Old-time musicians

"Well…"

Sheeran and Qiong followed the direction of Fan Ning's finger and looked towards the end of the table.

The sink, washbasin, brush, alcohol, washing powder, steam oven...all the utensils are neatly placed there.

"Okay, it's not impossible." Qiong curled her lips, "But I'm mainly curious about what you want to do. Maybe you don't believe it, but distillation, crystallization, centrifugation and other methods are really inseparable from these extraordinary components. In very few cases, the knowledgeable people of high rank and above rely on their own powerful inspiration control and use certain means to pull out the chaotic transmutation products, and this is still incomplete separation and purification."

Fanning has already begun his operation.

"Stay away first. You are not wearing a mask. It will be difficult to expel this stuff after it is inhaled into your lungs."

He cut open the silica powder packaging bag, poured half of the volume into a large glass with a capacity of more than one liter, and then poured the finely ground fumigation particles and calcined particles into it according to the volume ratio of 5% and 2% respectively. The black bones of the ash-spotted glands.

Sultivated particles are a kind of migrating substance. In certain dreamland areas where the light and shadow are close to noon, those who know can collect a heavy and shining crystal on the surface of the dust. When you slide your fingers through it, you can feel the affinity of inspiration for it.

The black light spot is a kind of "trace spirit" in the migration. This kind of material is considered to be "the leftovers of the glow and the memories of history that are constantly drifting downward." Although there are living things in the migration, characteristics, but they are far inferior to 'moving creatures' and cannot affect the waking world.

The "skin of the end", the production carrier of common curse seals, also comes from a plant-like trace spirit in the migration.

Dust is floating in the air, and after mixing, the silicone has the ability to absorb extraordinary substances similar to ordinary substances.

Fan Ning picked up a large Erlenmeyer flask with both hands and raised it to his eyes for observation.

"What is this?" Sheeran was very curious.

"Water, and 8% industrial cellulose that has been roughly carboxymethylated." Fan Ning said, "You can understand it as a thickening or binder that is not easy to use and is quite expensive."

"Are you a transfer from St. Lenia Polytechnic?" The two girls touched their heads in confusion.

"It should be ready after letting it sit for two days." Fan Ning pulled out the stopper, poured the supernatant inside into another glass, then added the processed silicone powder, and stirred it into a large ball of light gray with a little transparency. A viscous slurry with a shiny texture.

Then he took out a 10x10 cm glass plate, scooped out a large spoonful of slurry and placed it in the middle, tilting it left and right to allow the slurry to flow evenly on the surface under the action of gravity.

"What does this have to do with isolating extraordinary components? Caroln, are you making pastry? Is this edible?"

Seeing Fan Ning put the square glass plates coated with glitter slurry into the oven one after another, Qiong couldn't help but ask.

"Aren't you full yet?" Fan Ning closed the oven door, turned on the steam heater, then returned to the table, reached out and pulled a small handful of cotton from nearby.

On the steel bracket in front of the wall, a long glass column has been fixed with clips. Its length is nearly one meter, but its diameter is only three centimeters. It has a frosted opening at the top and a glass bolt at the bottom.

Fan Ning threw the cotton in, gently pressed it against the bottom outlet with a long glass rod, and then spread a layer of washed river sand on top.

He unscrewed another brown bottle, poured the gray clear liquid into another part of the silica powder, and stirred it into a slurry.

There was a smell slightly like something rotten in the air.

"What is this?" Sheeran asked curiously.

"Blight Moth," Qiong explained, "a special solvent with a low boiling point that he screened before. It is made from the migrating trace spirit 'Blight Moth' and some light petroleum components. It has relatively poor solubility. It is narrower and has weaker background interference with spiritual perception. It has some applications in extraordinary material crystallization."

"But I still don't understand." She couldn't help but ask again, "Caron, why don't you try to dissolve the sample with methane and mix it with silica gel? These two things can't be used. Fusion is just a ball of sticky slurry. Also, what is the purpose of making this pillar of yours thin and long?"

"I'm loading 'column chromatography.'" Fan Ning raised the large glass of slurry, poured it into the column with a glass rod, and then used a dropper to absorb a few pieces of methane, rotating it at the top of the column and squeezing it out, removing the residue remaining on the wall. Wash down the silicone powder and plug the top.

A mini steam blower connected to the rubber tube began to hum, compacting the silicone slurry in the column downwards.

Phane flowed out from below, and he caught it with a cup and poured it into the top again. After repeated operations several times, a large silicone column with a contradictory sense of darkness and sparkle appeared in the glass. There is a seal of a few centimeters deep of fentanyl liquid.

"Column chromatography? Did you invent this? This column...can separate extraordinary components?" Joan looked closely at it in disbelief.

"You'll find out later." Fan Ning smiled mysteriously.

Fan Ning filled several such pillars, some thick and thin, in turn. After plugging them, they were clamped in the air with clamps and lined up on the steel supports.

