary associate professor."

"No... just with his current achievements, he might be able to become the honorary dean of the Polytechnic Institute." Joan seriously shook his head, "Do you know how difficult it is to separate the extraordinary components? All the pharmacists are This is a headache, I can't estimate its value at all, just the detection method of "thin-layer chromatography" is enough to make a big change in the working methods of the elixir masters, and the "chromatography" seems to be a system, it has no Value cap..."

"Caroun, how did you come up with it? Is it the revelation of mysticism in the dream? The 'key' you studied brought it to you?" Joan, wearing a mask, approached Fan Ning, her dark eyes kept blinking .

"When I was in an art museum before, I accidentally noticed the diffusion of the mixed pigment solution on the paper..." Fanning briefly explained the origin of this chromatography phenomenon and the principle of chromatography he "speculated".

Joan listened earnestly and said "hmmm", then turned her head and said, "Xiran, we have to be cautious, these kinds of Beyonder components may not be anything in themselves, but this separation method and effect must not be leaked out..." Her voice was very low, and she looked around the doors and windows nervously.

"It's the first time I saw you turn around to remind others to be careful." Sheeran's smile was filled with playful relief.

But Joan didn't care about her teasing, and continued to say seriously: "...Callon, I still don't know many details, as long as you teach me later, I can always wash your bottles..."

"That's not enough, let's wash it together..." Fan Ning gently put the used glass bottles and test tubes into the sink one by one, "It's very late, time is tight, and we still need to clarify these extraordinary things next. The mysterious properties of matter."

"In this way... Combining their origins, we may be able to deduce many clues related to them..."

　Thanks to Klebsiella's monthly ticket reward on August 8~Thanks to a cat for eating dried fish, Klebsiella, monitor 21, and frank's monthly ticket~
　　
　
(End of this chapter)