A few people wandered around casually for a while. In the evening, Fan Ning opened the oven door and took out the tray. The silicone slurry on the square glass had become dry and smooth.

He took out a glass cutter, drew straight lines two centimeters apart on the back, and then "bang bang" broke it into glass strips.

Before Joan asked, he took the initiative to explain: "This is thin layer chromatography... I will first take an extremely small sample and use it to detect the distribution of extraordinary components in it, and then formulate a separation strategy and use column chromatography for separation."

Whether it is column chromatography or thin layer chromatography, their separation principles are the same:

Under the flushing of the mobile phase, different substances move through the stationary phase at different speeds - the speed here is strictly speaking the speed of the "adsorption-desorption-re-adsorption-re-desorption" cycle process.

In Fanning's previous life, the common stationary phase was silica gel, and the mobile phase was organic solvents such as petroleum ether/ethyl acetate/dichloromethane. In this world where mysticism is popular, the stationary phase he chose is silica gel treated with extraordinary substances, and the mobile phase is the mothane he selected, as well as the coelenterate lymph fluid and the inactivation ester of the worm.

After preparing the column chromatography and thin layer chromatography, he finally started to process the samples.

Fanning was still a little excited and nervous at the moment. Separating the extraordinary components was only a small part of the meaning. More importantly, if the chromatography principle could be reproduced in this world, those so-called "waste formulas" would become high-priced high-level spiritual agents after separating the lethal byproducts of chaotic transmutation!

He put the three pigments into the separatory funnel one by one, first added water to stir into a turbid suspension, then poured in the same volume of coelenterate lymph fluid, shook, let it stand, and separated them into layers.

This is an extraordinary solvent with a strong and wide solubility. At this time, most of the mixed extraordinary components in the pigment entered this layer. Under spiritual perception, the dilute solution showed a chaotic light and shadow with mottled colors.

Open the tap, separate the liquid, add it again, shake it and let it stand to separate…

Joan has been watching for so long, and finally there is something she can understand, so she turned to Sheeran and said: "… His extraction operation is more standard than mine, and he is the most skilled musician I have ever seen… I now think he must have secretly gone to the Chemistry Department of the Polytechnic Institute to study…"

The sample became a stock solution, and Fanning took out several micro sample tubes the size of the tip of his little finger, labeled them, and sealed them one by one for preservation.

Then, he picked up the measuring cylinder.

"Configuration of mobile phase… Try the ratio of mothane and coelenterate lymph fluid to 5:1, and then add five drops of inactivating ester of worms."

After Fanning finished measuring, he poured the mixed solvent into a cylindrical glass bottle. He used very little, and the depth of the liquid in the bottle was only half a centimeter.

This bottle is where the thin layer chromatography will be developed later.

He took out a piece of thin layer chromatography that had been divided into a rectangle, drew a straight line with a pencil one centimeter from the bottom of the silica gel surface, dotted three points on the straight line, and wrote small words 1, 2, and 3.

Use a capillary glass tube to gently absorb the original solution of the watch factory pigment, the original solution of the poorhouse pigment (yellow branch), and the original solution of the underground building pigment, and click on the positions 1, 2, and 3 on the straight line in turn.

Finally, he picked up the thin layer chromatography glass with tweezers, put it into the cylindrical bottle, let the pencil line be above the liquid surface, and closed the bottle cap.

Just like water soaking the paper surface, the mixed extraordinary solvent began to climb on the silica gel surface. It was visible to the naked eye that a darker area was spreading from bottom to top.

A minute later, when the dark side was about to climb to the top of the glass plate, Fanning clipped it out.

The low-boiling point extraordinary solvent quickly evaporated in the air, and the dark side disappeared. The silica gel on this glass plate looked no different from before. Even with Fanning's spiritual perception, he could hardly see the heterogeneous light and shadow on it.

Because the concentration was too thin.

"What happened?" Joan and Hiran were confused, but when Fanning moved the glass plate to the gem altar that had already started to operate, they saw a surprising scene through the crystal ball: the top of the three "runways" was a split and overlapping striped light and shadow, and golden spots appeared in the middle of No. 2 and No. 3!

As expected by Fanning, although his spiritual perception could not distinguish due to the low concentration of extraordinary components, he could use the form of crystal ball + altar to strengthen his spiritual perception.

Just like the method used to observe thin-layer chromatography in the previous life, there are ultraviolet lights or iodine vapor...

In this mystical world, the way to observe extraordinary substances is spiritual perception + secret instrument. Of course, if further characterization is to be carried out, it may still be necessary to rely on the hidden knowledge accumulation of the knower after separation to judge-the moving substance may be difficult to understand as a molecule or pure substance.

For the purpose of "revitalizing the waste spiritual potion formula", it is enough to be able to observe and separate.

Joan is also a knower with a talent in spiritual potion, and she immediately interpreted this phenomenon.

Sample No. 1: The pigment of the watch factory, which only contains a single extraordinary component of a certain "derivative" phase.

Samples No. 2 and 3: Pigments of the workhouse and underground buildings, in addition to the "derivative" phase component, there is also a certain "candle" phase component separated by Fanning!

"Carlon, what is your invention called... thin layer chromatography? I didn't expect this method to be really useful!" Looking at Fanning who was thinking, Joan's tone was very excited.

"The successful separation of mixed extraordinary substances is extremely rare. I didn't expect you to succeed in your first practice of spiritual medicine, and it was very low-cost and short-time. You are so lucky!"

"Luck?" Fanning smiled lightly, "You will know it soon. My method can be reproduced in most cases."

"Change the ratio of mothane and coelenterate lymph fluid to 15:1, and add two drops of worm inactivation ester." He measured the new mobile phase ratio and poured it into the cylindrical glass bottle.

He drew a straight line on the second thin-layer chromatography, wrote down the number, and used a capillary glass tube to absorb the original liquid and clicked it again.

At this time, Fanning wrote an extra number 4: the spiritual potion taken by the principals.

Immerse the lower end of the straight line in the cylindrical bottle in the same way, and clamp it out when the extraordinary solvent is about to climb to the top.

The next second, Joan stared at the crystal ball in the altar with a look of shock on her face.

Sample No. 1: The pigment of the watch factory, the front end is still "Yan", but it has not run that far.

Samples No. 2 and No. 3: The pigment of the poorhouse and underground buildings, "Yan" also did not run that far, and the "Zhu" phase component basically did not "run" and remained at the starting position.

Even more surprising is sample No. 4: the spiritual potion taken by the principal, which has three kinds of light and shadow, the top is "Yan", and the following are the pink "Pool" and the green "Cocoon", but they are all above the previous "Zhu".

There are actually four extraordinary substances that appear independently on this so-called "thin-layer chromatography"!

Qiong shouted out in excitement. Fortunately, the third floor was empty and the building had good sound insulation.

Fanning was also vaguely excited at this moment, because he found that his conjecture was correct. The principle of chromatography was indeed applicable in this mystical world.

The only difference is that the flow rate of ordinary substances in the chromatogram depends on their polarity, or the degree of molecular symmetry. In a positive column environment, the more symmetrical the smaller the polarity, the faster it flows, and vice versa, the more asymmetric the greater the polarity, the slower it flows. By adjusting the mixing ratio between several strong polar and weak polar solvents, their unfolding can be controlled to put them in the most appropriate position.

As for extraordinary substances... Fanning initially discovered that their flow rate seems to be related to the overall order of the seven phases! Under the current extraordinary solvent configuration, the fastest unfolding is "derivation" and the slowest is "candle"... His idea is also based on the solubility of extraordinary solvents, combining high and low performance solvents, and controlling the unfolding by controlling the ratio...

After summarizing the preliminary mystical laws in his mind, Fanning's brows gradually frowned.

First of all, there is the problem of "candle"... Since the yellow pigment he cut off with the crowbar in the underground building happened to be a piece of yellow pigment, the Langevni brand pigment he chose this time was also yellow. Now it seems that they share the ingredient of "candle".

Moreover...

His eyes were fixed on the "derivative" light and shadows on the thin layer chromatography silica gel.

Both pigments and spiritual potions have "derivatives"? Is this a coincidence or...?

Joan obviously also realized this problem: "... Carloen, the different stock solution "runways" here, the "derivative" light and shadows all climb to the same height... Can this mean that they are the same extraordinary substance?"

"It is very likely, but a more rigorous verification method is needed."

In order to make the "derivative" climb lower, Fanning changed the mobile phase combination with lower solubility performance - mothane: coelenterate lymph fluid = 30:1, and dripped a drop of instar worm passivation ester.

He took out the third thin layer chromatography glass plate, and this time he added a mixing point of several samples on the basis of the previous one.

As they climbed up again, they found that at this time, the "candle" and "cocoon" were almost not running, and they were all mixed at the starting point. The "pool" went up a little, and the "Yan" was in the lower middle position. Moreover, the height of the "Yan" in all the tracks was still exactly the same!

"The spiritual potion that the principals took... contains extremely small amounts of the radioactive "Yan" phase extraordinary components in the pigment of the Jinlangnia Watch Factory!?!?" Fanning was dumbfounded.

The spiritual potion maker, Dean Graham, a professor of the Department of Chemistry of the Institute of Technology? ...

The pigment supplier of the watch factory, Reradium Chemical Trading Company? ...

The situation in the imperial capital...

The infiltration of the School of Reconciliation...

The Special Patrol Office with an elusive stance...

The investigator who went crazy because of the "Spring in the Painting"...

A lot of fragments of information immediately emerged in Fanning's mind.

Sheeran's face showed deep worry: "...So, Principal Stenike and Vice Principal Huxley, how long have you been taking this elixir?"

Fanning tried to calculate the time that had passed since the premiere of the "First Symphony".

Today is the night before the departure for the performance in the imperial capital!

"Maybe...it's been almost two months?"